Lipase-catalysed esterification in supercritical carbon dioxide and in hexane

Bioorganic and Medicinal Chemistry Letters

Volumn 7, Issue 3, 1997, Pages 259-262

  Vija, Heiki ^a   Telling, Artur ^a   Tõugu, Vello ^a  

^a NATIONAL INSTITUTE OF CHEMICAL PHYSICS AND BIOPHYSICS   (Estonia)

Author keywords

[No Author keywords available]

Indexed keywords

ISOPENTYL ACETATE; TRIACYLGLYCEROL LIPASE;

ARTICLE; DRUG SYNTHESIS; ENZYME ACTIVITY; ESTERIFICATION; IN VITRO STUDY; METHODOLOGY; REACTION ANALYSIS;

EID: 0031552090     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0960-894X(96)00614-2     Document Type: Article

References (18)

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13. 8. Reaction was carried out in a stainless steel reactor with internal volume 100 ml. Before each experiment the reactor was opened and a precise amount of the substrates, water and the enzyme was introduced within the reactor. After sealing, the system was pressurized by pumping liquid carbon dioxide (99.5 % pure Eesti AGA, Estonia).by a syringe pump (DuPont Instruments, USA) to the desired pressure and the reactor was isolated from the circuit by closing valve 1. Samples were withdrawn from the reaction mixture and depressurized through a frit restrictor (AS Englo, Estonia). Samples were collected into methanol. Two HPLC valves in the system enabled to wash the restrictor between sample collecting. Esterification in n-hexane was carried out in a screw capped flask containing 10 ml of the solvent. The mixture was stirred on a magnetic stirrer at room temperature. Samples of the reaction media were periodically analysed by a gas chromatograph Chrom 5 (Laboratorni Pristroje, Praha, Czechia) equipped with a capillary column Nordion NB-20M (0.5μ, 25 m × 0.32 mm) and a FID detector at 150°C. Helium (0.5 ml/min) was used as a carrier. The temperature gradient was from 55°C to 120°C at 10°C/min. The molar ratio of isoamyl acetate and isoamyl alcohol was determined. The lipolytic enzymes, Novozym and Lipolase were kindly provided by Novo Nordisk, Denmark.
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