Highly photoresistant chemosensors using acridone as fluorescent label

Tetrahedron Letters

Volumn 38, Issue 9, 1997, Pages 1489-1492

  Bahr, Nicolaus ^a   Tierney, Emily ^a   Reymond, Jean Louis ^a  

^a SCRIPPS RESEARCH INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACRIDONE DERIVATIVE; FLUORESCENT DYE; MONOCLONAL ANTIBODY;

ALKYLATION; ARTICLE; BLEACHING; FLUORESCENCE; IRRADIATION; SENSOR;

EID: 0031550821     PISSN: 00404039     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0040-4039(97)00137-8     Document Type: Article

References (19)

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18. 50 is defined here as the concentration inducing 50% of the maximum fluorescence increase.
19. exc = 356 nm, set to give maximum fluorescence reading (2.0) at 445 nm for 2 μM free 2. Aliquots of 4 μL of properly prediluted solutions of analyte 1, 4 or 5 were added to 2 mL of sensor (1 μM 2 + 0.5 μM antibody 34F7 and/or 0.5 μM antibody 83B8) in aqueous 160 mM NaCl, 10 mM phosphate, pH 7.4. The solution was then mixed using a 1 mL pipetter and allowed to stand for 2 minutes before recording fluorescence.