Conjugates of Stimuli-Responsive Polymers and Biomolecules: Random and Site-Specific Conjugates of Temperature-Sensitive Polymers and Proteins

Macromolecular Symposia

Volumn 118, Issue , 1997, Pages 553-562

  Hoffman, Allan S ^a   Stayton, Patrick S ^a   Shimoboji, Tsuyoshi ^a   Chen, Guohua ^a   Ding, Zhongli ^a   Chilkoti, Ashutosh ^a   Long, Cindi ^a   Miura, Morikazu ^a   Chen, Jingping ^a   Park, Taegwan ^a   Monji, Nobuo ^b   Cole, Carol Ann ^b   Harris, J Milton ^c   Nakamae, K ^d  

^a University of Washington   (United States)

^b Genetic Systems Sanofi Diagnostics   (United States)

^c UNIVERSITY OF ALABAMA IN HUNTSVILLE   (United States)

^d KOBE UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 0031528744     PISSN: 10221360     EISSN: None     Source Type: Journal    
DOI: 10.1002/masy.19971180173     Document Type: Article

References (22)

1. Tanaka, T., "Gels", Sci. Amer., 244, 124-138 (1981)
2. Osada, Y. and S.B. Ross-Murphy, "Intelligent Gels", Sci. Amer., 268, 82-87 (1993)
3. Hoffman, A.S., "Intelligent Polymers in Medicine and Biotechnology", Artif. Organs, 19, 458-467 (1995); also Macromol. Symp., 98, 645-664 (1995)
4. Hoffman, A.S., "Intelligent Polymers in Medicine and Biotechnology", Artif. Organs, 19, 458-467 (1995); also Macromol. Symp., 98, 645-664 (1995)
5. Feijen, J., I. Feil, F.J. van der Gaag, Y.H. Bae and S.W. Kim, "Thermosensitive Polymers and Hydrogels Based on N-isopropylacrylamide", 11th European Conf. on Biomtls., 256-260 (1994)
6. Monji, N. and A.S. Hoffman, "A Novel Immunoassay System and Bioseparation Process Based on Thermal Phase Separating Polymers", Appl. Biochem. and Biotech., 14, 107-120 (1987)
7. Fujimura, M., T. Mori and T. Tosa, "Preparation and Properties of Soluble-Insoluble Immobilized Proteases", Biotech. Bioeng., 29, 747-752 (1987)
8. Nguyen, A.L. and J.H.T. Luong, "Synthesis and Applications of Water-Soluble Reactive Polymers for Purification and Immobilizatipn of Biomolecules", Biotech. Bioeng., 34, 1186-1190 (1989)
9. Taniguchi, M., M. Kobayahi and M. Fujii, "Properties of a Reversible Soluble-Insoluble Cellulase and Its Applicatipn to Repeated Hydrolysis of Crystalline Cellulose", Biotech. Bioeng., 34, 1092-1097 (1989)
10. Monji, N., C-A. Cole, M. Tam, L. Goldstein, R.C. Nowinski and A.S. Hoffman, "Application of a Thermally-Reversible Polymer-Antibody Conjugate in a Novel Membrane-Based Immunoassay", Biochem. and Biophys. Res. Comm., 172, 652-660 (1990)
11. Monji, N. C.A. Cole, and A. S. Hoffman, "Activated, N-Substituted Acrylamide Polymers for Antibody Coupling: Application to a Novel Membrane-Based Immunoassay", J. Biomtls. Sci. Polymer Ed., 5, 407-420 (1994)
12. Chen, J.P. and A.S. Hoffman, "Polymer-Protein Conjugates: Affinity Precipitation of Human IgG by Poly(N-Isopropyl Acrylamide)-Protein A Conjugates", Biomtls., 11, 631-634 (1990)
13. Park, T. G. and A. S. Hoffman, "Synthesis and Characterization of a Soluble, Temperature-Sensitive Polymer-Conjugated Enzyme", J. Biomtls. Sci. Polymer Ed., 4,. 493-504 (1993)
14. Chen, G.H., and A.S. Hoffman, "Preparation and Properties of Thermo-Reversible, Phase-Separating Enzyme-Oligo(NIPAAm) Conjugates", Bioconj. Chem. , 4, 509-514 (1993)
15. Ding, Z.L., G.H. Chen, and A.S. Hoffman, "Synthesis and Purification of Thermally-Sensitive Oligomer-Enzyme Conjugates of Poly(NIPAAm)-Trypsin", Bioconj. Chem., 7, 121-125 (1995)
16. Chen, G.H. and A.S. Hoffman, "A New Temperature-and pH-Responsive Copolymer for Possible Use in Protein Conjugation", Macromol. Chem. Phys., 196, 1251-1259 (1995)
17. Takei, Y.G., T. Aoki, K. Sanui, N. Ogata, T. Okano, and Y. Sakurai, "Temperature-responsive Bioconjugates. 1. Synthesis of Temperature-Responsive Oligomers with Reactive End Groups and their Coupling to Biomolecules", Bioconj. Chem., 4, 42-46 (1993)
18. Takei, Y.G., T. Aoki, K. Sanui, N. Ogata, T. Okano and Y. Sakurai, "Temperature-responsive Bioconjugates. 2. Molecular Design fro Temperature-modulated Bioseparations", Bioconj. Chem., 4, 341-346 (1993)
19. Takei, Y.G., M. Matsukata, T. Aoki, K. Sanui, N. Ogata, A. Kikuchi, Y. Sakurai and T. Okano, "Temperature-responsive Bioconjugates. 3. Antibody-Poly(N-isopropylacrylamide) Conjugates for Temperature-Modulated Precipitations and Affinity Bioseparations", Bioconj. Chem., 5, 577-582 (1994)
20. Matsukata, M., Y. Takei, T. Aoki, K. Sanui, N. Ogata, Y. Sakurai and T. Okano, "Temperature Modulated Solubility-Activity Alterations for Poly(N-Isopropylacrylamide)-Lipase Conjugates", J. Biochem., 116, 682-686 (1994)
21. Chilkoti, A., G.H. Chen, P.S. Stayton and A.S. Hoffman, "Site-Specific Conjugation of a Temperature-Sensitive Polymer to a Genetically-Engineered Protein", Bioconj. Chem., 5, 504-507 (1994)
22. Stayton, P.S., T. Shimoboji, C. Long, A. Chilkoti, G. Chen, J.M. Harris and A.S. Hoffman, "Control of Protein-Ligand Recognition Using a Stimuli-Responsive Polymer", Nature , 378, 472-474 (1995)