Co-isolation of high-quality DNA and RNA from coenocytic green algae

Plant Molecular Biology Reporter

Volumn 15, Issue 3, 1997, Pages 263-272

  La Claire II, John W ^a   Herrin, David L ^a  

^a UNIVERSITY OF TEXAS AT AUSTIN   (United States)

Author keywords

cDNA cloning; Green algae; Nucleic acid purification; Plants

Indexed keywords

ALGAE; CHLOROPHYTA;

EID: 0031478699     PISSN: 07359640     EISSN: None     Source Type: Journal    
DOI: 10.1023/a:1007486419576     Document Type: Article

References (11)

1. Coleman, A.W., L.J. Goff, J.R. Stein-Taylor. 1989. Algae as Experimental Systems. A.R. Liss, Inc., New York.
2. Coyer, J.A., D.L. Steller, R.S. Alberte. 1995. A field-compatible method for extraction of fingerprint-quality DNA from Macrocystis pyrifera (Phaeophyceae). J. Phycol. 31:177-180.
3. Grierson, D. 1990. Gel electrophoresis of RNA, pp. 1-49 in Gel Electrophoresis of Nucleic Acids. A Practical Approach. D. Rickwood and B.D. Hames, eds., IRL Press, Oxford.
4. Harris, E.H. 1989. The Chlamydomonas Sourcebook. Academic Press, San Diego.
5. John, M.E. 1992. An efficient method for isolation of RNA and DNA from plants containing polyphenolics. Nucleic Acids Res. 20:2381.
6. La Claire, J.W., II, G.C. Zuccarello, S. Tong. 1997. Abundant plasmid-like DNA in various members of the Siphonocladales and Cladophorales (Chlorophyta). J. Phycol. 33 (in press).
7. Manning, K. 1991. Isolation of nucleic acids from plants by differential solvent precipitation. Analyt. Biochem. 195:45-50.
8. Murray, M.G., W.F. Thompson. 1980. Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res. 8:4321-4325.
9. Roell, M.K., D.E. Morse. 1991. Fractionation of nuclear, chloroplast, and mitochondrial DNA from Polysiphonia boldii (Rhodophyta) using a rapid and simple method for the simultaneous isolation of RNA and DNA. J. Phycol. 27:299-305.
10. Sambrook, J., E.F Fritsch, T. Maniatis. 1989. Molecular Cloning. A Laboratory Manual. Second Edition. Cold Spring Harbor Press, N.Y.
11. Staves, M.P., J.W. La Claire II. 1985. Nuclear synchrony in Valonia macrophysa (Chlorophyta): light microscopy and flow cytometry. J. Phycol. 21:68-81.