Purification and properties of buffalo (Bubalus bubalis) erythrocyte hexokinase

Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology

Volumn 118, Issue 2, 1997, Pages 395-401

  De Cerqueira Cesar, Marcelo ^a   Colepicolo, Pio ^a   Rosa, Claudete Deguirmendjian ^b   Rosa, Rubens ^a  

^a UNIVERSITY OF SÃO PAULO   (Brazil)

^b SÃO PAULO STATE UNIVERSITY UNESP   (Brazil)

Author keywords

Buffalo; Erythrocyte; Glucose 6 P; Hexokinase; Inhibition; Kinetics; Mechanism; Phosphorylation

Indexed keywords

HEXOKINASE;

ANIMAL CELL; ARTICLE; BUFFALO; ENZYME ANALYSIS; ENZYME MECHANISM; ENZYME PURIFICATION; ERYTHROCYTE; IMMUNOBLOTTING; NONHUMAN; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; RABBIT;

ANIMALIA; BUBALUS; BUBALUS BUBALIS; MAMMALIA; ORYCTOLAGUS CUNICULUS;

EID: 0031467027     PISSN: 03050491     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0305-0491(97)00161-2     Document Type: Article

References (40)

1. Baijal, M.; Wilson, J.E. Studies on the binding of the nucleotides by rat brain hexokinase. Arch. Biochem. Biophys. 218: 513-524;1982.
2. Bradford, M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254;1976.
3. Casazza, J.P.; Fromm, H.J. Studies on the mode of sugar-phosphate product inhibition of brain hexokinase. Arch. Biochem. Biophys. 177:480-487;1976.
4. Colowick, S.P. The Enzymes. New York: Academic Press; 1973.
5. De Cerqueira Cesar, M.; Fernandes, L.C.; Curi, R.; Sassaki, K. T.; Rosa, C.D.; Rosa, R. Glucose metabolism in the erythrocytes of the buffalo (Bubalus bubalis). Comp. Biochem. Physiol. (In press)
6. Easterby, J.S.; O'Brien, M.J. Purification and properties of pig-heart hexokinase. Eur. J. Biochem. 38:201-211;1973.
7. Fomaini, G.; Dachà, M.; Magnani, M.; Stocchi, V. Molecular forms of red blood cell hexokinase. Mol. Cell. Biochem. 49: 129-142;1982.
8. Fromm, H.J.; Silverstein, E.; Boyer, P.D. Equilibrium and net reaction rates in relation to the mechanism of yeast hexokinase. J. Biol. Chem. 239:3645-3652;1964.
9. Gonzalez, C.; Ureta, T.; Babul, J.; Rabajille, E.; Niemeyer, H. Characterization of isozymes of adenosine triphosphate: D-hexose 6-phosphotransferase from rat liver. Biochemistry 6: 460-468;1967.
10. Grossbard, L.; Schimke, R. Multiple hexokinases of rat tissues. Purification and comparison of soluble forms. J. Biol. Chem. 241:3546-3560;1966.
11. 18O isotope effects for hexokinase-catalyzed phosphoryl transfer from ATP. Biochemistry 30:3634-3639;1991.
12. Kalomenopoulou, M.; Beis, I. Studies on the pigeon red blood cell metabolism. Comp. Biochem. Physiol. 956:677-684; 1990.
13. Katzen, H.M.; Schimke, R.T. Multiple forms of hexokinase in the rat: tissue distribution, age, dependency and properties. Proc. Natl. Acad. Sci. USA 54:531-536;1966.
14. Laemmli, U.K. Cleavage of Structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685; 1970.
15. Magnani, M.; Dachà, M.; Stocchi, V.; Ninfali, P.; Fornaini, G. Rabbit red blood cell hexokinase. Purification and properties. J. Biol. Chem. 255:1752-1756;1980.
16. Magnani, M.; Stocchi, V.; Serafini, N.; Piatti, E.; Dachà, M.; Fomaini, G. Multiple forms of hexokinase in mammalian red blood cells. Biochem. Int. 4:673-677;1982.
17. Magnani, M.; Stocchi, V.; Dachà, M.; Fomaini, G. Regulatory properties of rabbit red blood cell hexokinase at conditions close to physiological. Biochim. Biophys. Acta 804:145-53; 1984.
18. Misset, O.; Bos, O.F.M.; Opperdoes, F.R. Glycolytic enzymes of Trypanosoma brucei. Simultaneous purification, intraglycosomal concentrations and physical properties. Eur. J. Biochem. 157:441-453;1986.
19. Ninfali, P.; Magnani, M.; Dachà, M.; Stocchi, V.; Fornaini, G. Rabbit erythrocyte hexokinase: studies on the kinetic mechanisms. Biochem. Int. 1:574-583;1980.
20. Ning, J.; Purlich, D.L.; Fromm, H.J. Studies on the kinetic mechanism and allosteric nature of bovine brain hexokinase. J. Biol. Chem. 244:3840-3846;1969.
21. Oakley, B.R.; Kirsch, D.R.; Morris, N.R. A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gel. Anal. Biochem. 105:361-363;1980.
22. O'Sullivan, W.J.; Smithers, G.W. Stability constants for biologically important metal-ligand complexes. Methods Enzymol. 63:294-336;1979.
23. Perrella, F.W. EZ-Fit: A practical curve-fitting microcomputer program for the analysis of enzyme kinetic data on IBM-PC compatible computers. Anal. Biochem. 174:437-447;1988.
24. Purich, D.L.; Fromm, H.J. The kinetics and regulation of rat brain hexokinase. J. Biol. Chem. 246:3456-3463;1971.
25. Rees, B.B.; Ropson, I.J.; Hand, S.C. Kinetic properties of hexokinase under near-physiological conditions. Relation to metabolic arrest in Artemia embryos during anoxia. J. Biol. Chem. 264:15410-15417;1989.
26. Serafini, G.; Magnani, M.; Stocchi, V.; Dachà, M.; Fomaini, G. Rat red blood cell hexokinase purification, properties and age dependence, Mol. Cell. Biochem. 69:179-85;1986.
27. Sols, A.; Crane, R.K. Substrate specificity of brain hexokinase. J. Biol. Chem. 210:581-595;1954.
28. Stocchi, V.; Magnani, M.; Novelli, G.; Dachà, M.; Fomaini G. Pig red blood cell hexokinase: Evidence for the presence of hexokinase types II and III, and their purification and characterization. Arch. Biochem. Biophys. 226:365-376;1983.
29. -2 and other ions in solution. Calculation of the true concentrations of species present in mixtures of associating ions. Biochem. J. 159:1-5;1976.
30. Tielens, A.G.M.; van den Heuvel, J.M.; van Mazijk, H.J.; Wilson, J.E.; Shoemaker, C.B. The 50-kDa glucose 6-phosphate-sensitive hexokinase of Schistosoma mansoni. J. Biol. Chem. 269:24736-24741;1994.
31. Towbin, H.T.; Staehelin, T.; Gordon, J. Electrophoretic transfer of proteins from polyacrilamide gels to nitrocellulose sheets: Procedure and some applications. Proc. Natl. Acad. Sci. USA 76:4350-4354;1979.
32. Tsai, H.J.; Wilson, J.E. Functional organization of mammalian hexokinases: Characterization of chimeric hexokinases constructed from the N- and C-terminal domains of the rat type I and type II isozymes. Arch. Biochem. Biophys. 316:206-214; 1995.
33. White, T.K.; Wilson, J.E. Rat brain hexokinase: Location of the allosteric regulatory site in a structural domain at the N-terminus of the enzyme. Arch. Biochem. Biophys. 259:402-411;1987.
34. White, T.K.; Wilson, J.E. Isolation and characterization of the discrete N- and C-terminal halves of rat brain hexokinase: Retention of full catalytic activity in the isolated C-terminal half. Arch. Biochem. Biophys. 274:375-393;1989.
35. White, T.K.; Wilson, J.E. Binding of nucleoside triphosphates, inorganic phosphate and other polyanionic ligands to the N-terminal region of rat brain hexokinase: Relationship to regulation of hexokinase activity by antagonistic interactions between glucose 6-phosphate and inorganic phosphate. Arch. Biochem. Biophys. 277:26-34;1990.
36. Wilson, J.E. Ligand-induced conformational changes of rat brain hexokinase: Studies with hexoses, hexose 6-phosphates and nucleoside triphosphates leading to development of a new model for the enzyme. Arch. Biochem. Biophys. 185:88-99; 1978.
37. Wilson, J.E. Ligand-induced conformational changes of rat brain hexokinase: their role in determining the substrate specificity for hexoses and inhibitory effectiveness of hexose 6-phosphates. Arch. Biochem. Biophys. 196:79-87;1979.
38. Wilson, J.E. Regulation of mammalian hexokinase activity. In: Beitner, R., (ed). Regulation of carbohydrate metabolism. Boca Raton, FL: CRC; 1984:45-85.
39. Wilson, J.E. Hexokinases. Rev. Physiol. Biochem. Pharmacol. 126:65-198;1995.
40. Wilson, J.E.; Schwab, D.A. Functional interaction of hexokinase with ATP requires participation by both small and large lobes of the enzyme: Implications for other proteins using the actin fold as a nucleotide binding motif. FASEB J. 10:799-801;1996.