Purification of Pf155/RESA antigen from supernatants of in vitro Plasmodium falciparum cultures by continuous elution electrophoresis

Journal of Immunological Methods

Volumn 208, Issue 1, 1997, Pages 29-34

  Dubois, B ^a,b   Deloron, P ^a,b  

^a INSERM   (France)

^b Inst de Med et d'Epidemiol A   (France)

Author keywords

Continuous elution electrophoresis; Gel filtration; Pf155 RESA; Plasmodium falciparum; Purification

Indexed keywords

PARASITE ANTIGEN;

ANTIGEN DETECTION; ARTICLE; ELECTROPHORESIS; GEL FILTRATION; IMMUNOBLOTTING; NONHUMAN; PLASMODIUM FALCIPARUM; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN PURIFICATION; SUPERNATANT;

ANIMALS; ANTIGENS, PROTOZOAN; ANTIGENS, SURFACE; CHROMATOGRAPHY, GEL; CULTURE MEDIA; ELECTROPHORESIS, POLYACRYLAMIDE GEL; IMMUNOBLOTTING; PLASMODIUM FALCIPARUM; PROTOZOAN PROTEINS;

PLASMODIUM FALCIPARUM;

EID: 0031461194     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(97)00122-1     Document Type: Article

References (18)

1. Aikawa M., Torii M., Sjölander A., Berzins K., Perlmann P., Miller L.H. Pf155/RESA antigen is localized in dense granules of Plasmodium falciparum merozoites. Exp. Parasitol. 71:1990;326.
2. Berzins K., Wahlgren M., Perlmann P. Studies on the specificity of anti-erythrocyte antibodies in the serum of patients with malaria. Clin. Exp. Immunol. 54:1983;313.
3. Brown G.V., Culvenor J.G., Crewther P.E., Bianco A.E., Coppel R.L., Saint R.B., Stahl H.D., Kemp D.J., Anders R.F. Localization of the ring-infected erythrocyte surface antigen (RESA) of Plasmodium falciparum in merozoites and ring-infected erythrocytes. J. Exp. Med. 162:1985;774.
4. Carlsson J., Berzins H., Perlmann H., Perlmann P. Studies on Pf155/RESA and other soluble antigens from in vitro cultured Plasmodium falciparum. Parasitol. Res. 77:1991;27.
5. Cohen R.S., Blomberg F., Berzins K., Siekevitz P. The structure of post synaptic densities isolated from dog cerebral cortex: I. Overall morphology and protein composition. J. Cell Biol. 74:1977;181.
6. Coppel R.L., Cowman A.F., Anders R.F., Bianco A.E., Saint R.B., Lingelbach K.R., Kemp D.J., Brown G.V. Immune sera recognize on erythrocytes a Plasmodium falciparum antigen composed of repeated amino acid sequences. Nature. 310:1984;789.
7. Fievet, N., 1996. Modifications des réponses immunitaires à des antigènes des stades sanguins asexués de Plasmodium falciparum au cours de la grossesse et chez le nouveau-né au Cameroun. Ph.D. Thesis, University René Descartes, Paris, France.
8. Gabrielsen A.A. Jr., Jensen J.B., Boland M.T. Identification and partial characterization of exoantigens derived from medium used to culture Plasmodium falciparum. Am. J. Trop. Med. Hyg. 32:1983;671.
9. Jepsen S., Andersen B.J. Immunoadsorbent isolation of antigens from the culture media of in vitro cultivated Plasmodium falciparum. Acta Pathol. Microbiol. Scand., Section C. 89:1981;99.
10. Jepsen S., Hindersson P., Axelsen N.H. Computer controlled affinity chromatographical purification of soluble Plasmodium falciparum antigens from supernatants of in vitro cultures. Trans. R. Soc. Trop. Med. Hyg. 80:1986;534.
11. Laemmli U.K. Cleavage of the structural proteins during assembly of the head of bacteriophage T4. Nature. 227:1970;680.
12. Neville D.M. Jr. Molecular weight determination of protein-dodecyl sulfate complexes by gel electrophoresis in a discontinuous buffer system. J. Biol. Chem. 246:1971;6328.
13. Perlmann H., Berzins H., Wahlgren M., Carlsson J., Bjorkmann A., Patarroyo M.E., Perlmann P. Antibodies in malarial sera to parasite antigen in the membrane of erythrocytes infected with early asexual stages of Plasmodium falciparum. J. Exp. Med. 159:1984;1686.
14. Rodriguez da Silva L., Loche M., Dayal R., Perrin L.H. Plasmodium falciparum polypeptides released during in vitro cultivation. Bull. Who. 61:1983;105.
15. Trager W., Jensen J. Human malaria parasite in continuous culture. Science. 193:1976;673.
16. Tsang V.C.W., Peralta J.M., Ray Simons A. Enzyme-linked immunoelectrotransfer blot techniques (EITB) for studying the specificities of antigens and antibodies separated by gel electrophoresis. Methods Enzymol. 92:1983;377.
17. Udomsangpetch R., Lundgren K., Berzins K., Wahlin B., Perlmann H., Troye-Blomberg M., Carlsson J., Wahlgren M., Perlmann P., Bjorkman A. Human monoclonal antibodies to Pf155, a major antigen of the malaria parasite Plasmodium falciparum. Science. 231:1986;57.
18. Wahlgren, M., Berzins, K., Perlmann, H., Wahlin, B., Carlsson, J., Bjorkmann, A., Perlmann, P., McNicol, L.A., Dame, J.B., McCutchan, T.F., 1985. Pf155, a vaccine candidate for protection against asexual blood stages of the malaria parasite Plasmodium falciparum. In: Lerner, R.A., Chanock, R.M., Brown, F. (Eds.), Vaccines 85. Cold Spring Harbor Laboratory, New York, p. 51.