Cloning of a gene for chloroplast os desaturase of a green alga, Chlamydomonas reinhardtii

Journal of Biochemistry

Volumn 122, Issue 6, 1997, Pages 1224-1232

  Sato, Norihiro ^a   Fujiwara, Shoko ^a   Kawaguchi, Akihiko ^b   Tsuzuki, Mikio ^a  

^a TOKYO UNIVERSITY OF PHARMACY AND LIFE SCIENCES   (Japan)

^b UNIVERSITY OF TOKYO   (Japan)

Author keywords

Chlamydomonas reinhardtii; Complementation; Desaturation mutation; Heat stability; Photosystem II

Indexed keywords

ACYL COENZYME A DESATURASE; COMPLEMENTARY DNA; OLIGONUCLEOTIDE; POLYADENYLATED RNA;

ARTICLE; CHLAMYDOMONAS REINHARDTII; CHLOROPLAST; FATTY ACID DESATURATION; GREEN ALGA; MOLECULAR CLONING; NONHUMAN; OPEN READING FRAME; PHOTOSYNTHESIS; PHOTOSYSTEM I; PHOTOSYSTEM II; PLASTID; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SEQUENCE HOMOLOGY; THYLAKOID MEMBRANE;

ALGAE; CHLAMYDOMONAS REINHARDTII; CHLOROPHYTA; CYANOBACTERIA; EMBRYOPHYTA; EUKARYOTA;

EID: 0031460954     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.jbchem.a021885     Document Type: Article

References (36)

1. Roughan, P.G. and Slack, C.R. (1982) Cellular organization of glycerolipid metabolism. Annu. Rev. Plant Physiol. 33, 97-132
2. Browse, J. and Somerville, C. (1991) Glycerolipids synthesis: biochemistry and regulation. Annu. Rev. Plant Physiol. Plant Mol. Biol. 42, 467-506
3. 16:3 leaf lipids. Plant Physiol. 81, 859-864
4. Browse, J., Kunst, L., Anderson, S., Hugly, S., and Somerville, C.R. (1989) A mutant of Arabidopsis deficient in the chloroplast 16:1/18:1 desaturase. Plant Physiol. 90, 522-529
5. McConn, M. and Browse, J. (1996) The critical requirement for linolenic acid is pollen development, not photosynthesis, in an Arabidopsis mutant. Plant Cell 8, 403-416
6. Giroud, C., Gerber, A., and Eichenberger, W. (1988) Lipids of Chlamydomonas reinhardtii. Analysis of molecular species and intracellular site(s) of biosynthesis. Plant Cell Physiol. 29, 587-595
7. Sato, N., Tsuzuki, M., Matsuda, Y., Ehara, T., Osafune, T., and Kawaguchi, A. (1995) Isolation and characterization of mutants affected in lipid metabolism of Chlamydomonas reinhardtii. Eur. J. Biochem. 230, 987-993
8. Hitz, W.D., Carlson, T.J., Booth, J.R., Kinney, A.J., Stecca, K.L., and Yadav, N.S. (1994) Cloning of a higher plant plastid ω6 fatty acid desaturase cDNA and its expression in a cyanobacterium. Plant Physiol. 105, 635-641
9. Schmidt, H., Dresselhaus, T., Buck, F., and Heinz, E. (1994) Purification and PCR-based cDNA cloning of a plastidial n-6 desaturase. Plant Mol. Biol. 26, 631-642
10. Falcone, D.L., Gibson, S., Lemieux, B., and Somerville, C. (1994) Identification of a gene that complements an Arabidopsis mutant deficient in chloroplast ω6 desaturase activity. Plant Physiol. 106, 1453-1459
11. Wada, H., Gombos, Z., and Murata, N. (1990) Enhancement of chilling tolerance of a cyanobacterium by genetic manipulation of fatty acid desaturation. Nature 347, 200-203
12. Sakamoto, T., Wada, H., Nishida, I., Ohmori, M., and Murata, N. (1994) Identification of conserved domains in the Δ12 desaturases of cyanobacteria. Plant Mol. Biol. 24, 643-650
13. Kindle, K.L. (1990) High-frequency nuclear transformation of Chlamydomonas reinhardtii. Proc. Natl. Acad. Sci. USA 87, 1228-1232
14. Sueoka, N. (1960) Miotic replication of deoxyribonucleic acid replication in Chlamydomonas reinhardtii. Proc. Natl. Acad. Sci. USA 46, 83-91
15. Sambrook, J., Fritsch, E.F., and Maniatis, T. (1989) Molecular Cloning. A Laboratory Manual, Cold Spring Harbor Lab., Cold Spring Harbor, NY
16. Fujiwara, S., Sawada, M., and Someya, J. (1994) Molecular phylogenetic analysis of rbcL in the Prymnesiophyta. J. Phycol. 30, 863-871
17. Sato, N., Sonoike, K., Tsuzuki, M., and Kawaguchi, A (1995) Impaired photosystem II in a mutant of Chlamydomonas reinhardtii defective in sulfoquinovosyl diacylglycerol. Eur. J. Biochem. 234, 16-23
18. Bligh, E.G. and Dyer, W.J. (1959) A rapid method of total lipid extraction and purification. Can. J. Biochem. Physiol. 37, 911-917
19. 2 concentration during growth on fatty acid composition of microalgae. Plant Physiol. 93, 851-856
20. Shanklin, J., Whittle, E., and Fox, B.G. (1994) Eight histidine residues are catalytically essential in a membrane-associated iron enzyme, stearyl-CoA desaturase, and are conserved in alkane hydroxylase and xylene monooxygenase. Biochemistry 33, 12787-12794
21. Avelange-Macherel, M.H., Macherel, D., Wada, H., and Murata, N. (1995) Site-directed mutagenesis of histidine residues in the Δ12 acyl-lipid desaturase of Synechocystis. FEBS Lett. 361, 111-114
22. Sato, N., Sonoike, K., Tsuzuki, M., and Kawaguchi, A. (1996) Photosynthetic characterization of a mutant of Chlamydomonas reinhardtii impaired in fatty acid desaturation in chloroplasts. Biochim. Biophys. Acta 1274, 112-118
23. Sato, N., Sonoike, K., Kawaguchi, A., and Tsuzuki, M. (1996) Contribution of lowered unsaturation levels of chloroplast lipids to high temperature tolerance of photosynthesis in Chlamydomonas reinhardtii. J. Photochem. Photobiol. 36, 333-337
24. Schmidt, H. and Heinz, E. (1990) Desaturation of oleoyl groups in envelope membranes from spinach chloroplasts. Proc. Natl. Acad. Sci. USA 87, 9477-9480
25. von Heijne, G., Steppuhn, J., and Hermann, G. (1989) Domain structure of mitochondrial and chloroplast targeting peptides. Eur. J. Biochem. 180, 535-545
26. Franzen, L.G., Rochaix, J.D., and von Heijne, G. (1990) Chloroplast transit peptides from the green alga Chlamydomonas reinhardtii share features with both mitochondrial and higher plant chloroplast presequences. FEBS Lett. 260, 165-168
27. Gibson, S., Arondel, V., Iba, K., and Somerville, C. (1994) Cloning of a temperature-regulated gene encoding a chloroplast ω-3 desaturase from Arabidopsis thaliana. Plant Physiol. 106, 1615-1621
28. Heppard, E.P., Kinney, A.J., Stecca, K.L., and Miao, G.H. (1996) Developmental and growth temperature regulation of two different microsomal ω-6 desaturase genes in soybeans. Plant Physiol. 110, 311-319
29. Sodeinde, O.A. and Kindle, K.L. (1993) Homologous recombination in the nuclear genome of Chlamydomonas reinhardtii. Proc. Natl. Acad. Sci. USA 90, 9199-9203
30. Pearcy, R. (1978) Effects of growth temperature on the fatty acid composition of the leaf lipids in Atriplex lentiformis (Torr.) wats. Plant Physiol. 61, 484-486
31. Raison, J.K., Roberts, J.K.M., and Berry, J.A. (1978) Correlations between the thermal stability of chloroplast (thylakoid) membranes and the composition and fluidity of their polar lipids upon acclimation of the higher plant, Nerium oleander, to growth temperature. Biochim. Biophys. Acta 688, 218-228
32. Hugly, S., Kunst, L., Browse, J., and Sommerville, C. (1989) Enhanced thermal tolerance of photosynthesis and altered chloroplast ultrastructure in a mutant of Arabidopsis deficient in lipid desaturation. Plant Physiol. 90, 1134-1142
33. Thoma, P.G., Dominy, P.J., Vigh, L., Mansourian, A.R., Quinn, P.J., and Williams, W.P. (1986) Increased thermal stability of pigment-protein complexes of pea thylakoids following catalytic hydrogenation of membrane lipids. Biochim. Biophys. Acta 849, 131-140
34. Gombos, Z., Wada, H., and Murata, N. (1991) Direct evaluation of effects of fatty-acid unsaturation on the thermal properties of photosynthetic activities, as studied by mutation and transformation of Synechocystis PCC6803. Plant Cell Physiol. 32, 205-211
35. Los, D., Horvath, I., Vigh, L., and Murata, N. (1993) The temperature-dependent expression of the desaturase gene desA in Synechocystis PCC6803. FEBS Lett. 318, 57-60
36. Hamada, T., Nishiuchi, T., Kodama, H., Nishimura, M., and Iba, K. (1996) cDNA cloning of a wounding-inducible gene encoding a plastid ω-3 fatty acid desaturase from Tobacco. Plant Cell Physiol. 37, 606-611