An improved method for preparing lysozyme with chemically 13C-Enriched methionine residues using 2-aminothiophenol as a reagent of thiolysis

Journal of Biochemistry

Volumn 122, Issue 6, 1997, Pages 1153-1159

  Abe, Yoshito ^a,b   Ueda, Tadashi ^a   Imoto, Taiji ^a  

^a KYUSHU UNIVERSITY   (Japan)

^b BIOMOL ENG RESEARCH INSTITUTE   (Japan)

Author keywords

Chemical modification; Depression of deamidation; Isotope labeling; Lysozyme; NMR

Indexed keywords

2 AMINOTHIOPHENOL; CARBON 13; DITHIOTHREITOL; LYSOZYME; METHIONINE; MYOGLOBIN; PHENOL DERIVATIVE; UNCLASSIFIED DRUG;

ARTICLE; CHEMICAL LABELING; CHEMICAL MODIFICATION; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; CRYSTAL STRUCTURE; DEAMINATION; DEMETHYLATION; ENZYME SUBSTRATE; ISOTOPE LABELING; METHYLATION; NUCLEAR OVERHAUSER EFFECT; REDUCTION; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; STEREOCHEMISTRY;

EID: 0031459329     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.jbchem.a021875     Document Type: Article

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