Regulation of exogenous and endogenous glucose metabolism by insulin and acetoacetate in the isolated working rat heart. A three tracer study of glycolysis, glycogen metabolism, and glucose oxidation

Journal of Clinical Investigation

Volumn 100, Issue 11, 1997, Pages 2892-2899

  Russell III, Raymond R ^a   Cline, Gary W ^a   Guthrie, Patrick H ^b   Goodwin, Gary W ^b   Shulman, Gerald I ^a   Taegtmeyer, Heinrich ^b  

^a YALE UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b UNIVERSITY OF TEXAS MEDICAL SCHOOL   (United States)

Author keywords

Citric acid cycle; Isotopomer analysis; NMR

Indexed keywords

ACETOACETIC ACID;

ANIMAL TISSUE; ARTICLE; CITRIC ACID CYCLE; ENERGY METABOLISM; GLUCOSE METABOLISM; GLUCOSE OXIDATION; GLUCOSE TRANSPORT; HEART MUSCLE METABOLISM; HEART PERFORMANCE; HORMONAL REGULATION; INSULIN METABOLISM; ISOLATED HEART; METABOLIC REGULATION; NONHUMAN; NUCLEAR MAGNETIC RESONANCE; PRIORITY JOURNAL; RAT;

EID: 0031456506     PISSN: 00219738     EISSN: None     Source Type: Journal    
DOI: 10.1172/JCI119838     Document Type: Article

References (38)

1. Taegtmeyer, H. 1994. Energy metabolism of the heart: from basic concepts to clinical applications. Curr. Probl. Cardiol. 19:57-116.
2. Taegtmeyer, H., R. Hems, and H.A. Krebs. 1980. Utilization of energy providing substrates in the isolated working rat heart. Biochem. J. 186:701-711.
3. Goodwin, G.W., J.R. Arteaga, and H. Taegtmeyer. 1995. Glycogen turnover in the isolated working rat heart. J. Biol. Chem. 270:9234-9240.
4. Goodwin, G. W., F. Ahmad, and H. Taegtmeyer. 1996. Preferential oxidation of glycogen in isolated working rat heart. J. Clin. Invest. 97:1409-1416.
5. Henning, S.L., R.B. Wambolt, B.O. Schönekess, G.D. Lopaschuk, and M.F. Allard. 1996. Contribution of glycogen to aerobic myocardial glucose utilization. Circulation. 93:1549-1555.
6. Russell, R.R., V.T.B. Nguyêñ, J.M. Mrus, and H. Taegtmeyer. 1992. Fasting and lactate unmask insulin responsiveness in the isolated working rat heart. Am. J. Physiol. 263:E556-E561.
7. Kashiwaya, Y., K. Sato, N. Tsuchiya, S. Thomas, D.A. Fell, R.L. Veech, and J.V. Passonneau. 1994. Control of glucose utilization in working perfused rat heart. J. Biol. Chem. 269:25502-25514.
8. Russell, R.R, and H. Taegtmeyer. 1991. Changes in citric acid cycle flux and anaplerosis antedate the functional decline in isolated rat hearts utilizing acetoacetate. J. Clin. Invest. 87:384-390.
9. 13C NMR spectroscopy. FEBS Lett. 212:58-62.
10. 13C NMR spectroscopy. J. Biol. Chem. 263:6964-6971.
11. Leimer, K.R., R.H. Rice, and C.W. Gehrke. 1977. Complete mass spectra on N-trifluoroacetyl-n-butyl esters of amino acids. J. Chromatogr. 141:121-144.
12. Shulman, G.I., L. Rossetti, D.L. Rothman, J.B. Blair, and D. Smith. 1987. Quantitative analysis of glycogen repletion by nuclear magnetic resonance spectroscopy in the conscious rat. J. Clin. Invest. 80:387-393.
13. Weiss, R.G., V.P. Chacko, and G. Gerstenblith. 1989. Fatty acid regulation of glucose metabolism in the intact beating rat heart assessed by carbon-13 spectroscopy: the critical role of pyruvate dehydrogenase. J. Mol. Cell. Cardiol. 21:469-478.
14. Lewandowski, E.D., and L.T. White. 1995. Pyruvate dehydrogenase influences postischemic heart function. Circulation. 91:2071-2079.
15. 18F]2-deoxy-2-fluoro-D-glucose for assessing myocardial glucose uptake. Circulation. 91:2435-2444.
16. Katz, J., and A. Dunn. 1967. Glucose-2-t as a tracer for glucose metabolism. Biochemistry. 6:1-5.
17. Bergmeyer, H.U. 1974. Methods of Enzymatic Analysis. Verlag-Chemie International, Deerfield Beach, FL. 2000 pp.
18. 18F-2-deoxy-2-fluoro-D-glucose. Am. J. Physiol. 259: H1022-H1031.
19. Williamson, J.R., and H.A. Krebs. 1961. Acetoacetate as fuel of respiration in the perfused rat heart. Biochem. J. 80:540-547.
20. Depré, C., K. Veitch, and L. Hue. 1993. Role of fructose 2,6-bisphosphate in the control of glycolysis. Stimulation of glycogen synthesis by lactate in the isolated working rat heart. Acta Cardiol. 48:147-164.
21. Laughlin, M.R., J. Taylor, A.S. Chesnick, and R.S. Balaban. 1994. Non-glucose substrates increase glycogen synthesis in vivo in dog heart. Am. J. Physiol. 267:H217-H223.
22. Bolukoglu, H., G.W. Goodwin, P.H. Guthrie, S.G. Carmical, T.M. Chen, and H. Taegtmeyer. 1996. Metabolic fate of glucose in reversible low-flow ischemia of the isolated working rat heart. Am. J. Physiol. 270:H817-H826.
23. McNulty, P.H., A.J. Sinusas, C.Q.X. Shi, D. Dione, L.H. Young, G.C. Cline, and G.I. Shulman. 1996. Glucose metabolism distal to a critical coronary stenosis in a canine model of low-flow myocardial ischemia. J. Clin. Invest. 98: 62-69.
24. Brainard, J.R., J. Y. Hutson, D.E. Hoekenga, and R. Lenhoff. 1989. Ordered synthesis and mobilization of glycogen in the perfused heart. Biochemistry. 28:9766-9772.
25. Cohen, P. 1986. Muscle glycogen synthase. In The Enzymes. P.D. Boyer and E.G. Krebs, editors. Academic Press Limited, London. 461-497.
26. Garland, P.B., P.J. Randle, and E.A. Newsholme. 1963. Citrate as an intermediary in the inhibition of phosphofructokinase in rat heart muscle by fatty acids, ketone bodies, pyruvate, diabetes and starvation. Nature. 200: 169-170.
27. Villar-Palasi, C. 1995. Effect of glucose phosphorylation on the activation by insulin of skeletal muscle glycogen synthase. Biochim. Biophys. Acta. 1244:203-208.
28. Ebeling, P., and V.A. Koivisto. 1994. Non-esterified fatty acids regulate lipid and glucose oxidation and glycogen synthesis in healthy man. Diabetologia. 37:202-209.
29. Kelley, D.E., M. Mokan, J.A. Simoneau, and L.J. Mandarine. 1993. Interaction between glucose and free fatty acid metabolism in human skeletal muscle. J. Clin. Invest. 92:91-98.
30. Olson, M.S., S.C. Dennis, M.S. DeBuysere, and A. Padma. 1978. The regulation of pyruvate dehydrogenase in the isolated perfused rat heart. J. Biol. Chem. 253:7369-7375.
31. Little, J.R., M. Goto, and J.J. Spitzer. 1971. Effect of ketones on metabolism of free fatty acids by dog myocardium and skeletal muscle in vivo. Am. J. Physiol. 219:1458-1463.
32. Olson, R.E. 1962. Effect of pyruvate and acetoacelate on the metabolism of fatty acids by the perfused rat heart. Nature. 195:597-599.
33. Jeffrey, F.M., V. Diczku, A.D. Sherry, and C.R. Malloy. 1995. Substrate selection in the isolated working rat heart: effects of reperfusion, afterload, and concentration. Basic Res. Cardiol. 90:388-396.
34. Neely, J.R., M.J. Rovetto, and J.F. Oram. 1972. Myocardial utilization of carbohydrates and lipids. Prog. Cardiovasc. Dis. 15:289-329.
35. Saddik, M., and G.D. Lopaschuk. 1991. Myocardial triglyceride turnover and contribution to energy substrate utilisation in isolated working rat hearts. J. Biol. Chem. 236:8162-8170.
36. Latipää, P.M., K.J. Peuhkurinen, J.K. Hiltunen, and I.E. Hassinen. 1985. Regulation of pyruvate dehydrogenase during infusion of fatty acids of varying chain lengths in the perfused rat heart. J. Mol. Clin. Cardiol. 17:1161-1171.
37. Schönekess, B.O., P.G. Brindley, and G.D. Lopaschuk. 1995. Calcium regulation of glycolysis, glucose oxidation, and fatty acid oxidation in the aerobic and ischemic heart. Can. J. Physiol. Pharmacol. 73:1632-1640.
38. 13C]pyruvate. FEBS Lett. 303:247-250.