Crystallization and preliminary X-ray studies of two serine proteinase inhibitors, BGIA and BGIT from the seeds of bitter gourd

Journal of Structural Biology

Volumn 120, Issue 2, 1997, Pages 204-206

  Kondo, Hidemasa ^a   Nakanose, Jin ^a   Nakagawa, Atsushi ^a   Tanaka, Isao ^a   Kimura, Makoto ^b   Funatsu, Gunki ^b  

^a HOKKAIDO UNIVERSITY   (Japan)

^b KYUSHU UNIVERSITY   (Japan)

Author keywords

Crystallization; Proteinase inhibitor

Indexed keywords

COLOCYNTH; SERINE PROTEINASE INHIBITOR;

ARTICLE; CRYSTAL STRUCTURE; DRUG CONFORMATION; DRUG ISOLATION; NONHUMAN; PLANT SEED; PRIORITY JOURNAL; PROTEINASE INHIBITION; STREPTOMYCES GRISEUS; STRUCTURE ANALYSIS; X RAY DIFFRACTION;

CITRULLUS COLOCYNTHIS; CUCURBITA; MOMORDICA CHARANTIA; STREPTOMYCES GRISEUS;

EID: 0031456307     PISSN: 10478477     EISSN: None     Source Type: Journal    
DOI: 10.1006/jsbi.1997.3931     Document Type: Article

References (13)

1. Blow D. M., Janin J., Sweet R. M. Mode of action of soybean trypsin inhibitor (Kunitz) as a model for specific protein-protein interactions. Nature. 249:1974;54-57.
2. Bode W., Greyling H. J., Huber R., Otlewski J., Wilusz T. The refined 2.0 Å X-ray crystal structure of the complex formed between bovine beta-trypsin and CMTI-1, a trypsin inhibitor from squash seeds (Cucurbita maxima). Topological similarity of the squash seed inhibitors with the carboxypeptidase A inhibitor from potatoes. FEBS Lett. 242:1989;285-292.
3. Bode W., Huber R. Natural protein proteinase inhibitors and their interaction with proteinases. Eur. J. Biochem. 204:1992;433-451.
4. Holak T. A., Bode W., Huber R., Otlewski J., Wilusz T. Nuclear magnetic resonance solution and X-ray structures of squash trypsin inhibitor exhibit the same conformation of the proteinase binding loop. J. Mol. Biol. 210:1989;649-654.
5. Ishino Y., Ueno T., Miyagi M., Uemori T., Imamura M., Tsunasawa S., Kato I. Overproduction of Thermus aquaticus DNA polymerase and its structural analysis by ion-spray mass spectrometry. J. Biochem. 116:1994;1019-1024.
6. Liu J., Prakash O., Cai M., Gong Y., Huang Y., Wen L., Wen J. J., Huang J. K., Krishnamoorthi R. Solution structure and backbone dynamics of recombinantCucurbita maxima. Biochemistry. 35:1996;1516-1524.
7. Matthews B. W. Solvent content of protein crystals. J. Mol. Biol. 33:1968;491-497.
8. McPherson A. Current approaches to macromolecular crystallization. Eur. J. Biochem. 101:1990;387-396.
9. Miura S., Funatsu G. Isolation and amino acid sequence of two trypsin inhibitors from the seeds of bitter gourd (Momordica charantia. Biosci. Biotech. Biochem. 59:1995;496-473.
10. Miyahara J., Takahashi K., Amemiya Y., Kamiya N., Satow Y. A new type of X-ray area detector utilizing laser stimulated luminescence. Nucl. Instrum. Methods. A246:1985;572-578.
11. Ogata F., Miyata T., Fujii N., Yoshida N., Noda K., Makisumi S., Ito A. Purification and amino acid sequence of a bitter gourd inhibitor against an acidic amino acid-specific endopeptidase ofStreptomyces griseus. J. Biol. Chem. 226:1991;16715-16721.
12. Z. Otwinowski, 1993, Proceeding of the CCP4 Study Weekend, 56, 62, Science and Engineering Research Council, Daresbury Laboratory, Daresbury, UK.
13. Sakabe N., Ikemizu S., Sakabe K., Higashi T., Nakagawa A., Watanabe N., Adachi S., Sasaki K. Wessinberg camera for macromolecules with imaging plate data collection system at the Photon Factory: Present status and future plan. Rev. Sci. Instrum. 66:1995;1276-1281.