Overexpression of Rab3D enhances regulated amylase secretion from pancreatic acini of transgenic mice

Journal of Clinical Investigation

Volumn 100, Issue 12, 1997, Pages 3044-3052

  Ohnishi, Hirohide ^a   Samuelson, Linda C ^a   Yule, David I ^a   Ernst, Stephen A ^a   Williams, John A ^a  

^a UNIVERSITY OF MICHIGAN MEDICAL SCHOOL   (United States)

Author keywords

GTP binding protein; Hemagglutinin tag; Pancreatic zymogen granules; Permeabilized acini; Regulated exocytosis

Indexed keywords

CALCIUM; GUANINE NUCLEOTIDE BINDING PROTEIN; ZYMOGEN GRANULE;

ACINAR CELL; AMYLASE RELEASE; ARTICLE; ENZYME REGULATION; EXOCYTOSIS; HORMONAL REGULATION; PRIORITY JOURNAL; REGULATORY MECHANISM;

EID: 0031454368     PISSN: 00219738     EISSN: None     Source Type: Journal    
DOI: 10.1172/JCI119859     Document Type: Article

References (44)

1. Südhof, T. 1995. The synaptic vesicle cycle: a cascade of protein-protein interactions. Nature. 375:645-653.
2. Wagner, A.C.C., and J.A. Williams. 1994. Pancreatic zymogen granule membrane proteins: molecular details begin to emerge. Digestion. 55:191-199.
3. E: a GTP-binding protein mediating exocytosis. Annu. Rev. Physiol. 52:591-606.
4. Balch, W.E. 1990. Small GTP-binding proteins in vesicular transport. Trends Biochem. Sci. 15:473-477.
5. Simons, K., and M. Zerial. 1993. Rab proteins and the road maps for intracellular transport. Neuron. 11:789-799.
6. Novick, P., and P. Brennwald. 1993. Friends and family: the role of the Rab GTPases in vesicular traffic. Cell. 75:597-601.
7. Fischer von Mollard, G., B. Stahl, C. Li, T.C. Südhof, and R. Jahn. 1994. Rab proteins in regulated exocytosis. Trends Biochem. Sci. 19:164-168.
8. Johannes, L., P.M. Lledo, M. Roa, J.D. Vincent, J. P. Henry, and F. Darchen. 1994. The GTPase Rab3a negatively controls calcium-dependent exocytosis in neuroendoerine cells. EMBO (Eur. Mol. Biol. Organ.) J. 13:2029-2037.
9. 2+-dependent exocytosis from adrenal chromaffin cells. J. Biol. Chem. 269:10229-10234.
10. Fischer von Mollard, G., B. Stahl, A. Khokhlaychev, T.C. Sudhof, and R. Jahn. 1994. Rab3C is a synaptic vesicle protein that dissociates from synaptic vesicles after stimulation of exocytosis. J. Biol. Chem. 269:10971-10974.
11. 2+-dependent exocytosis in rat anterior pituitary cells. Nature. 364:540-544.
12. Weber, E., T. Jilling, and K.L. Kirk. 1996. Distinct functional properties of Rab3A and Rab3B in PC12 neuroendocrine cells. J. Biol. Chem. 271:6963-6971.
13. Baldini, G., T. Hohl, H.Y. Lin, and H.F. Lodish. 1992. Cloning of a Rab3 isotype predominantly expressed in adipocytes. Proc. Natl. Acad. Sci. USA. 89:5049-5052.
14. Ohnishi, H., S.A. Ernst, N. Wys, M. McNiven, and J.A. Williams. 1996. Rab3D localized to zymogen granules in rat pancreatic acini and other exocrine glands. Am. J. Physiol. 271:G531-G538.
15. Valentijn, J.A., D. Sengupta, F.D. Gumkowski, L.H. Tang, E.M. Konieczko, and J.D. Jamieson. 1996. Rab3D localized to secretory granules in rat pancreatic acinar cells. Eur. J. Cell Biol. 70:33-41.
16. Tang, L.H., F.D. Gumkowski, D. Sengupta, I.M. Modlin, and J.D. Jamieson. 1996. Rab3D protein is a specific marker for zymogen granules in gastric chief cells of rats and rabbits. Gastroenterology. 110:809-820.
17. Raffaniello, R.D., J. Lin, F. Wang, and J.-P. Raufman. 1996. Expression of Rab3D in dispersed chief cells from guinea pig stomach. Biochim. Biophys. Acta. 1311:111-116.
18. Padfield, P.J., W.E. Balch, and J.D. Jamieson. 1992. A synthetic peptide of the Rab3a effector domain stimulates amylase release from permeabilized pancreratic acini. Proc. Natl. Acad. Sci. USA. 89:1656-1660.
19. Wagner, A.C.C., M.Z. Strowski, and J.A. Williams. 1994. Identification of Rab5 hut not Rab3A in rat pancreatic zymogen granule membranes. Biochem. Biophys. Res. Commun. 200:542-548.
20. Law, G.J., A.J. Northrop, and W.T. Mason. 1993. Rab3-peptide stimulates exocytosis from mast cells via a pertussis toxin-sensitive mechanism. FEBS Lett. 33:56-60.
21. Ullrich, O., H. Horiuchi, C. Bucci, and M. Zerial. 1994. Membrane association of Rab5 mediated by GDP-dissociation inhibitor and accompanied by GDP/GTP exchange. Nature. 368:157-160.
22. Chen, Y.T., C. Holcomb, and H.P.H. Moore. 1993. Expression and localization of two low molecular weight GTP-binding proteins, Rah8 and Rab10, by epitope tag. Proc. Natl. Acad. Sci. USA. 90:6508-6512.
23. Hogan, B., R. Beddington, F. Constantini, and E. Lacy. 1994. Manipulating the Mouse Embryo: A Laboratory Manual. Cold Spring Harbor Laboratory Press. Cold Spring Harbor, New York.
24. Miller, S.A., D.D. Dykes, and H.F. Poleshy. 1988. A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Res. 16:1215.
25. Laemmli, U.K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227:680-685.
26. Wagner, A.C.C., M.J. Wishart, S.M. Mulders, P.M. Blevins, P.C. Andrews, A.W. Lowe, and J.A. Williams. 1994. GP-3, a newly characterized glycoprotein on the inner surface of the zymogen granule membrane, undergoes regulated secretion. J. Biol. Chem. 269:9099-9104.
27. Burnham, D.B., and J.A. Williams. 1982. Effects of carbachol, cholecystokinin, and insulin on protein phosphorylation in isolated pancreatic acini. J. Biol. Chem. 257:10523-10528.
28. Kitagawa, M., J.A. Williams, and R.C. De Lisle. 1990. Amylase release from streptolysin O-permeabilized pancreatic acini. Am. J. Physiol. 259:G157-G164.
29. ras in fibroblasts. Mol. Cell. Biol. 13:1903-1910.
30. Brondyk, W.H., C.J. McKiernan, E.S. Burstein, and I.G. Macara. 1993. Mutants of Rab3A analogous to oncogenic ras mutants. J. Biol. Chem. 268: 9410-9415.
31. Burstein, E.S., W.H. Brondyk, I.G. Macara, K. Kaibuchi, and Y. Takai. 1993. Regulation of the GTPase cycle of the neuronally expressed ras-like GTP-binding protein Rab3A. J. Biol. Chem. 268:22247-22250.
32. 2+-dependent amylase secretion from pancreatic acinar cells occurs without activation of phospholipase C linked G-proteins. Biochem. Biophys. Res. Commun. 174:536-541.
33. 2+-dependent amylase secretion from SLO-permeabilized rat pancreatic acini requires diffusible cytosolic proteins. Am. J. Physiol. 269:G647-G652.
34. Ornitz. D.M., R.D. Palmiter, R.E. Hammer, R.L. Brinster, G.H. Swift, and R.J. MacDonald. 1985. Specific expression of an elastase-human growth hormone fusion gene in pancreatic acinar cells of transgenic mice. Nature. 313: 600-602.
35. Muallem, S., K. Kwiatkowska, X. Xu, and H.L. Yin. 1995. Actin filament disassembly is a sufficient final trigger for exocytosis in nonexcitable cells. J. Cell Biol. 128:589-598.
36. Valentijn, J.A., F.D. Gumkowski, and J.D. Jamieson. 1996. The expression pattern of Rab3D in the developing rat exocrine pancreas coincides with the acquisition of regulated exocytosis. Eur. J. Cell Biol. 71:129-136.
37. Shirataki, H., K. Kaibuchi, Y. Sakoda, S. Kishida, T. Yamaguchi, K. Wada, M. Miyazaki, and Y. Takai. 1993. Rabphilin-3A, a putative target protein for smg p25A/rab3A p25 small GTP-binding protein related to synaptotagmin. Mol. Cell. Biol. 13:2061-2068.
38. Chung, S.H., Y. Takai, and R.W. Holz. 1995. Evidence that the Rab3a-binding protein, rabphilin3a, enhances regulated secretion. J. Biol. Chem. 270: 16714-16718.
39. Søgaard, M., K. Tani, R.R. Ye, S. Geromanos, P. Tempst, T. Kirchhausen, J.E. Rothman, and T. Söllner. 1994. A Rab protein is required for the assembly of SNARE complexes in the docking of transport vesicles. Cell. 78: 937-948.
40. Gaisano, H.Y., M. Ghai, P.N. Malkus, L. Sheu, A. Bouquillon, M.K. Bennett, and W.S. Trimble. 1996. Distinct cellular locations of the syntaxin family of proteins in rat pancreatic acinar cells. Mol. Biol. Cell. 7:2019-2027.
41. Gaisano, H.Y., L. Sheu, G. Grondin, M. Ghai, A. Bouquillon, A. Lowe, A. Beaudoin, and W.S. Trimble. 1996. The vesicle-associated membrane protein family of proteins in rat pancreatic and parotid acinar cells. Gastroenterology. 111:1661-1669.
42. Walworth, N.C., P. Brennwald, A.K. Kabcenell, M. Garrett, and P. Novick. 1992. Hydrolysis of GTP by Sec4 protein plays an important role in vesicular transport and is stimulated by a GTPase-activating protein in Saccharomyces cerevisiae. Mol. Cell. Biol. 12:2017-2028.
43. Stahl, B., G. Fischer von Mollard, C.W. Solimena, and R. Jahn. 1994. GTP cleavage by the small GTP-binding protein RAb3A is associated with exocytosis of synaptic vesicles induced by α-latrotoxin. J. Biol. Chem. 269:24770-24776.
44. Duan, R.-D., C.-F. Zheng, K.-L. Guan, and J.A. Williams. 1995. Activation of MAP kinase kinase (MEK) and Ras by cholecystokinin in rat pancreatic acini. Am. J. Physiol. 268:G1060-G1065.