Cytokine mRNA expression in CSF and peripheral blood mononuclear cells in multiple sclerosis: Detection by RT-PCR without in vitro stimulation

Journal of Neuroimmunology

Volumn 80, Issue 1-2, 1997, Pages 137-142

  Monteyne, Philippe ^a   Van Laere, Valérie ^a   Marichal, Roseline ^a   Sindic, Christian J M ^a  

^a UNIVERSITÉ CATHOLIQUE DE LOUVAIN   (Belgium)

Author keywords

Cerebrospinal fluid; Cytokines; Multiple sclerosis

Indexed keywords

CYTOKINE; GAMMA INTERFERON; INTERLEUKIN 10; TUMOR NECROSIS FACTOR ALPHA;

ADULT; ARTICLE; CEREBROSPINAL FLUID; CLINICAL ARTICLE; DISEASE ACTIVITY; FEMALE; GENE AMPLIFICATION; HUMAN; HUMAN CELL; HYBRIDIZATION; IMMUNOREACTIVITY; IMMUNOREGULATION; INFLAMMATION; MALE; MONONUCLEAR CELL; MULTIPLE SCLEROSIS; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

ADULT; AGED; CYTOKINES; FEMALE; HUMANS; INTERFERON TYPE II; INTERLEUKIN-10; LEUKOCYTES, MONONUCLEAR; LYMPHOCYTE ACTIVATION; MALE; MIDDLE AGED; MULTIPLE SCLEROSIS; POLYMERASE CHAIN REACTION; RNA, MESSENGER; TUMOR NECROSIS FACTOR-ALPHA;

EID: 0031453594     PISSN: 01655728     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0165-5728(97)00148-3     Document Type: Article

References (15)

1. Beck J., Rondot P., Catinot L., Falcoff E., Kirchner H., Wietzerbin J. Increased production of interferon gamma and tumor necrosis factor precedes clinical manifestation in multiple sclerosis: Do cytokine trigger off exacerbations? Acta Neurol. Scand. 78:1988;318-323.
2. Bell J.I., Lathorp G.M. Multiple loci for multiple sclerosis. Nat. Genet. 13:1996;377-378.
3. Biliau A. Interferon-γ: Biology and role in pathogenesis. Adv. Immunol. 62:1996;61-130.
4. Chofflon M., Juillard C., Juillard P., Gauthier G., Grau G.E. Tumor necrosis factor alpha production as a possible predictor of relapse in patients with multiple sclerosis. Eur. Cytokine Netw. 3:1992;523-531.
5. Hartung H.P. Immune-mediated demyelination. Ann. Neurol. 33:1993;563-567.
6. Link J., Söderström M., Olsson T., Höjeberg B., Ljungdahl A., Link H. Increased transforming growth factor-β, interleukin-4 and interferon-γ in multiple sclerosis. Ann. Neurol. 36:1994;379-386.
7. Navikas V., Link J., Palasik W., Söderström M., Fredrikson S., Olsson T., Link H. Increased mRNA expression of IL-10 in mononuclear cells in multiple sclerosis and optic neuritis. Scand. J. Immunol. 41:1995;171-178.
8. Navikas V., Haglund M., Link J., He B., Lindqvist L., Fredrikson S., Link H. Cytokine mRNA profiles in mononuclear cells in acute aseptic meningoencephalitis. Infect. Immun. 63:1995;1581-1586.
9. Navikas V., He B., Link J., Haglund M., Soderstrom M., Fredrikson S., Ljungdahl A., Hojeberg J., Qiao J., Olsson T., Link H. Augmented expression of tumour necrosis factor-alpha and lymphotoxin in mononuclear cells in multiple sclerosis and optic neuritis. Brain. 119:1996;213-223.
10. Panitch H.S., Bever C.T. Clinical trials of interferons in multiple sclerosis. What we have learned? J. Neuroimmunol. 46:1993;155-164.
11. Poser C.M., Paty D.W., Scheinberg L., McDonald W.I., Davis F.A., Ebers G.C., Johnson K.P., Sibley W.A., Silberberg D.H., Tourtelotte W.W. New diagnostic criteria for multiple sclerosis: Guidelines for research protocols. Ann. Neurol. 13:1983;227-231.
12. Rieckmann P., Albrecht M., Kitze B., Weber T., Tumani H., Broocks A., Luër W., Poser S. Cytokine mRNA levels in mononuclear blood cells from patients with multiple sclerosis. Neurology. 44:1994;1523-1526.
13. Rieckmann P., Albrecht M., Kitze B., Weber T., Tumani H., Broocks A., Luër W., Helwig A., Poser S. Tumor necrosis factor-α messenger RNA expression in patients with relapsing-remitting multiple sclerosis is associated with disease activity. Ann. Neurol. 37:1995;82-88.
14. Rieckmann P., Albrecht M., Ehrenreich H., Weber T., Michel U. Semi-quantitative analysis of cytokine gene expression in blood and cerebrospinal fluid cells by reverse transcriptase polymerase chain reaction. Res. Exp. Med. 195:1995;17-29.
15. Sharief M.K., Hentges R. Association between tumor necrosis factor-α and disease progression in patients with multiple sclerosis. New Engl. J. Med. 325:1991;467-472.