A method for recovery of Candida albicans DNA from larger blood samples and its detection by polymerase chain reaction on proteinase genes

Mycoses

Volumn 40, Issue 7-8, 1997, Pages 249-253

  Reichard, U ^a   Margraf, S ^a   Hube, B ^b   Ruchel R ^a  

^a UNIVERSITY OF GÖTTINGEN   (Germany)

^b UNIVERSITY OF HAMBURG   (Germany)

Author keywords

Aspartic proteinase; Candida albicans; Diagnosis; PCR; Polymerase chain reaction; 1,3 glucanase

Indexed keywords

ASPARTIC PROTEINASE; PROTEINASE;

ACCURACY; ARTICLE; BLOOD ANALYSIS; CANDIDA ALBICANS; CANDIDIASIS; CONTROLLED STUDY; FUNGUS IDENTIFICATION; HUMAN; HUMAN TISSUE; MYCOSIS; NORMAL HUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; TECHNIQUE;

EID: 0031452328     PISSN: 09337407     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1439-0507.1997.tb00228.x     Document Type: Article

References (18)

1. Walsh, T. J., Franccsconi, A., Kasai, M. & Chanock, S. J. (1995) PCR and single-strand conformational polymorphism for recognition of medically important opportunistic fungi. J. Clin. Microbiol. 33, 3216-3220.
2. Schönian, G., Tietz, H.-J., Thanos, M. & Gräser, Y. (1996) Application of molecularbiological techniques for diagnosis and epidemiology of fungal infections in humans. mycoses 39 (Suppl. 1), 73-80.
3. Wildfeuer, A., Schlenk, R. & Friedrich, W. (1996) Detection of Candida albicans DNA with a yeast-specific primer system by polymerase chain reaction. mycoses 39, 341-346.
4. Rand, K. H., Houck, H. & Wolff, H. (1994) Detection of candidemia by polymerase chain reaction. Mol. Cell. Probes 8, 215-221.
5. Buchman, T. G., Rossier, M., Merz, W. G. & Charache, P. (1990) Detection of surgical pathogens by in vitro DNA amplification. Part I. Rapid identification of Candida albicans by in vitro amplification of a fungus-specific gene. Surgery 108, 338-347.
6. Hube, B., Turver, C. J., Odds, F. C., Eiffert, H., Boulnois, G. J., Köchel, H. & Rüchel, R. (1990) Sequence of the Candida albicans gene encoding the secretory aspartate proteinase. J. Med. Vet. Mycol. 29, 129-132.
7. Wright, R. J., Carne, A., Hieber, A. D., Lamont, I. L., Emerson, G. W. & Sullivan, P. A. (1992) A second gene for a secreted aspartate proteinase in Candida albicans. J. Bact. 174, 7848-7853.
8. Rüchel, R. (1991) Proteinase. In: Bennett, J.E., Hay, R.J. & Peterson, P.K. (eds.) New strategies in fungal disease. Proceedings of an international Symposium. Brocket Hall, Hetfordshire in Sept. 1991. Churchill-Livingstone, pp. 17-33.
9. Monod, M., Togni, G., Hube, B. & Sanglard, D. (1994) Multiplicity of genes encoding secreted aspartic protein-ases in Candida species. Mol. Microbiol. 13, 357-368.
10. Sugita, Y., Kanaizuka, I., Nakajima, H., Ibe, M., Yokota, S. & Matsuyama, S. (1993) Detection of Candida albicans DNA in cerebrospinal fluid. J. Med. Vet. Mycol. 31, 353-358.
11. Sambrook, J., Fritsch, E. F. & Maniatis, T. (1989) Molecular Cloning: a laboratory Manual, 2nd edn., Cold Spring Harbor, NY: Cold Spring Harbour Laboratory.
12. Burgener-Kairuz, P., Zuber, J. P., Jaunin, P., Buchman, T. G., Bille, J. & Rossier, M. (1994) Rapid detection and identification of Candida albicans and Torulopsis (Candida) glabrata in clinical specimens by species-specific nested PCR amplification of a cytochrome P-450 lanosterol-alpha-demethylase (L1A1) gene fragment. J. Clin. Microbiol. 32, 1902-1907.
13. Telenti, A. & Roberts, G. D. (1989) Fungal blood cultures. Eur. J. Clin. Microbiol. Infect. Dis. 8, 825-831.
14. Rüchel, R., de Bernardis, F., Ray, T. L., Sullivan, P. A. & Cole, G. T. (1992) Candida acid proteinases. J. Med. Vet. Mycol. 30 (Suppl. 1), 123-132.
15. Davies, D. R. (1990) The structure and function of the aspartic proteinases. Ann. Rev. Biophys. Chem. 19, 189-215.
16. Hube, B., Sanglard, D., Odds, F. C., Hess, D., Monod, M., Schäfer, W., Brown, A. J. P. & Gow, N. A. R. (1997) Disruption of each of the secreted aspartyl proteinase genes SAP1, SAP2, and SAP3 of Candida albicans attenuates virulence. Infect. Immun. 65, 3529-3538.
17. White, T. C., Miyasaki, S. H. & Agabian, N. (1993) Three distinct secreted aspartyl proteinases in Candida albicans. J. Bacteriol. 175, 6126-6133.
18. Miyasaki, S. H., White, T. C. & Agabian, N. (1994) A fourth secreted aspartyl proteinase gene (SAP4) and a CARE2 repetitive element are located upstream of the SAP1 gene in Candida albicans. J. Bacteriol. 176, 1702-1710.