Inhibitory effects of human α2-macroglobulin on Trypanosoma cruzi epimastigote proteinases

Acta Tropica

Volumn 68, Issue 3, 1997, Pages 327-337

  Ramos, Adrián ^a   Remedi, M Sara ^a   Sánchez, Cecilia ^a   Bonacci, Gustavo ^a   Vides, Miguel A ^a   Chiabrando, Gustavo ^a  

^a UNIVERSIDAD NACIONAL DE CÓRDOBA   (Argentina)

Author keywords

Alpha 2 macroglobulin; Proteinases; Trypanosoma cruzi

Indexed keywords

ALPHA 2 MACROGLOBULIN; PROTEINASE;

ANIMAL EXPERIMENT; ARTICLE; CONTROLLED STUDY; ENZYME INACTIVATION; EPIMASTIGOTE; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN DEGRADATION; TRYPANOSOMA CRUZI;

ALPHA-MACROGLOBULINS; ANIMALS; DOSE-RESPONSE RELATIONSHIP, DRUG; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENDOPEPTIDASES; LEUPEPTINS; PROTEASE INHIBITORS; TIME FACTORS; TOSYL COMPOUNDS; TRYPANOSOMA CRUZI;

EID: 0031447167     PISSN: 0001706X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0001-706X(97)00110-1     Document Type: Article

References (28)

1. Araújo-Jorge T., Barbosa H., Meirelles M. Trypanosoma cruzi recognition by macrophages and muscle cells: Perspectives after a 15-year study. Mem. Inst. Oswaldo Cruz, Río de Janeiro. 87(Suppl. 5):1992;43-56.
2. Athauda S., Ido E., Arakawa H., Nishigai M., Kyushiki H., Yoshinaka Y., Takahashi T., Ikai A., Tang J., Takahashi K. Entrapment and inhibition of human immunodeficiency virus proteinase by α2-macroglobulin and structural changes in the inhibitor. J. Biochem. 113:1993;742-746.
3. Barret A.J., Starkey P.M. The interaction of α2-macrogtobulin with proteinases. Biochem. J. 133:1973;709-724.
4. Barret A.J., Brown M.A., Sayers C.A. The electrophoretically 'Slow' and 'Fast' forms of the α2-macroglobulin molecule. Biochem. J. 181:1979;401-418.
5. Bloom H., Beier H., Gross H.S. Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels. Electrophoresis. 8:1987;93-99.
6. Bonaldo M.C., d'Escoffier L.N., Salles J.M., Goldemberg S. Characterization and expression of proteases during Trypanosoma cruzi metacyclogenesis. Exp. Parasitol. 73:1991;44-51.
7. Bongertz V., Hungerer K.D. Trypanosoma cruzi isolation and characterization of a protease. Exp. Parasitol. 45:1978;8-18.
8. Bontempi E., Franke de Cazzulo B.M., Ruiz A.M., Cazzulo J.J. Purification and some properties of an acidic protease of Trypanosoma cruzi. Comp. Biochem. Physiol. 77B:1984;599-604.
9. Bradford M.M. Rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:1976;544-552.
10. Calderón R.O., Luján H.D., Aguerri A.M., Bronia D.H. Trypanosoma cruzi: Involvement of proteolytic activity during cell fusion induced by epimastigote form. Mol. Cell. Biochem. 86:1989;189-200.
11. Campetella O., Martínez J., Cazzulo J.J. A major proteinase is developmentally regulated in Trypanosoma cruzi. FEMS Microbiol. Lett. 67:1990;145-150.
12. Cazzulo J.J., Cazzulo Franke M.C., Martínez J., Franke de Cazzulo B.M. Some kinetic properties of a cysteine proteinase (cruzipain) from Trypanosoma cruzi. Biochim. Biophys. Acta. 1037:1990;186-191.
13. Cazzulo J.J., Franke de Cazzulo B.M., Engel J.C., Cannata J.J. End products and enzyme levels of aerobic glucose fermentation in trypanosomatids. Mol. Biochem. Parasitol. 16:1985;329-343.
14. Chiabrando G., Bonacci G., Sánchez C., Ramos A., Zalazar F., Vides M.A. A procedure for human pregnancy zone protein (and human α2-macroglobulin) purification using Hydrophobic interaction chromatography on phenyl-sepharose CL-4B column. Protein Expr. Purif. 9:1997;399-406.
15. Eakin A.E., Mills A.A., Harth G., McKerrow J.H., Craik C.S. The sequence, organization, and expression of the major cysteine protease (cruzain) from Trypanosoma cruzi. J. Biol. Chem. 267:1992;7411-7420.
16. Heumann D., Burger D., Vischer T., de Colmenares M., Bouvier J., Bordier C. Molecular interactions of Leishmania promastigote surface protease with human α2-macroglobulin. Mol. Biochem. Parasitol. 33:1989;67-72.
17. Jensen P.E.H., Stigbrand T. Differences in the proteinase inhibition mechanism of human α2-macroglobulin and pregnancy zone protein. Eur. J. Biochem. 21O:1992;1071-1077.
18. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227:1970;680-685.
19. Korzus E., Luisetti M., Travis J. Interactions of α1-antichymotrypsin, α1-proteinase inhibitor, and α2-macroglobulin with the fungal enzyme, Seaprose. Biol. Chem. Hoppe-Seyler. 375:1994;335-341.
20. McKerrow J.H. The proteases and pathogenicity of parasitic protozoa. Annu. Rev. Microbiol. 44:1993;821-853.
21. Miyoshi S., Shinoda S. Inhibitory effect of α2-Macroglobulin on Vibrio vulnificus protease. J. Biochem. 106:1989;299-303.
22. Piras M.M., Henríquez D., Piras R. The effect of proteolytic enzymes and protease inhibitors on the interaction Trypanosoma cruzi fibroblasts. Mol. Biochem. Parasitol. 14:1985;151-163.
23. Rangel H.A., Araújo P.M.F., Repka D., Costa M.G. Trypanosoma cruzi: Isolation and characterization of a proteinase. Exp. Parasitol. 52:1981;199-209.
24. Sand O., Folkersen J., Westergaard J.G., Sottrup-Jensen L. Characterization of human pregnancy zone protein. J. Biol. Chem. 26O:1985;15723-15735.
25. Scharfstein J., Schechter M., Senna M., Peralta J.M., Mendonca-Previato L., Milés M.A. Trypanosoma cruzi: Characterization and isolation of a 57/51,000 M.W. surface glycoprotein (GP57/51) expressed by epimastigotes and bloodstream trypomastigotes. J. Immunol. 137:1986;1336-1341.
26. Stoka V., Nycander M., Lenarcic B., Labriola C., Cazzulo J.J., Biork I., Turk V. Inhibition of cruzipain, the major cysteine proteinase of the protozoan parasite, Trypanosoma cruzi, by proteinase inhibitors of the cystatin superfamily. FEBS Lett. 370:1995;101-104.
27. Van Leuven F., Marynen P., Cassiman J.-J., Van den Berghe H. Receptormediated endocytosis of α2-macroglobulin-protease complexes by fibroblasts in culture. FEBS Lett. 134:1981;83-87.
28. Wang D., Yuan A.I., Feinman R.D. Covalent thrombin-α2-macroglobulin complexes. Evidence for bivalent cross-linking of inhibitor chains by a single enzyme molecule. Biochemistry. 23:1984;2807-2811.