Expression and mutagenesis of the dpsA gene of Synechococcus sp. PCC7942, encoding a DNA-binding protein involved in oxidative stress protection

FEMS Microbiology Letters

Volumn 155, Issue 1, 1997, Pages 85-91

  Dwivedi, Kshitij ^a   Sen, Aparna ^a   Bullerjahn, George S ^a  

^a Center for Photochemical Sciences   (United States)

Author keywords

Dps protein; Oxidative stress; Peroxide resistance; RpoS

Indexed keywords

DNA BINDING PROTEIN; PEROXIDE;

ANTIOXIDANT ACTIVITY; ARTICLE; GRAM NEGATIVE BACTERIUM; NONHUMAN; OXIDATIVE STRESS; PHOTOOXIDATION; PRIORITY JOURNAL; PROTEIN DNA BINDING;

SYNECHOCOCCUS;

EID: 0031446577     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(97)00371-6     Document Type: Article

References (18)

1. Almiron, M., Link, A.J., Furlong, D. and Kolter, R. (1992) A novel DNA-binding protein with regulatory and protective roles in starved Escherichia coli. Genes Dev. 6, 2646-2654.
2. S in stationary phase. Mol. Microbiol. 13, 265-272.
3. 38-dependent expression of a core Escherichia coli starvation gene, pexB. J. Bacteriol. 176, 3928-3935.
4. Peña, M.M.O., Burkhart, W. and Bullerjahn, G.S. (1995) Purification and characterization of a Synechococcus sp. strain PCC7942 polypeptide structurally similar to the stress-induced Dps/PexB protein of Escherichia coli. Arch. Microbiol. 163, 337-344.
5. Chen, L. and Hellmann, J.D. (1995) Bacillus subtilis MrgA is a Dps(PexB) homolog: evidence for metalloregulation of an oxidative stress gene. Mol. Microbiol. 18, 295-300.
6. Peña, M.M.O. and Bullerjahn, G.S. (1995) The DpsA protein of Synechococcus sp. strain PCC7942 is a DNA-binding hemoprotein: linkage of the Dps and bacterioferritin protein families. J. Biol. Chem. 270, 22478-22482.
7. Grossman, M.J., Hinton, S.M., Minak-Bernero, V., Slaughter, C. and Stiefel, E.I. (1992) Unification of the ferritin family of proteins. Proc. Natl. Acad. Sci. USA 89, 2419-2423.
8. Pessolani, M.C.V., Smith, D.R., Riviore, B., McCormick, J., Hefta, S.A., Cole, S.T. and Brennan, P.J. (1994) Purification, characterization, gene sequence and significance of a bacterioferritin from Mycobacterium leprae. J. Exp. Med. 180, 319-327.
9. Allen, M.M. (1968) Simple conditions for growth of unicellular blue-green algae on plates. J. Phycol. 4, 1-4.
10. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning, A Laboratory Manual, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
11. Sanger, F.S., Nicklen, S. and Coulson, A.R. (1977) DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74, 5463-5467.
12. Golden, S.S. and Sherman, L.A. (1984) Optimal conditions for genetic transformation of the cyanobacterium Anacystis nidulans R2. J. Bacteriol. 158, 36-42.
13. Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685.
14. Towbin, H., Staehelin, T. and Gordon, J. (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. USA 76, 4350-4354.
15. Hiratsu, K., Shinagawa, H. and Makino, K. (1995) Mode of promoter recognition by the Escherichia coli RNA polymerase holoenzyme containing the sigma S subunit: identification of the recognition site of the fic promoter. Mol. Microbiol. 18, 841-850.
16. S. J. Bacteriol. 178, 2785-2793.
17. S promoters. Mol. Microbiol. 21, 657-659.
18. Chen, L., James, L.P. and Helmann, J.D. (1993) Metalloregulation in Bacillus subtilis: isolation and characterization of two genes differentially repressed by metal ions. J. Bacteriol. 175, 5428-5437.