Single-lens theta microscopy - A new implementation of confocal theta microscopy

Journal of Microscopy

Volumn 188, Issue 3, 1997, Pages 280-284

  Lindek, S ^a   Stefany, T ^a   Stelzer, E H K ^a  

^a EUROPEAN MOLECULAR BIOLOGY LABORATORY   (Germany)

Author keywords

4Pi microscopy; Confocal; Fluorescence; Numerical aperture; Resolution; Theta microscopy; Water immersion objective lenses

Indexed keywords

OPTICAL INSTRUMENT LENSES;

4PI MICROSCOPY; CONFOCAL; IMMERSION OBJECTIVE LENS; NUMERICAL APERTURE; OBJECTIVE LENS; RESOLUTION; SINGLE OBJECTIVE; THETA MICROSCOPY; WATER IMMERSION; WATER-IMMERSION OBJECTIVE LENS;

MIRRORS;

ARTICLE; CONFOCAL MICROSCOPY; ILLUMINATION; INSTRUMENT; LENS; PRIORITY JOURNAL;

EID: 0031446356     PISSN: 00222720     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2818.1997.2650822.x     Document Type: Article

References (10)

1. Ashkin, A. & Dziedzic, J.M. (1987) Optical trapping and manipulation of viruses and bacteria. Science, 235, 1517-1520.
2. Ashkin, A., Dziedzic, J.M. & Yamanc, T. (1987) Optical trapping and manipulation of single cells using infrared laser beams. Nature, 330, 769-771.
3. Dixon, A.E. & Cogswell, C. (1995) Confocal microscopy with transmitted light. Handbook of Biological Confocal Microscopy (ed. by J. B. Pawley), pp. 479-490. Plenum Press, New York.
4. Hell, S. & Stelzer, E.H.K. (1992) Properties of a 4Pi confocal fluorescence microscope. J. Opt. Soc. Am. A, 9, 2159-2166.
5. Lindek, S., Cremer, C. & Stelzer, E.H.K. (1996) Confocal theta fluorescence microscopy with annular apertures. Appl. Opt. 35, 126-130.
6. Lindek, S., Pick, R. & Stelzer, E.H.K. (1994a) Confocal theta microscope with three objective lenses. Rev. Sci. Instrum. 65, 3367-3372.
7. Lindek, S., Salmon, N., Cremer, C. & Stelzer, E.H.K. (1994b) Theta microscopy allows phase regulation in 4Pi(A)-confocal two-photon fluorescence microscopy. Optik. 98, 15-20.
8. McCutchen, C.W. (1967) Superresolution in microscopy and the Abbe resolution limit. J. Opt. Soc. Am. 57, 1190-1192.
9. Stelzer, E.H.K. (1995) The intermediate optical system of laser-scanning confocal microscopes. Handbook of Biological Confocal Microscopy (ed. by J. B. Pawley). pp. 139-154. Plenum Press, New York.
10. Stelzer, E.H.K. & Lindek, S. (1994) Fundamental reduction of the observation volume in far-field light microscopy by detection orthogonal to the illumination axis: confocal theta microscopy. Opt. Commun. 111, 536-547.