Lipase-based quantitation of triacylglycerols in cellular lipid extracts: Requirement for presence of detergent and prior separation by thin- layer chromatography

Lipids

Volumn 32, Issue 12, 1997, Pages 1297-1300

  Van Veldhoven, Paul P ^a   Swinnen, Johannes V ^a   Esquenet, Murielle ^a   Verhoeven, Guido ^a  

^a UNIVERSITY OF LEUVEN   (Belgium)

Author keywords

[No Author keywords available]

Indexed keywords

CELL EXTRACT; DETERGENT; TRIACYLGLYCEROL;

ARTICLE; CELL LEVEL; CONTROLLED STUDY; HUMAN; HUMAN CELL; LIPID ANALYSIS; LIPID COMPOSITION; THIN LAYER CHROMATOGRAPHY;

PSEUDOMONAS; PSEUDOMONAS SP.;

EID: 0031445663     PISSN: 00244201     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11745-006-0166-1     Document Type: Article

References (10)

1. Swinnen, J.V., Van Veldhoven, P.P., Esquenet, M., Heyns, W., and Verhoeven, G. (1996) Androgens Markedly Stimulate the Accumulation of Neutral Lipids in the Human Prostatic Adenocarcinoma Cell Line LNCaP, Endocrinology 137, 4468-4474.
2. Fossatu, P., and Prencipe, L. (1982) Serum Triglycerides Determined Colorimetrically with an Enzyme That Produces Hydrogen Peroxide, Clin. Chem. 28, 2077-2080.
3. McGowan, M.W., Artiss, J.D., Strandbergh, D.R., and Zak, B. (1983) A Peroxidase-Coupled Method for the Colorimetric Determination of Serum Triglycerides, Clin. Chem. 29, 538-542.
4. Nägele, U., Hägele, E.O., Sauer, G., Wiedermann, E., Lehmann, P., Wahlefeld, A.W., and Gruber, W. (1984) Reagent for the Enzymatic Determination of Serum Total Triglycerides with Improved Lipolytic Efficiency, J. Clin. Chem. Clin. Biochem. 22, 165-174.
5. Van Veldhoven, P.P., and Bell, R.M. (1988) Effect of Harvesting Methods, Growth Conditions and Growth Phase on Diacylglycerol Levels in Cultured Human Adherent Cells, Biochim. Biophys. Acta 959, 185-196.
6. Van Veldhoven, P.P., Croes, K., Casteels, M., and Mannaerts, G.P. (1997) 2-Methylacyl-CoA Racemase: A Coupled Assay Based on the Use of Pristanoyl-CoA Oxidase/Peroxidase and Reinvestigation of Its Subcellular Distribution in Rat and Human Liver, Biochim. Biophys. Acta 1347, 62-68.
7. Van Veldhoven, P.P. (1995) Activity Measurements of Acyl-CoA Oxidases in Human Liver, J. Inher. Metab. Dis. 18 (Suppl. 1), 125-134.
8. Nägele, U., Wahlefeld, A.-W., and Ziegenhorn, J. (1983) Triglycerides - Colorimetric Method, Methods in Enzymatic Analysis (3rd Edition) VIII, 1-12, VCN, Weinheim, Germany.
9. Trinder, P., and Webster, D. (1984) Determination of HDL-Cholesterol Using 2,4,6-Tribromo-3-Hydroxybenzoic Acid with a Commercial CHOD-PAP Reacent, Ann. Clin. Biochem. 21, 430-433.
10. Guilbault, G., Kramer, D.N., and Hackley, E. (1967) New Substrate for Fluorimetric Determination of Oxidative Enzymes, Anal. Chem. 39, 271.