Hormonal regulation of cholesterol 7α-hydroxylase specific activity, mRNA levels, and transcriptional activity in vivo in the rat

Journal of Lipid Research

Volumn 38, Issue 12, 1997, Pages 2483-2491

  Pandak, W M ^a   Heuman, D M ^a   Redford, K ^a   Stravitz, R T ^a   Chiang, J Y L ^c   Hylemon, P B ^b   Vlahcevic, Z R ^a  

^a VIRGINIA COMMONWEALTH UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b VIRGINIA COMMONWEALTH UNIVERSITY   (United States)

^c NORTHEAST OHIO MEDICAL UNIVERSITY   (United States)

Author keywords

Cholesterol; Cholesterol 7 hydroxylase; Gene; Glucocorticoid; Liver; Regulation; Thyroid

Indexed keywords

CHOLESTEROL; CHOLESTEROL 7ALPHA MONOOXYGENASE; CORTICOSTERONE; GLUCOCORTICOID; MESSENGER RNA; THYROID HORMONE;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; BILE ACID SYNTHESIS; CHOLESTEROL METABOLISM; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME INDUCTION; GENETIC TRANSCRIPTION; HORMONAL REGULATION; HYPOPHYSIS ADRENAL SYSTEM; HYPOPHYSIS THYROID SYSTEM; LIVER CELL; MALE; MESSENGER RNA SYNTHESIS; NONHUMAN; PRIORITY JOURNAL; RAT;

ADRENALECTOMY; ANIMALS; BODY WEIGHT; CHOLESTEROL; CHOLESTEROL 7-ALPHA-HYDROXYLASE; CORTICOSTERONE; GENE EXPRESSION REGULATION, ENZYMOLOGIC; HYDROXYMETHYLGLUTARYL COA REDUCTASES; HYPOPHYSECTOMY; LIVER; MALE; RATS; RATS, SPRAGUE-DAWLEY; RNA, MESSENGER; THYROID HORMONES; THYROIDECTOMY; TRANSCRIPTION, GENETIC; TRIGLYCERIDES;

EID: 0031444340     PISSN: 00222275     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (47)

1. Vlahcevic, Z. R., W. M. Pandak, D. M. Heuman, and P. B. Hylemon. 1992. Function and regulation of hydroxylases involved in the bile acid biosynthesis pathways. Semin. Liver Dis. 12: 403-419.
2. Russell, D. W., and K. D. Setchell. 1992. Bile acid biosynthesis. Biochemistry. 31: 4737-4749.
3. Stravitz, R. T., Z. R. Vlahcevic, T. L. Russell, M. L. Heizer, N. C. Avadhani, and P. B. Hylemon. 1996. Regulation of sterol 27-hydroxylase and an alternative pathway of bile acid biosynthesis in primary cultures of rat hepatocytes. J. Steroid Biochem. & Mol. Biol. 57: 337-347.
4. Princen, H. M. G., P. Meijer, and B. Hofstee. 1989. Dexamethasone regulates bile acid synthesis in monolayer cultures of rat hepatocytes by induction of cholesterol 7α-hydroxylase. Biochem. J. 262: 341-348.
5. Vlahcevic, Z. R., D. M. Heuman, R. T. Stravitz, P. B. Hylemon, and W. M. Pandak. 1997. Qualitative estimate of contribution of bile acid synthetic pathway to total bile acid synthesis in the rat. Gastroenterology. In press.
6. Pandak, W. M., Y. C. Li, J. Y. L. Chiang, E. J. Studer, E. C. Gurley, D. M. Heuman, Z. R. Vlahcevic, and P. B. Hylemon. 1991. Regulation of cholesterol 7α-hydroxylase mRNA and transcriptional activity by taurocholate and cholesterol in the chronic biliary diverted rat. J. Biol. Chem. 266: 3416-3421.
7. Pandak, W. M., Z. R. Vlahcevic, D. M. Heuman, K. S. Redford, J. Y. L. Chiang, and P. B. Hylemon. 1994. Effects of different bile salts on steady-state mRNA levels and transcriptional activity of cholesterol 7α-hydroxylase. Hepatology. 19: 941-947.
8. Stravitz, R. T., P. B. Hylemon, D. M. Heuman, L. R. Hagey, C. D. Schteingart, H. T. Ton-Nu, A. F. Hofmann, and Z. R. Vlahcevic. 1993. Transcriptional regulation of cholesterol 7α-hydroxylase mRNA by conjugated bile acids in primary cultures of rat hepatocytes. J. Biol. Chem. 268: 13987-13993.
9. Twisk, J., E. M. Lehmann, and H. M. G. Princen. 1993. Differential feedback regulation of cholesterol 7α-hydroxylase mRNA and transcriptional activity by rat bile acids in primary monolayer cultures of rat hepatocytes. Biochem. J. 290: 685-691.
10. Hoekman, M. F. M., J. M. J. Rientjes, J. Twisk, R. J. Planta, H. M. G. Princen, and W. H. Mager. 1993. Transcriptional regulation of the gene encoding cholesterol 7α-hydroxylase in the rat. Gene. 180: 217-223.
11. Jones, M. P., W. M. Pandak, D. M. Heuman, J. Y. L. Chiang, P. B. Hylemon, and Z. R. Vlahcevic. 1993. Cholesterol 7α-hydroxylase: evidence for transcriptional regulation by cholesterol or metabolic products of cholesterol in the rat. J. Lipid Res. 34: 885-892.
12. Doerner, K. C., E. C. Gurley, Z. R. Vlahcevic, and P. B. Hylemon. 1995. Regulation of cholesterol 7α-hydroxylase and HMG-CoA reductase by squalestatin in primary cultures of adult rat hepatocytes. J. Lipid Res. 36: 1168-1177.
13. Stravitz, R. T., Z. R. Vlahcevic, E. C. Gurley, and P. B. Hylemon. 1995. Repression of cholesterol 7α-hydroxylase transcription by bile acid is mediated through protein kinase C in primary cultured rat hepatocytes. J. Lipid Res. 36: 1359-1369.
14. Stravitz, R. T., Y. P. Raao, Z. R. Vlahcevic, E. C. Gurley, D. W. Jarvis, and P. B. Hylemon. 1996. Hepatocellular protein kinase C activation by bile acids: implications for regulation of cholesterol 7α-hydroxylase. Am. J. Physiol. 34: G293-G303.
15. Vlahcevic, Z. R., S. K. Jairath, R. T. Stravitz, D. M. Heuman, P. B. Hylemon, N. G. Avadhani, and W. M. Pandak. 1996. Transcriptional regulation of hepatic sterol 27-hydroxylase by bile acids. Am. J. Physiol. 270: G646-G652.
16. Princen, H. M. G., P. Meijer, and B. Hofstee. 1989. Dexamethasone regulates bile acid synthesis in monolayer cultures of rat hepatocytes by induction of cholesterol 7α-hydroxylase. Biochem. J. 262: 341-348.
17. Hylemon, P. B., E. C. Gurley, R. T. Stravitz, J. S. Litz, W. M. Pandak, J. Y. L. Chiang, and Z. R. Vlahcevic. 1992. Hormonal regulation of cholesterol 7α-hydroxylase mRNA levels and transcriptional activity in primary rat hepatocyte cultures. J. Biol. Chem. 267: 16866-16871.
18. Ness, G. C., L. C. Pendleton, Y. C. Li, and J. Y. L. Chiang. 1990. Effect of thyroid hormone on hepatic cholesterol 7α-hydroxylase, LDL receptor, HMG-CoA reductase, farnesyl pyrophosphate synthetase, and apolipoprotein A-I mRNA levels in hypophysectomized rats. Biochem. Biophys. Res. Commun. 172: 1150-1156.
19. Twisk, J., M. F. M. Hoekman, E. M. Lehmann, P. Meijer, W. H. Mager, and H. M. G. Princen. 1995. Insulin suppresses bile acid synthesis in cultured rat hepatocytes by down-regulation of cholesterol 7α-hydroxylase and sterol 27 hydroxylase gene transcription. Hepatology. 21: 501-510.
20. Pandak, W. M., D. M. Heuman, G. Compton, P. B. Hylemon, and Z. R. Vlahcevic. 1995. Regulation of hepatic cholesterol and bile acid biosynthesis by glucagon and enteroglucagon. Gastroenterology. 108: A1142.
21. Kubaska, W. M., E. C. Gurley, P. B. Hylemon, P. Guzelian, J. Gustafsson, and Z. R. Vlahcevic. 1985. Absence of negative feedback control of bile acid biosynthesis in cultured rat hepatocytes. J. Biol. Chem. 260: 13459-13463.
22. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye-binding. Anal. Biochem. 72: 248-254.
23. Whitehead, T. R., Z. R. Vlahcevic, Z. H. Beg, and P. B. Hylemon. 1984. Characterization of active and inactive forms of rat hepatic 3-hydroxy-3-methylglutaryl coenzyme A reductase. Arch. Biochem. Biophys. 230: 483-491.
24. Hylemon, P. B., E. J. Studer, W. M. Pandak, D. M. Heuman, Z. R. Vlahcevic, and J. Y. L. Chiang. 1989. Improved HPLC assay for measuring cholesterol 7α-hydroxylase activity. Anal. Biochem. 1820: 212-216.
25. Abell, L. L., B. B. Levy, B. B. Brodie, and F. E. Kendall. 1952. Simplified method for estimation of total cholesterol in serum and demonstration of its specificity. J. Biol. Chem. 195: 357-366.
26. Danielson, P. E., S. Forss-Petter, A. Brow, L. Calavetta, J. Douglass, R. J. Milner, and J. G. Sutcliffe. 1988. p1B15: a cDNA clone of the rat mRNA encoding cyclophilin. DNA. 7: 261-267.
27. Stravitz, R. T., Z. R. Vlahcevic, T. L. Russell, M. L. Heizer, N. G. Avadhani, and P. B. Hylemon. 1996. Regulation of sterol 27-hydroxylase and an alternative pathway of bile acid biosynthesis in primary cultures of rat hepatocytes. J. Steroid Biochem. & Mol. Biol. 57: 337-347.
28. Heubi, J. A., S. Burstein, M. A. Sperling, D. Gregg, M. T. R. Subbiah, and D. E. Matthews. 1983. The role of human growth hormone in the regulation of cholesterol and bile acid metabolism. J. Clin. Endocrinol. Metab. 57: 885-891.
29. Crestani, M., D. Stroup, and J. Y. L. Chiang. 1995. Hormonal regulation of the cholesterol 7α-hydroxylase gene (CYP7). J Lipid Res. 36: 2419-2432.
30. Pandak, W. M., R. T. Stravitz, V. Lucas, D. M. Heuman, and J. Y. L. Chiang. 1996. HepG2 cells: a model for studies on regulation of human cholesterol 7α-hydroxylase at the molecular level. Am. J. Physiol. 33: G401-G410.
31. Gebhard, R. L., and W. F. Prigge. 1992. Thyroid hormone differentially augments biliary sterol secretion in the rat. II. The chronic bile fistula model. J. Lipid Res. 33: 1467-1473.
32. Gielen, J., J. Van Cantfort, J. Robaye, and J. Renson. 1969. Rythme circadien de la cholesterol-7-α-hydroxylase chez la rat. C. R. Acad. Sci. Paris. 269: 731-732.
33. Balasubramaniam, S., K. A. Mitropoulos, and N. B. Myant. 1975. Hormonal control of the activities of cholesterol-7α-hydroxylase and hydroxymethyl-glutaryl-CoA reductase in rats. In Advances in Bile Acid Research. III. Bile Acid Meeting, Frieburg i Br. S. Matern, J. Hackenschmidt, P. Back, and W. Gerok, editors. 61-67.
34. Chiang, J. Y. L., W. F. Miller, and G-M. Lin. 1990. Regulation of cholesterol 7α-hydroxylase in the liver. Purification of cholesterol 7α-hydroxylase and the immunochemical evidence for the induction of cholesterol 7α-hydroxylase by cholestyramine and circadian rhythm. J. Biol. Chem. 265: 3889-3897.
35. Noshiro, M., M. Nishimoto, and K. Okuda. 1990. Rat liver cholesterol 7α-hydroxylase. Pretranslational regulation for circadian rhythm. J. Biol. Chem. 265: 10036-10041.
36. Lavery, D. J., and U. Schibler. 1993. Circadian transcription of the cholesterol 7α-hydroxylase gene may involve the liver-enriched bZIP protein DBP. Genes Dev. 7: 1871-1884.
37. Lee, Y. H., J. A. Alberta, F. J. Gonzalez, and D. J. Waxman. 1994. Multiple, functional DBP sites on the promoter of the cholesterol 7α-hydroxylase P450 gene, CYP7. J. Biol. Chem. 269: 14681-14689.
38. Tsai, M. J., and B. O'Mally. 1994. Synergism between different cis-acting elements. Annu. Rev. Biochem. 63: 451-486.
39. Oliviero, S., and K. Struhl. 1991. Synergistic transcriptional enhancement does not depend on the number of acidic activation domains bound to the promoter. Proc. Natl. Acad. Sci. USA. 88: 224-228.
40. Lin, Y. S., M. Carey, M. Ptashne, and M. R. Green. 1990. Different eukaryotic transcriptional activitors can cooperate promiscuously. Nature. 345: 359-361.
41. Goldstein, J. L., and M. S. Brown. 1990. Regulation of the mevalonate pathway. Nature. 343: 425-430.
42. Leers, J., C. Steiner, R. Renkawitz, and M. Muller. 1994. A thyroid hormone receptor dependent glucocorticoid induction. Mol. Endocrinol. 8: 440-447.
43. Guillemin, R., W. E. Dear, and R. A. Liebelt. 1959. Nyctohemeral variations in plasma free corticosteroid levels of the rat. Proc. Soc. Exp. Biol. Med. 101: 394-395.
44. Crestani, M., G. Galli, and J. Y. L. Chiang. 1993. Genomic cloning, sequencing, and analysis of the hamster cholesterol 7α-hydroxylase gene (CYP7). Arch. Biochem. Biophys. 306: 451-460.
45. Stravitz, R. T., Z. R. Vlahcevic, W. M. Pandak, A. Stolz, and P. B. Hylemon. 1994. Regulation of rat hepatic 3α-hydroxysteroid dehydrogenase in vivo and in primary cultures of rat hepatocytes. J. Lipid Res. 35: 239-247.
46. Brock, M. L., and D. J. Shapiro. 1983. Estrogen stabilizes vitellogenin mRNA against cytoplasmic degradation. Cell. 34: 207-214.
47. Fulton, J., and G. D. Birnie. 1985. Post-transcriptional regulation of rat liver expression by glucocorticoids. Nucleic Acids Res. 13: 6467-6482.