Simple dot-blot method evaluated for detection of antibodies against extractable nuclear antigens

Clinical Chemistry

Volumn 43, Issue 12, 1997, Pages 2420-2422

  Ermens, Anthony A M ^a,c   Bayens, Angelique J M ^a   Van Gemert, Anita C M ^a   Van Duijnhoven, Johannes L P ^b  

^a Diaconessenhuis   (Netherlands)

^b ELKERLIEK HOSPITAL   (Netherlands)

^c Diaconessenhuis   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBODY; CELL NUCLEUS ANTIGEN;

ANTIGEN ANTIBODY COMPLEX; ARTICLE; COUNTER IMMUNOELECTROPHORESIS; DOT HYBRIDIZATION; ENZYME LINKED IMMUNOSORBENT ASSAY; HUMAN; IMMUNOBLOTTING; IMMUNOFLUORESCENCE; SJOEGREN SYNDROME;

EID: 0031440261     PISSN: 00099147     EISSN: None     Source Type: Journal    
DOI: 10.1093/clinchem/43.12.2420     Document Type: Article

References (7)

1. Von Muhlen CA, Tan EM. Autoantibodies in the diagnosis of systemic rheumatic diseases. Semin Arthritis Rheum 1995;24:323-58.
2. Delpech A, Gilbert D, Daliphard S, le Loet X, Godin M, Tron F. Antibodies to Sm, RNP and SSB detected by solid-phase ELISAs using recombinant antigens: a comparison study with counter immunoelectrophoresis and immunoblotting. J Clin Lab Anal 1993;7:197-202.
3. Jaskowski TD, Schroder C, Martins TB, Mouritsen L, Hill HR. Comparison of three commercially available enzyme immunoassays for the screening of autoantibodies to extractable nuclear antigens. J Clin Lab Anal 1995;9: 166-72.
4. James K, Meek G. Evaluation of commercial enzyme immunoassays compared to immunofluorescence and double diffusion for autoantibodies associated with autoimmune disease. Am J Clin Pathol 1992;97:559-65.
5. Bunn C, Kveder T. Counterimmunoelectrophoresis and immunodiffusion for the detection of antibodies to soluble antigens. In: van Venrooij WJ, Maini RN, eds. Manual of biological markers of disease. Dordrecht, The Netherlands: Kluwer Academic Publishers, 1993;A3:1-12.
6. Verheijen R, Salden M, van Venrooij WJ. Protein blotting. In: van Venrooij WJ, Maini RN, eds. Manual of biological markers of disease. Dordrecht, The Netherlands: Kluwer Academic Publishers, 1993;A4:1-25.
7. Paxton H, Bendele T, O'Connor L, Haynes DC. Evaluation of the rheumastrip ANA profile test: a rapid test strip procedure for simultaneously determining antibodies to autoantigens U1-ribonucleoprotein (U1-RNP), Sm, SS-A/Ro, SS-B/La, and to native DNA. Clin Chem 1990;36:792-7.