Purification and functional reconstitution of the human CHIP28 water channel expressed in Saccharomyces cerevisiae

Protein Expression and Purification

Volumn 11, Issue 3, 1997, Pages 284-288

  Laizé, Vincent ^a   Ripoche, Pierre ^a   Tacnet, Frédérique ^a  

^a CEA SACLAY   (France)

Author keywords

[No Author keywords available]

Indexed keywords

INFLUENZA VIRUS; SACCHAROMYCES CEREVISIAE;

EID: 0031439976     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.1997.0798     Document Type: Article

References (17)

1. Preston G. M., Carroll T. P., Guggino W. B., Agre P. Appearance of water channels in Xenopus oocytes expressing red cell CHIP28 protein. Science. 256:1992;385-387.
2. Gorin M. B., Yancey S. B., Cline J., Revel J. P., Horwitz J. The major intrinsic protein (MIP) of the bovine lens fiber membrane: Characterization and structure based on cDNA cloning. Cell. 39:1984;49-59.
3. Laizé V., Rousselet G., Verbavatz J. M., Berthonaud V., Gobin R., Roudier N., Abrami L., Ripoche P., Tacnet F. Functional expression of the human CHIP28 water channel in a yeast secretory mutant. FEBS Lett. 373:1995;269-274.
4. Jung J. S., Preston G. M., Smith B. L., Guggino W. B., Agre P. Molecular structure of the water channel through aquaporin CHIP. The hourglass model. J. Biol. Chem. 269:1994;14648-14654.
5. Zeidel M. L., Ambudkar S. V., Smith B. L., Agre P. Reconstitution of functional water channels in liposomes containing purified red cell CHIP28 protein. Biochemistry. 31:1992;7436-7440.
6. Kolodziej P. A., Young R. A. Epitope tagging and protein surveillance. Methods Enzymol. 194:1991;508-519.
7. Walworth N. C., Novick P. J. Purification and characterization of constitutive secretory vesicles from yeast. J. Cell. Biol. 105:1987;163-174.
8. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:1976;248-254.
9. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227:1970;680-685.
10. Matsudaira P. Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes. J. Biol. Chem. 262:1987;10035-10038.
11. Rigaud J. L., Bluzat A., Buschlen S. Incorporation of bacteriorhodopsin into large unilamellar liposomes by reverse phase evaporation. Biochem. Biophys. Res. Commun. 111:1983;373-392.
12. Abrami L., Tacnet F., Ripoche P. Evidence for a glycerol pathway through aquaporin 1 (CHIP28) channels. Pflügers Arch. 430:1995;447-458.
13. Van Heeswijk M. P. E., Van Os C. H. Osmotic water permeabilities of brush border and basolateral membrane vesicles from rat renal cortex and small intestine. J. Membr. Biol. 92:1986;183-193.
14. Van Hoek A. N., Wiener M., Bicknese S., Miercke L., Biwersi J., Verkman A. S. Secondary structure analysis of purified functional CHIP28 water channels by CD and FTIR spectroscopy. Biochemistry. 32:1993;11847-11856.
15. Preston G. M., Jung J. S., Guggino W. B., Agre P. The mercury-sensitive residue at cysteine-189 in the CHIP28 water channel. J. Biol. Chem. 268:1993;17-20.
16. Zhang R., Van Hoek A. N., Biwersi J., Verkman A. S. A point mutation at cysteine 189 blocks the water permeability of rat kidney water channel CHIP28k. Biochemistry. 32:1993;2938-2941.
17. Sekler I., Kopito R., Casey J. R. High level expression, partial purification, and functional reconstitution of the human AE1 anion exchanger in Saccharomyces cerevisiae. J. Biol. Chem. 270:1995;21028-21034.