A new method to synthesize competitor RNAs for accurate analyses by competitive RT-PCR

Journal of Biochemical and Biophysical Methods

Volumn 35, Issue 3, 1997, Pages 203-207

  Ishibashi, Osamu ^a,b  

^a CIBA GEIGY LTD   (Switzerland)

^b NIIGATA UNIVERSITY   (Japan)

Author keywords

Competitive RT PCR; Long accurate PCR; Quantitative analyses; Reverse transcription

Indexed keywords

RNA;

ARTICLE; GENE AMPLIFICATION; HUMAN; MOLECULAR BIOLOGY; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA SYNTHESIS; STANDARD; SYNTHESIS;

POLYMERASE CHAIN REACTION; REFERENCE STANDARDS; RNA; RNA, MESSENGER; TEMPLATES, GENETIC; TRANSCRIPTION, GENETIC;

EID: 0031439867     PISSN: 0165022X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0165-022X(97)00036-5     Document Type: Article

References (5)

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2. Shuldiner A.R., DePablo F., Moore C.A., Roth J. Two nonallelic insulin genes in Xenopus laevis are expressed differentially during neurulation in prepancreatic embryos. Proc Natl Acad Sci USA. 88:1991;7679-7683.
3. Diviacco S., Norio P., Zentilin L., Menzo S., Clementi M., Biamonti G., Riva S., Falaschi A., Giacca M. A novel procedure for quantitative polymerase chain reaction by coamplification of competitive templates. Gene. 122:1992;313-320.
4. Celi F.S., Zenilman M.E., Shuldiner A.R. A rapid and versatile method to synthesize internal standards for competitive PCR. Nucleic Acids Res. 21:1993;1047.
5. Barnes W.M. PCR amplification of up to 35-kb DNA with high fidelity and high yield from λ bacteriophage templates. Proc Natl Acad Sci USA. 91:1994;2216-2220.