Human lymphocyte cytochrome P450 2E1, a putative marker for alcohol mediated changes in hepatic chlorzoxazone activity

Drug Metabolism and Disposition

Volumn 25, Issue 12, 1997, Pages 1429-1435

  Raucy, Judy L ^a   Schultz, Eric D ^b   Wester, Mike R ^b   Arora, Sanjeev ^b   Johnston, David E ^b   Omdahl, John L ^b   Carpenter, Susan P ^b  

^a AGOURON PHARMACEUTICALS INC   (United States)

^b NONE

Author keywords

[No Author keywords available]

Indexed keywords

ALCOHOL; CHLORZOXAZONE; CYTOCHROME P450;

ADULT; ALCOHOL ABUSE; ALCOHOL LIVER DISEASE; AREA UNDER THE CURVE; ARTICLE; CLINICAL ARTICLE; CONTROLLED STUDY; DNA SEQUENCE; DRUG BLOOD LEVEL; DRUG CLEARANCE; DRUG HYDROXYLATION; DRUG METABOLISM; ENZYME ACTIVITY; FEMALE; HUMAN; HUMAN CELL; IMMUNOBLOTTING; LIVER TOXICITY; LYMPHOCYTE; MALE; MICROSOME; ORAL DRUG ADMINISTRATION; PRIORITY JOURNAL; PROTEIN EXPRESSION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

ADULT; BIOLOGICAL MARKERS; CHLORZOXAZONE; CYTOCHROME P-450 CYP2E1; ETHANOL; FEMALE; HUMANS; LIVER; LYMPHOCYTES; MALE; MIDDLE AGED; RANDOM ALLOCATION;

EID: 0031437805     PISSN: 00909556     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (41)

1. D. R. Koop: Oxidative and reductive metabolism by cytochrome P4502E1. FASEB J. 6, 724-730 (1992).
2. J. L. Raucy, J. C. Kraner, and J. M. Lasker: Bioactivation of halogenated hydrocarbons by cytochrome P4502E1. Crit. Rev. Toxicol. 23, 1-20 (1993).
3. G. Ekstrom and M. Ingelman-Sundberg: Rat liver microsomal NADPH-supported oxidase activity and lipid peroxidation dependent on ethanol-inducible cytochrome P-450 (P-450IIE1). Biochem. Pharmacol. 38, 1313-1319 (1989).
4. T. Castillo, D. R. Koop, S. Kamimura, G. Triadafilopoulos, and H. Tsukamoto: Role of cytochrome P-450 2E1 in ethanol-, carbon tetrachloride- and iron-dependent microsomal lipid peroxidation. Hepatology 16, 992-996 (1992).
5. Y. Dai, J. Rashba-Step, and A. I. Cederbaum: Stable expression of human cytochrome P4502E1 in HepG2 cells: characterization of catalytic activities and production of reactive oxygen intermediates. Biochemistry 32, 6928-6937 (1993).
6. S. S. T. Lee, J. T. M. Buters, T. Pineau, P. Fernandez-Salguero, and F. J. Gonzalez: Role of CYP2E1 in the hepatotoxicity of acetaminophen. J. Biol. Chem. 271, 12063-12067 (1996).
7. C. S. Lieber: Biochemical and molecular basis of alcohol-induced injury to liver and other tissues. N. Engl. J. Med. 319, 1639-1650 (1988).
8. C. S. Lieber: Hepatic, metabolic and toxic effects of ethanol. Update Alcohol. Clin. Exp. Res. 15, 573-592 (1991).
9. D. O'Shea, S. N. Davis, R. B. Kim, and G. R. Wilkinson: Effect of fasting and obesity in humans on the 6-hydroxylation of chlorzoxazone: a putative probe of CYP2E1 activity. Clin. Pharmacol. Ther. 56, 359-367 (1994).
10. S. A. Wrighton, P. E. Thomas, D. T. Molowa, M. Hanui, J. E. Shively, S. L. Maines, P. B. Watkins, G. Mendez-Picon, W. Levin, and P. S. Guzelian: Characterization of ethanol-inducible human liver N-nitrosodimethylamine demethylase. Biochemistry 25, 6731-6735 (1986).
11. M. Tsutsumi, J. M. Lasker, M. Shimizu, A. S. Rosman, and C. S. Lieber: The intralobular distribution of ethanol-inducible P450IIE1 in rat and human liver. Hepatology 10, 437-446 (1989).
12. X. Ding, D. R. Koop, B. L. Crump, and M. J. Coon: Immunochemical identification of cytochrome P4503a (P450ALC) in rabbit nasal and kidney microsomes and evidence for differential induction by alcohol. Mol. Pharmacol. 30, 370-378 (1986).
13. X. Ding and M. J. Coon: Induction of cytochrome P450 isozyme 3a (P450IIE1) in rabbit olfactory mucosa by ethanol and acetone. Drug Metab. Dispos. 18, 742-745 (1990).
14. G. G. Schnier, C. L. Laethem, and D. R. Koop: Identification and induction of cytochrome P450, P450IIE1 and P450IA1, in rabbit bone marrow. J. Pharmacol. Exp. Ther. 251, 790-796 (1989).
15. B. J. Song, R. L. Veech, and P. Saenger: Cytochrome P450IIE1 is elevated in lymphocytes from poorly controlled insulin-dependent diabetics. J. Clin. Endocrinol. Metab. 71, 1036-1040 (1990).
16. J. L. Raucy, G. Curley, and S. P. Carpenter: The use of lymphocytes for assessing ethanol-mediated alterations in the expression of hepatic CYP2E1. Alcohol. Clin. Exp. Res. 19, 1369-1375 (1995).
17. Y. Soh, H. M. Rhee, D. H. Sohn, and B. J. Song: Immunological detection of CYP2E1 in fresh rat lymphocytes and its pretranslational induction by fasting. Biochem. Biophys. Res. Commun. 227, 541-546 (1996).
18. 1 450 gene sequence and correlation of mRNA with genetic differences in benzo(a)pyrene metabolism. Nucleic Acids Res. 13, 4503-4520 (1985).
19. S. K. Janardan, K. S. Lown, P. Schmiedlin-Ren, K. E. Thummel, and P. B. Watkins: Selective expression of CYP3A5 and not CYP3A4 in human blood. Pharmacogenetics 6, 379-385 (1996).
20. J. A. Ewing: Detecting alcoholism: the CAGE questionnaire. J. Am. Med. Assoc. 252, 1905-1907 (1984).
21. D. G. Mayfield, G. McLeod, and P. Hall: The CAGE questionnaire: validation of a new alcoholism screening instrument. Am. J. Psychiatry 131, 1121-1123 (1974).
22. B. F. Grant and D. S. Hasin: "Alcohol Use Disorders and Associated Disabilities Interview Schedule (AUDADIS)." National Institute on Alcoholism and Alcohol Abuse, Bethesda, MD, 1990.
23. A. Boyum: Separation of leukocytes from blood and bone marrow. Scand. J. Clin. Lab. Invest. 21, 77 (1968).
24. J. L. Raucy and J. M. Lasker: Isolation of P450 enzymes from human liver. Methods Enzymol. 206, 577-587 (1991).
25. S. P. Carpenter, J. M. Lasker, and J. L. Raucy: Expression, induction, and catalytic activity of the ethanol-inducible cytochrome P450 (CYP2E1) in human fetal liver and hepatocytes. Mol. Pharmacol. 49, 260-268 (1996).
26. A. Bensadoun and D. Weinstein: Assay of proteins in the presence of interfering materials. Anal. Biochem. 162, 156-159 (1987).
27. J. L. Raucy, J. M. Lasker, C. S. Lieber, and M. Black: Acetaminophen activation by human liver cytochromes P450IIE1 and P450IA2. Arch. Biochem. Biophys. 271, 270-283 (1989).
28. F. Sanger, S. Nicklen, and A. R. Colson: DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74, 5463-5467 (1987).
29. B.-J. Song, H. V. Gelboin, S. S. Park, C. S. Yang, and F. J. Gonzalez: Complementary DNA and protein sequences of ethanol-inducible rat and human cytochrome P450s. J. Biol. Chem. 261, 16689-16697 (1986).
30. D. Lucas and F. Berthou: High-performance liquid chromatographic determination of chlorzoxazone and 6-hydroxychlorzoxazone in serum: a tool for indirect evaluation of cytochrome P4502E1 activity in humans. J. Chromatogr. 622, 79-86 (1993).
31. A. W. Dreisbach, N. Ferencz, N. E. Hopkins, M. G. Fuentes, A. B. Rege, W. J. George, and J. J. L. Lertora: Urinary excretion of 6-hydroxychlorzoxazone as an index of CYP2E1 activity. Clin. Pharmacol. Ther. 58, 498-505 (1995).
32. K. Bachmann and J. G. Sarver: Chlorzoxazone as a single sample probe of hepatic CYP2E1 activity in humans. Pharmacology 52, 169-177 (1996).
33. E. D. Kharasch, K. E. Thummel, J. Mhyre, and J. H. Lillibridge: Single-dose disulfiram inhibition of chlorzoxazone metabolism: a clinical probe for P450 2E1. Clin. Pharmacol. Ther. 53, 643-650 (1993).
34. S. Ono, T. Hatanaka, H. Hotta, M. Tsutsui, T. Satoh, and F. J. Gonzalez: Chlorzoxazone is metabolized by human CYP1A2 as well as by human CYP2E1. Pharmacogenetics 5, 141-148 (1995).
35. J. C. Gorski, D. R. Jones, S. A. Wrighton, and S. D. Hall: Contribution of human CYP3A subfamily members to the 6-hydroxylation of chlorzoxazone. Xenobiotica 27, 243-256 (1997).
36. C. Girre, D. Lucas, E. Hispard, C. Ménez, S. Dally, and J.-F. Ménez: Assessment of cytochrome P4502E1 induction in alcoholic patients by chlorzoxazone pharmacokinetics. Biochem. Pharmacol. 47, 1503-1508 (1994).
37. D. Lucas, C. Menéz, C. Girre, P. Bodenéz, E. Hispard, and J.-F. Menéz: Decrease in cytochrome P4502E1 as assessed by the rate of chlorzoxazone hydroxylation in alcoholics during the withdrawal phase. Alcohol. Clin. Exp. Res. 19, 362-366 (1995).
38. D. R. Koop and D. J. Tierney: Multiple mechanisms in the regulation of ethanol-inducible cytochrome P450IIE1. Bioessays 12, 429-435 (1990).
39. V. E. Kostrubsky, S. C. Strom, S. G. Wood, S. A. Wrighton, P. R. Sinclair, and J. F. Sinclair: Ethanol and isopentanol increase CYP3A and CYP2E in primary cultures of human hepatocytes. Arch. Biochem. Biophys. 322, 516-520 (1995).
40. M. X. Yang and A. I. Cederbaum: Characterization of cytochrome P4502E1 turnover in transfected HepG2 cells expressing human CYP2E1. Arch. Biochem. Biophys. 341, 25-33 (1997).
41. A. J. Treves, D. Yagoda, A. Haimovitz, N. Ramu, D. Rachmilewitz, and Z. Fuks: The isolation and purification of human peripheral blood monocytes in cell suspension. J. Immunol. Methods 39, 71-80 (1980).