Critical residues of Chlamydomonas reinhardtii ferredoxin for interaction with nitrite reductase and glutamate synthase revealed by site-directed mutagenesis

European Journal of Biochemistry

Volumn 250, Issue 2, 1997, Pages 364-368

  García Sánchez, M Isabel ^a   Gotor, Cecilia ^a   Jacquot, Jean Pierre ^b   Stein, Mariana ^b   Suzuki, Akira ^c   Vega, José M ^a,d  

^a UNIVERSITY OF SEVILLE   (Spain)

^b INSTITUTE OF PLANT SCIENCES PARIS SACLAY IPS2   (France)

^c CEMAGREF   (France)

^d Universidad de Sevilla CSIC   (Spain)

Author keywords

Binding domain; Ferredoxin; Glutamate synthase; Nitrite reductase; Site directed mutagenesis

Indexed keywords

FERREDOXIN; GLUTAMATE SYNTHASE; NITRITE REDUCTASE;

ARTICLE; BINDING SITE; CHLAMYDOMONAS REINHARDTII; NONHUMAN; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS;

CHLAMYDOMONAS REINHARDTII;

EID: 0031437209     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1432-1033.1997.0364a.x     Document Type: Article

References (17)

1. García-Sánchez, M. L., Vigara, A. J., Gotor, C. & Vega, J. M. (1995) A new method to identify ferredoxin-interacting proteins, in Photosynthesis: from light to biosphere (Mathis, P., ed.) vol. 2, pp. 685-688, Kluwer Academic Publishers, Dordrecht.
2. Hurley, J. K., Fillat, M. Gómez-Moreno, C. & Tollin, G. (1995) Structure-function relationships in the ferredoxin/ferredoxin: NADP reductase system from Anabaena, Biochimie (Paris) 77, 539-548.
3. Jacquot, J.-P., Stein, M., Suzuki, A., Liottet, S., Sandoz, G. & Miginiac-Maslow, M. (1997) Residue Glu-91 of Chlamydomonas reinhardtii ferredoxin is essential for electron transfer to ferredoxin-thioredoxin reductase, FEBS Lett. 400, 293-296.
4. Knaff, D. B. & Hirasawa, M. (1991) Ferredoxin-dependent chloroplast enzymes, Biochim. Biophys. Acta 1056, 93-125.
5. Márquez, A. J., Gotor, C., Romero, L. C., Galván, F. & Vega, J. M. (1986) Ferredoxin-glutamate synthase from Chlamydomonas reinhardtii. Prosthetic groups and preliminary studies of mechanism, Int. J. Biochem. 18, 531-535.
6. Navarro, F., Chávez, S., Candau, P. & Florencio, F. J. (1995) Existence of two ferredoxin-glutamate synthases in the cyanobacterium Synechocystis sp. PCC 6803. Isolation and insertional inactivation of gltB and gltS genes, Plant Mol. Biol. 27, 753-767.
7. Piubelli, L., Aliverti, A., Bellintani, F. & Zanetti, G. (1996) Mutations of Glu92 in ferredoxin I from spinach leaves produce proteins fully functional in electron transfer but less efficient in supporting NADP photoreduction, Eur. J. Biochem. 236, 465-469.
8. Privalle, L. S., Privalle, C. T., Leonardy, N. J. & Kamin, H. (1985) Interactions between spinach ferredoxin-nitrite reductase and its substrates, J. Biol. Chem. 260, 14344-14350.
9. Rogers, W. J., Hodges, M., Decottignies, P., Schmitter, J.-M. Gadal, P. & Jacquot, J.-P. (1992) Isolation of a cDNA fragment coding for Chlamydomonas reinhardtii ferredoxin and expression of the recombinant protein in Escherichia coli, FEBS Lett. 310, 240-245.
10. Romero, L. C., Galván, F. & Vega, J. M. (1987) Purification and properties of the siroheme-containing ferredoxin-nitrite reductase from Chlamydomonas reinhardtii, Biochim. Biophys. Acta 914, 55-63.
11. Romero, L. C., Gotor, C., Márquez, A. J., Forde, B. G. & Vega, J. M. (1988) Antigenic similarities between ferredoxin-dependent nitrite reductase and glutamate synthase from Chlamydomonas reinhardtii, Biochim. Biophys. Acta 957, 152-157.
12. Sambrook, J., Fritsch, E. F. & Maniatis, T. (1989) Molecular cloning: a laboratory manual, 2nd edn, Cold Spring Harbor Laboratory, Cold Spring Harbor NY.
13. Schmitz, S., Navarro, F., Kutzki, C. K., Florencio, F. J. & Böhme, H. (1996) Glutamate 94 of [2Fe-2S]-ferredoxins is important for efficient electron transfer in the 1 : 1 complex formed with ferredoxin-glutamate synthase (GltS) from Synechocystis sp. PCC 6803, Biochim. Biophys. Acta 1277, 135-140.
14. Vega, J. M. & Kamin, H. (1977) Spinach nitrite reductase, purification and properties of a siroheme-containing iron-sulfur enzyme, J. Biol. Chem. 252, 896-909.
15. Vieira, B. J., Colvert, K. K. & Davis, D. J. (1986) Chemical modification and cross-linking as probes of regions on ferredoxin involved in its interaction with ferredoxin: NADP reductase, Biochim. Biophys. Acta 851, 109-122.
16. Vigara, A. J., Gómez-Moreno, C. & Vega, J. M. (1995) The role of flavodoxin in the reaction catalysed by the glutamate synthase from Monoraphidium braunii, Bioelectrochem. Bioenerg. 38, 21-24.
17. Vigara, A. J., García-Sánchez, M. I., Gotor, C. & Vega, J. M. (1996) Interaction between glutamate synthase and ferredoxin from Monoraphidium braunii. Chemical modifications and cross-linking tudies, Plant Physiol. Biochem. 34, 707-711.