Detection of antisperm antibodies in seminal plasma by flow cytometry: Comparison with the indirect immunobead binding test

Fertility and Sterility

Volumn 68, Issue 6, 1997, Pages 1114-1119

  Nicholson, Simon C ^a,b   Robinson, Julian N ^a   Sargent, Ian L ^a   Barlow, David H ^a  

^a JOHN RADCLIFFE HOSPITAL   (United Kingdom)

^b DEPARTMENT OF OBSTETRICS AND GYNAECOLOGY   (United Kingdom)

Author keywords

Antisperm antibody; Flow cytometry; Immunobead binding test; Seminal plasma

Indexed keywords

SPERM ANTIBODY;

ANTIBODY DETECTION; ARTICLE; DIAGNOSTIC ACCURACY; DIAGNOSTIC VALUE; FLOW CYTOMETRY; HUMAN; MALE; MALE INFERTILITY; PRIORITY JOURNAL; SEMEN ANALYSIS; SEMINAL PLASMA;

AUTOANTIBODIES; BINDING SITES, ANTIBODY; FLOW CYTOMETRY; HUMANS; IMMUNOASSAY; MALE; PROSPECTIVE STUDIES; SEMEN; SPERMATOZOA;

EID: 0031436290     PISSN: 00150282     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0015-0282(97)00374-9     Document Type: Article

References (22)

1. 1. Hendry WF. The significance of antisperm antibodies: measurement and management. Clin Endocrinol 1992;36:219-21.
2. 2. Bronson R, Cooper G, Hjort T, Ing R, Jones WR, Wang SX, et al. Anti-sperm antibodies, detected by agglutination, immobilization, microcytotoxicity and immunobead-binding assays. J Reprod Immunol 1985;8:279-99.
3. 3. Jager S, Kremer J, Van Slochteren-Draaisma T. A simple method for screening for antisperm antibodies in the human male. Int J Fertil 1978;23:12-8.
4. 4. Meinertz H, Bronson R. Detection of antisperm antibodies on the surface of motile spermatozoa. Comparison of the immunobead binding technique (IBT) and the mixed antiglobulin reaction (MAR). Am J Reprod Immunol Microbiol 1988;18: 120-3.
5. 5. Haas GG, Cunningham ME. Identification of antibody-laden sperm by cytofluorometry. Fertil Steril 1984;42:606-13.
6. 6. Rasanen ML, Hovatta OL, Penttila IM, Agrawal YP. Detection and quantitation of sperm-bound antibodies by flow cytometry of human semen. J Androl 1992;13:55-64.
7. 7. Nikolaeva MA, Kulakov VI, Ter AG, Terekhina LN, Pshenichnikova TJ, Sukhikh GT. Detection of antisperm antibodies on the surface of living spermatozoa using flow cytometry: preliminary study. Fertil Steril 1993;59:639-44.
8. 8. Ke RW, Dockter ME, Majumdar G, Buster JE, Carson SA. Flow cytometry provides rapid and highly accurate detection of antisperm antibodies. Fertil Steril 1995;63:902-6.
9. 9. Haas GG, D'Cruz O, DeBault L. Comparison of the indirect immunobead, radiolabelled and immunofluorescence assays for immunoglobulin G serum antibodies to human sperm. Fertil Steril 1991;55:377-88.
10. 10. Rasanen M, Agrawal YP, Saarikoski S. Seminal fluid antisperm antibodies measured by direct flow cytometry do not correlate with those measured by indirect flow cytometry, the indirect immunobead test, and the indirect mixed antiglobulin reaction. Fertil Steril 1996;65:170-5.
11. 11. Mortimer D. Semen analysis and sperm washing techniques. In: Gagnon C, editor. Controls of sperm motility: biological and clinical aspects. Boca Raton (FL): CRC Press, 1990:263-84.
12. 12. Barratt CLR, Dunphy BC, McLeod I, Cooke ID. The poor prognostic value of low to moderate levels of sperm surface-bound antibodies. Hum Reprod 1992;7:95-8.
13. 13. World Health Organization. Laboratory manual for the examination of human semen and semen-cervical mucus interaction. 3rd ed. New York: Cambridge University Press, 1993: 66-8.
14. 14. Fleis JL. Statistical methods for rates and proportions. Toronto: John Wiley, 1981.
15. 15. Landis RJ, Koch GG. The measurement of observer agreement for categorical data. Biometrics 1977;33:159-74.
16. 16. Hendry WF, Hughes L, Scammell G, Pryor J, Hargreave TB. Comparison of prednisolone and placebo in subfertile men with antibodies to spermatozoa. Lancet 1990;335:85-8.
17. 17. Yovich JL, Matson PL. The treatment of infertility by the high intrauterine insemination of husband's washed spermatozoa. Hum Reprod 1988;3:939-43.
18. 18. Van DMJ, Kruger TF, Windt ML, Hulme VA, Menkveld R. Treatment of male sperm autoimmunity by using the gamete intrafallopian transfer procedure with washed spermatozoa. Fertil Steril 1990;53:682-7.
19. 19. Naaktgeboren N, Devroey P, Van SA. Successful in vitro fertilization with sperm cells from a man with immune fertility. Ann. N Y Acad Sci 1985;442.
20. 20. Bronson R, Cooper G, Rosenfeld D. Sperm antibodies: their role in infertility. Fertil Steril 1984;42:171-83.
21. 21. Bronson RA, Cooper GW, Rosenfeld DL. Seminal fluid antisperm antibodies do not reflect those present on the sperm surface. Fertil Steril 1987;48:505-6.
22. 22. Hellstrom W, Overstreet JW, Moore SM, Samuels SJ, Chang RJ, Lewis EL. Antisperm antibodies bind with different patterns to sperm of different men. J Urol 1987;138:895-8.