Production and purification of an extracellular β-galactosidase from the Dutch elm disease fungus Ophiostoma novo-ulmi

Canadian Journal of Microbiology

Volumn 43, Issue 11, 1997, Pages 1011-1016

  Binz, Thomas ^a,b   Gremaud, Colette ^a   Canevascini, Giorgio ^a  

^a Inst de Biol Veg   (Switzerland)

^b UNIVERSITY OF TORONTO   (Canada)

Author keywords

Dutch elm disease; Ophiostoma novo ulmi; Ophiostoma ulmi; galactosidase

Indexed keywords

BETA GALACTOSIDASE;

ARTICLE; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME PURIFICATION; ENZYME SYNTHESIS; ISOELECTRIC POINT; NONHUMAN; OPHIOSTOMALES; PRIORITY JOURNAL;

FUNGI; OPHIOSTOMA NOVO; OPHIOSTOMA NOVO-ULMI; OPHIOSTOMA ULMI; OPHIOSTOMATALES; ULMUS;

EID: 0031436024     PISSN: 00084166     EISSN: None     Source Type: Journal    
DOI: 10.1139/m97-146     Document Type: Article

References (30)

1. Ali, Z.M., Armugam, S., and Lazan, H. 1995. β-Galactosidase and its significance in ripening mango fruit. Phytochemistry, 38: 1109-1114.
2. Binz, T., and Canevascini, G. 1996a. Differential production of extracellular laccase in the Dutch elm disease pathogens Ophiostoma ulmi and O. novo-ulmi. Mycol. Res. 100: 1060-1064.
3. Binz, T., and Canevascini, G. 1996b. Xylanases from the Dutch elm disease pathogens Ophiostoma ulmi and O. novo-ulmi. Physiol. Mol. Plant Pathol. 49: 159-175.
4. Bradford, M.M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248-254.
5. Brunton, A.H., and Gadd, G.M. 1991. Evidence for an inositol lipid signal pathway in the yeast-mycelium transition of Ophiostoma ulmi, the Dutch elm disease fungus. Mycol. Res. 95: 484-491.
6. Buckeridge, M.S., and Reid, J.S.G. 1994. Purification and properties of a novel β-galactosidase or exo-(1,4)-β-D-galactanase from the cotyledons of germinated Lupinus angustifolius L. seeds. Planta, 192: 502-511.
7. Cooper, R.M. 1983. The mechanisms and significance of enzymic degradation of host cell walls by parasites. In Biochemical plant pathology. Edited by C.A. Callow. John Wiley and Sons Ltd., New York. pp. 101-135.
8. Cooper, R.M., and Wood, R.K.S. 1975. Regulation of synthesis of cell wall degrading enzymes by Verticillium albo-atrum and Fusarium oxysporum f.sp. lycopersici. Physiol. Plant Pathol. 5: 135-156.
9. Cooper, R.M., Longman, D., Campbell, A., Henry, M., and Lees, P.E. 1988. Enzymic adaptation of cereal pathogens to the monocotyledonous primary wall. Physiol. Mol. Plant Pathol. 32: 33-47.
10. Dickson, R.C., Dickson, L.R., and Markin, J.S. 1979. Purification and properties of an inducible β-galactosidase from the yeast Kluyveromyces lactis. J Bacteriol. 37: 51-61.
11. Dubois, M., Gilles, K.A., Hamilton, J.K., Rebers, P.A., and Smith, F. 1956. Colorimetric method for the determination of sugars and related substances. Anal. Chem. 28: 350-356.
12. Elgersma, D.M. 1976. Production of pectic and cellulolytic enzymes by aggressive and non-aggressive strains of Ophiostoma ulmi. Neth. J. Plant Pathol. 82: 161-172.
13. Golden, K.D., Melford, A.J., and Kean, E.A. 1993. β-Galactosidase from Coffea arabica and its role in fruit ripening. Phytochemistry, 34: 355-360.
14. Goncalves, J.A., and Castillo, F.J. 1982. Partial purification and characterization of β-galactosidase from Kluyveromyces marxianus. J. Diary Sci. 65: 2088-2094.
15. Goodman, R.N., Kiraly, Z., and Wood, K.R. 1986. Cell wall composition and metabolism. In The biochemistry and physiology of plant disease. Edited by R.N. Goodman, Z. Kiraly, and K.R. Wood. University of Missouri Press, Columbia, Mo.
16. Gough, C.L., Dow, J.M., Barber, C.E., and Daniels, M.J. 1988. Cloning of two endoglucanase genes of Xanthomonas campestris pv campestris: analysis of the role of the major endoglucanase in pathogenesis. Mol. Plant-Microbe Interact. 1: 275-281.
17. Guy, E.J., and Bingham, E.W. 1978. Properties of β-galactosidase of Saccharomyces lactis in milk and milk products. J. Diary. Sci. 61: 147-151.
18. Jones, T.M., Anderson, A.J., and Albersheim, P. 1972. Host-pathogen interactions IV. Studies on the polysaccharide-degrading enzymes secreted by Fusarium oxysporum f.sp. lycopersici. Physiol. Plant Pathol. 2: 153-166.
19. Laemmli, U.K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London), 227: 680-685.
20. Miller, H.J., and Elgersma, D.M. 1976. The growth of aggressive and non-aggressive strains of Ophiostoma ulmi in susceptible and resistant elms, a scanning microscopical study. Neth. J. Plant Pathol. 82: 51-65.
21. Nakao, M., Harada, M., Kodama, Y., Nakayama, T., Shibano, Y., and Amachi, T. 1994. Purification and characterization of a thermostable β-galactosidase with high transgalactosylation activity from Saccharopolyspora rectivirgula. Appl. Microbiol. Biotechnol. 40: 657-663.
22. Nelson, N. 1944. A photometric adaptation of the Somogyi method for the determination of glucose. J. Biol. Chem. 153: 375-380.
23. Ohtsuka, K., Tanoh, A., Ozawa, O., Kanematsu, T., Uchida, T., and Shinke, R. 1990. Purification and properties of a β-galactosidase with high galactosyl transfer activity from Cryptococcus laurentii OKN-4. J. Ferment. Bioeng. 70: 301-307.
24. Park, Y.K., DeSanti, M.S.S., and Pastore, G.M. 1979. Production and characterization of β-galactosidase from Aspergillus oryzae. J. Food Sci. 44: 100-103.
25. Pegg, G.F. 1985. Presidential address. Life in a black hole - the microenvironment of the vascular pathogen. Trans. Br. Mycol. Soc. 85: 1-20.
26. Riou, C., Freyssinet, G., and Fevre, M. 1991. Production of cell wall-degrading enzymes by the phytopathogenic fungus Sclerotinia sclerotiorum. Appl. Environ. Microbiol. 57: 1478-1484.
27. Schell, M.A., Roberts, D.P., and Denny, T.P. 1988. Analysis of the Pseudomonas solanacearum polygalacturonase encoded by pg1A and its involvement in phytopathogenicity. J. Bacteriol. 170: 4501-4508.
28. Svaldi, R., and Elgersma, D.M. 1982. Further studies of the activity of cell wall degrading enzymes of aggressive and non-aggressive isolates of Ophiostoma ulmi. Eur. J. For. Pathol. 12: 29-36.
29. Valero, P., and Labrador, E. 1993. Inhibition of cell wall autolysis and auxin-induced elongation of Cicer arietinum epicotyls by β-galactosidase antibodies. Physiol. Plant. 89: 199-203.
30. Walton, J.D. 1994. Deconstructing the cell wall. Plant Physiol. 104: 1113-1118.