Use of a membrane-localized green fluorescent protein allows simultaneous identification of transfected cells and cell cycle analysis by flow cytometry

Cytometry

Volumn 29, Issue 4, 1997, Pages 286-291

  Kalejta, Robert F ^a   Shenk, Thomas ^a   Beavis, Andrew J ^a  

^a PRINCETON UNIVERSITY   (United States)

Author keywords

Cell cycle; Flow cytometry; Fusion protein; Green fluorescent protein; Propidium iodide staining; transient transfection

Indexed keywords

ALCOHOL; DNA; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; PARAFORMALDEHYDE; PROPIDIUM IODIDE; PROTEIN P53; SPECTRIN;

ARTICLE; CELL CYCLE; CELL MEMBRANE PERMEABILITY; FIBROBLAST; FLOW CYTOMETRY; FLUORESCENCE; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; OSTEOSARCOMA CELL; PREPUCE; PRIORITY JOURNAL; PROTEIN LOCALIZATION;

BIOLOGICAL MARKERS; BONE NEOPLASMS; CELL CYCLE; CELL MEMBRANE; DNA, NEOPLASM; ELECTROPORATION; FIBROBLASTS; FLOW CYTOMETRY; GREEN FLUORESCENT PROTEINS; HUMANS; INDICATORS AND REAGENTS; LUMINESCENT PROTEINS; N-ACETYLLACTOSAMINE SYNTHASE; OSTEOSARCOMA; PROPIDIUM; TRANSFECTION;

EID: 0031435461     PISSN: 01964763     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0320(19971201)29:4<286::AID-CYTO4>3.0.CO;2-8     Document Type: Article

References (20)

1. Chalfie M, Tu Y, Euskirchen G, Ward WW, Prasher DC: Green fluorescent protein as a marker for gene expression. Science 263:802-805, 1994.
2. Cole NB, Smith CL, Sciaky N, Teraski M, Edidin M, Lippincott-Schwartz J: Diffusional mobility of Golgi proteins in membranes of living cells. Science 273:797-801, 1996.
3. Cormack BP, Valdivia RH, Falkow S: FACS-optimized mutants of the green fluorescent protein (GFP). Gene 173:33-38, 1996.
4. El-Diery W, Tokino T, Velculescu VE, Levy DB, Parsons R, Trent JM, Lin D, Mercer WE, Kanzler KW, Vogelstein B: WAF1, a potential mediator of p53 tumor suppression. Cell 75:817-825, 1993.
5. Fiering SN, Roederer M, Nolan GP, Micklem DR, Parks DR, Herzenberg LA: Improved FACS-Gal: Flow cytometric analysis and sorting of viable eukaryotic cells expressing reporter gene constructs. Cytometry 12:291-301, 1991.
6. Gong J, Traganos F, Darzynkiewicz Z: A selective procedure for DNA extraction from apoptotic cells applicable for gel electrophoresis and flow cytometry. Anal Biochem 218:314-319, 1994.
7. Gossen M, Bujard H: Tight control of gene expression in mammalian cells by tetracycline-responsive promoters. Proc Natl Acad Sci USA 89:5547-5551, 1992.
8. He J, Choe S, Walker R, Di Marzio P, Morgan DO, Landau NR: Human immunodeficiency virus type 1 viral protein R (Vpr) arrests cells in the G2 phase of the cell cycle by inhibiting p34cdc2 activity. J Virol 69:6705-6711, 1995.
9. Levine AJ: p53, the cellular gatekeeper for growth and division. Cell 88:323-331, 1997.
10. Lin J, Chen J, Elenbaas B, Levine A: Several hydrophobic amino acids in the p53 amino-terminal domain are required for transcriptional activation, binding to mdm-2 and the adenovirus 5 E1B 55-kD protein. Genes Dev 8:1235-1246, 1994.
11. Naumovski L, Cleary ML: The p53-binding protein 53BP2 also interacts with Bcl 2 and impedes cell cycle progression at G2/M. Mol Cell Biol 16:3884-3892, 1996.
12. Prasher DC, Eckenrode VK, Ward WW, Pendergast FG, Cormier MJ: Primary structure of the Aeqorea victoria green-fluorescent protein. Gene 111:229-233, 1992.
13. 1/S phase transition by expression of cyclins D1 and E with an inducible system. Mol Cell Biol 14:1669-1679, 1994.
14. Kip1. Mol Cell Biol 15:4347-4352, 1995.
15. Shapiro HM: Practical Flow Cytometry, Ed. 3. John Wiley & Sons, New York, 1995, pp 251-253.
16. van den Heuvel S, Harlow E: Distinct roles for cyclin-dependent kinases in cell cycle control. Science 262:2050-2054, 1993.
17. Wang D-S, Miller R, Shaw R, Shaw G: The pleckstrin homology domain of human βIΣII spectrin is targeted to the plasma membrane in vivo. Biochem Biophys Res Commun 225:420-426, 1996.
18. Wigler M, Sweet R, Sim GK, Wold B, Pellicer A, Lacy E, Maniatis T, Silverstein S, Axel R: Transformation of mammalian cells with genes from prokaryotes and eukaryotes. Cell 16:777-785, 1979.
19. Xiao Z-X, Chen J, Levine AJ, Modjtahedi N, Xing J, Sellers WR, Livingston DM: Interaction between the retinoblastoma protein and the oncoprotein MDM2. Nature 375:694-698, 1995.
20. Zhu L, van den Heuvel S, Helin K, Fattaey A, Ewen M, Livingston D, Dyson N, Harlow E: Inhibition of cell proliferation by p107, a relative of the retinoblastoma protein. Genes Dev 7:1111-1125, 1993.