Construction of a gene encoding the insect bactericidal protein attacin. Studies on its expression in Escherichia coli

Biological Research

Volumn 30, Issue 4, 1997, Pages 149-160

  Santibañez, Eudocia ^a   Gomez, Isabel ^a   Martinez, Maria Teresa ^a   Bruce, Elsa ^a   Venegas, Alejandro ^a  

^a PONTIFICIA UNIVERSIDAD CATÓLICA DE CHILE   (Chile)

Author keywords

Attacin cDNA; Bacterial expression; Bactericidal peptide; pET vector

Indexed keywords

COMPLEMENTARY DNA; INSECT PROTEIN; METHIONINE; NALIDIXIC ACID; RNA POLYMERASE;

ANIMAL CELL; ARTICLE; BACTERIAL GENE; BACTERIAL GROWTH; BACTERICIDAL ACTIVITY; CYTOPLASM; ESCHERICHIA COLI; EXPRESSION VECTOR; FLUOROGRAPHY; GEL ELECTROPHORESIS; GENE EXPRESSION REGULATION; GENE SEQUENCE; IMMUNOBLOTTING; ISOTOPE LABELING; LYSIS; MOLECULAR CLONING; MOTH; NONHUMAN; PLASMID; POLYMERASE CHAIN REACTION; PROTEIN EXPRESSION; STRUCTURAL GENE;

ANTI-BACTERIAL AGENTS; BASE SEQUENCE; CLONING, MOLECULAR; DNA, BACTERIAL; DNA, COMPLEMENTARY; ESCHERICHIA COLI; GENE AMPLIFICATION; GENE EXPRESSION REGULATION, BACTERIAL; INSECT HORMONES; MOLECULAR SEQUENCE DATA; NUCLEOTIDES;

ANIMALIA; BACTERIA (MICROORGANISMS); BOMBYX MORI; CECROPIA; ESCHERICHIA COLI; HYALOPHORA CECROPIA; INSECTA; LEPIDOPTERA;

EID: 0031435426     PISSN: 07169760     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (35)

1. ANDO K, OKADA M, NATORI S (1987) Purification of sarcotoxin II, antibacterial proteins of Sarcophaga peregrina (flesh fly) larvae. Biochemistry 26: 226-230
2. ANDO K, NATORI S (1988) Inhibitory effect of sarcotoxin IIA, an antibacterial protein of Sarcophaga peregrina, on growth of Escherichia coli. J Biochem. Tokyo 103: 735-739
3. BIRNBOIM HC, DOLY J (1979) A rapid alkaline extraction procedure for screening recombinant plasmid DNA . Nucleic Acids Res 7: 1513-1523
4. BOMAN HG, FAYE I, van HOFSTEN P, KOCKUM K, LEE J, XANTHOPOULOS KG (1985) On the primary structures of lysozyme, cecropins and attacins from Hyalophora cecropia. Dev Comp Immunol 9: 551-558
5. BREITLOW S (1992) Selection of protein stains for SDS gel electrophoresis. Promega Corporation, Promega Notes 35: 15-17
6. CARLSSON A, ENGSTRÖM P, PALVA T, BENNICH H (1991) Attacin, an antibacterial protein from Hyalophora cecropia, inhibits synthesis of outer membrane proteins in Escherichia coli by interfering with omp gene transcription. Infect Immun 59: 3040-3045
7. CHAMBERLAIN JP (1979) Fluorographic detection of radioactivity in polyacrylamide gels with the water-soluble fluor, sodium salicylate. Anal Biochem 98: 132-136
8. CHEN EY, SEEBURG PH (1985) Supercoil sequencing: A fast and simple method for sequencing plasmid DNA. DNA 4: 165-170
9. DICKINSON L, RUSSEL V, DUNN PE (1988) A family of bacteria-regulated, cecropin D-like peptides from Munduca sexta. J Biol Chem 263: 19424-19429
10. DIMARCQ JL, KEPPI E, DUNBAR B, LAMBERT J, REICHHART J-M, HOFFMANN D, RANKINE SM, FOTHERGILL JE, HOFFMANN JA (1988) Insect immunity. Purification and characterization of a family of novel inducible antibacterial proteins from immunized larvae of the dipteran Phormia terranovae and complete amino-acid sequence of the predominant member, diptericin A. Eur J Biochem 171: 17-22
11. ENGSTRÖM P, CARLSSON A, ENGSTRÖM Å, TAO Z-J, BENNICH H (1984) The antibacterial effect of attacins from the silk moth Hyalophora cecropia is directed against the outer membrane of Escherichia coli. EMBO J 3: 3347-3351
12. HANAHAN D (1983) Studies on transformation of Escherichia coli with plasmids. J Mol Biol 166: 557-580
13. HARLOW DE, LANE D (1988) Antibodies: A laboratory manual. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press
14. HAWKES R (1986) The dot immunobinding assay. Meth Enzymol 121: 484-491
15. HULTMARK D, ENGSTRÖM Å, ANDERSSON K, STEINER H, BENNICH H, BOMAN HG (1983) Insect immunity. Attacins, a family of antibacterial proteins from Hyalophora cecropia. EMBO J 2: 571-576
16. HULTMARK D (1993) Immune reactions in Drosophila and other insects: a model for innate immunity. Trends Genet 9:178-183
17. ISHIKAWA M, KUBO T, NATORI S (1992) Purification and characterization of a diptericin homologue from Sarcophaga peregrina (flesh fly). Biochem J 287: 573-578
18. KOCKUM K, FAYE I, van HOFSTEN P, LEE JY, XANTHOPOULOS KG, BOMAN HG (1984) Insect immunity. Isolation and sequence of two cDNA clones corresponding to acidic and basic attacins from Hyalophora cecropia. EMBO J 3:2071-2075
19. LAEMMLI UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685
20. LAMBERT J, KEPPI E, DIMARCQ JL, WICKER C, REICHHART JM, DUNBAR B, LEPAGE P, VAN DORSSELAER A, HOFFMANN J, FOTHERGILL J, HOFFMANN D (1989) Insect immunity: isolation from immune blood of the dipteran Phormia terranovae of two insect antibacterial peptides with sequence homology to rabbit lung macrophage bactericidal peptides. Proc Natl Acad Sci USA 86: 262-266
21. LEVIN DE, YAMASAKI E, AMES BN (1982) A new Salmonella tester strain, TA97, for the detection of frameshift mutagens. A run of cytosines as a mutational hot-spot. Mutat Res 94: 315-330
22. MEIZE L (1991) Direct purification of PCR product from amplification reaction. Promega Corporation, Promega Notes 34: 12-14.
23. L promoter: Strategies for overproducing transcription termination factor p. Proc Natl Acad Sci USA 82: 88-92
24. PTASHNE M (1992) Control circuits-setting the switch. In: A genetic switch. Phage lambda and higher organisms. 2nd ed. Cambridge, MA: Blackwell Scientific Publications & Cell Press. pp 49-67
25. SAIKI RK (1989) The design and optimization of the PCR. In: ERLICH HA (ed) PCR Technology. Principles and applications for DNA amplification. New York: Slockton Press. pp 7-16
26. SAMBROOK J, FRITSCH EF, MANIATIS T (1989) Molecular cloning: A laboratory manual. 2nd ed. Cold Spring Harbor. New York: Cold Spring Harbor Laboratory Press
27. SANGER F, NICKLEN S, COULSON AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74: 5463-5467
28. STUDIER FW, ROSENBERG AH, DUNN JJ, DUBENDORFF JW (1990) Use of T7 RNA polymerase to direct expression of cloned genes. Meth Enzymol 185: 60-89
29. SUN S-C, LINDSTRÖM I, LEE J-Y, FAYE I (1991) Structure and expression of the attacin genes in Hyalophora cecropia. Eur J Biochem 196: 247-254
30. TABOR S (1990) Expression using the T7 RNA polymerase/promoter system. In: AUSUBEL FM, BRENT R, KINGSTON RE, MOORE DD, SEIDMAN JG, SMITH JA, STRUHL K (eds) Current Protocols in Molecular Biology, Vol 2, suppl 11. Boston, MA: Wiley & Sons, pp 16.2.1-16.2.11
31. TABOR S, RICHARDSON CC (1985) A bacteriophage T7 polymerase/promoter system for controlled exclusive expression of specific genes. Proc Natl Acad Sci USA 82: 1074-1078
32. TOWBIN H, STAEHELIN T, GORDON J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proc Natl Acad Sci USA 76: 4350-4354
33. VOLLER A, BIDWELL DE, BARTLETT A (1976) Enzyme immunoassay in diagnostic medicine: theory and practice. Bull WHO 53: 55-65
34. WESTON A, BROWN MG, PERKINS HR, SAUNDERS JR, HUMPHREYS GO (1981) Transformation of Escherichia coli with plasmid deoxyribonucleic acid: calcium-induced binding of deoxyribonucleic acid to whole cells and to isolated membrane fractions. J Bacteriol 145: 780-787
35. WICKER C, REICHHART J-M, HOFFMANN D, HULTMARK D, SAMAKOVLIS C, HOFFMANN JA (1990) Insect immunity. Characterization of a Drosophila cDNA encoding a novel member of the diptericin family of immune peptides. J Biol Chem 265: 22493-22498