Characterization of copy number mutants of plasmid pSC101

Journal of General and Applied Microbiology

Volumn 43, Issue 6, 1997, Pages 309-316

  Wadood, Abdul ^a   Dohmoto, Mitsuko ^a   Sugiura, Shigeki ^a   Yamaguchi, Kazuo ^a  

^a KANAZAWA UNIVERSITY   (Japan)

Author keywords

Copy number; DNA replication; Dominance test; Plasmid; pSC101

Indexed keywords

CELL PROTEIN; HELICASE; PLASMID DNA;

AMINO ACID SUBSTITUTION; ARTICLE; BACTERIAL GENETICS; CODON; CONTROLLED STUDY; DNA REPLICATION; ESCHERICHIA COLI; GENE MUTATION; NONHUMAN; PROTEIN DNA BINDING; REPRESSOR GENE;

EID: 0031429978     PISSN: 00221260     EISSN: None     Source Type: Journal    
DOI: 10.2323/jgam.43.309     Document Type: Article

References (33)

1. 3 molecular weight plasmid-encoded protein is required for replication and copy number control in the plasmid pSC101 and its temperature-sensitive derivative pHS1. J. Mol. Biol., 175, 331-347.
2. Casadaban, M. J. and Cohen, S. N. (1980) Analysis of gene control signals by DNA fusion and cloning in Escherichia coli. J. Mol. Biol., 138, 179-207.
3. Chattoraj, D. K., Mason, R. J., and Wickner, S. H. (1988) Mini-P1 plasmid replication: The autoregulation-sequestration paradox. Cell, 52, 551-557.
4. Cohen, S. N. and Chang, A. C. Y. (1977) Revised interpretation of the pSC101 plasmid. J. Bacteriol., 132, 734-737.
5. Fueki, T., Sugiura, S., and Yamaguchi, K. (1996) Open strands adjacent to iterons promote the binding of the replication initiator protein (Rep) of pSC101 to the unit sequence of the iterons in vitro. Biochim. Biophys. Acta, 1305, 181-188.
6. Fueki, T. and Yamaguchi, K. (1991) Exonuclease III promotes in vitro binding of the replication initiator protein of plasmid pSC101 to the repeated sequences in the ori region. Biochim. Biophys. Acta. 1089, 325-330.
7. Hasunuma, K. and Sekiguchi, M. (1977) Replication of plasmid pSC101 in Escherichia coli K12: Requirement for dna A function. Mol. Gen. Genet., 154, 225-230.
8. Ingmer, H. and Cohen, S. N. (1993) Excess intracellular concentration of the pSC101 RepA protein interferes with both plasmid DNA replication and partitioning. J. Bacteriol, 175, 7834-7841.
9. Ingmer, H., Fong, E. L., and Cohen, S. N. (1995) Monomer-dimer equilibrium of the pSC101 RepA protein. J. Mol. Biol., 250, 309-314.
10. Ishige, F., Kobayashi, Y., Sugiura, S., Masamune, Y., and Yamaguchi, K. (1987) Properties of the replication protein in copy number mutants of plasmid pSC101. J. Pharmacobio-Dyn., 10, 39.
11. Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 680-685.
12. Linder, P., Churchward, G., and Caro, L. (1983) Plasmid pSC101 replication mutants generated by insertion of the transposon Tn1000. J. Mol. Biol., 170, 287-303.
13. Under, P., Churchward, G., and Xia, G. X., Yu, Y. Y., and Caro, L. (1985) An essential replication gene, repA, of plasmid pSC101 is autoregulated. J. Mol. Biol., 181, 383-393.
14. Manen, D., Upegui-Gonzalez, L., and Caro, L. (1992) Monomer and dimer of the RepA protein in plasmid pSC101 replication: Domains in RepA. Proc. Natl. Acad. Sci. U.S.A., 89, 8923-8927.
15. Manen, D., Xia, G., and Caro, L. (1994) A locus involved in the regulation of replication in plasmid pSC101. Mol. Microbiol., 11, 875-884.
16. McEachern, M. J., Bott, M. A., Tooker, P. A., and Helinsky, D. R. (1989) Negative control of plasmid R6K replication: Possible role of intermolecular coupling of replication origins. Proc. Natl. Acad. Sci. U.S.A., 86, 7942-7946.
17. Miller, J. (1972) Experiments in Molecular Genetics, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
18. Nordström, K. (1990) Control of plasmid replication - How do DNA iterons set the replication frequency? Cell, 63, 1121-1124.
19. Ohkubo, S. and Yamaguchi, K. (1995) Two enhancer elements for DNA replication of pSC101, par and palindromic binding sequence of the Rep protein. J. Bacteriol., 177, 558-565.
20. Pal, S. K. and Chattoraj, D. K. (1988) P1 plasmid replication: Initiator sequestration is inadequate to explain control by initiator-binding sites. J. Bacteriol., 170, 3554-3560.
21. Saiki, R. K., Gelfand, D. H., Stoffel, S., Scharf, S. J., Higuchi, R., Horn, G. T., Mullis, K. B., and Erlich, H. A. (1988) Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science, 239, 487-491.
22. Sugiura, S., Ohkubo, S., and Yamaguchi, K. (1993) Minimal essential origin of plasmid pSC101 replication: Requirement of the region downstream of iterons. J. Bacteriol., 175, 5993-6001.
23. Sugiura, S., Tanaka, M., Masamune, Y., and Yamaguchi, K. (1990) DNA binding properties of purified replication initiator protein (Rep) encoded by plasmid pSC101. J. Biochem., 107, 369-376.
24. Sugiura, S. and Yamaguchi, K. (1993) A pseudo-symmetric DNA binding motif of purified Rep dimer of plasmid pSC101. J. Biochem., 114, 547-552.
25. Tsurimoto, T. and Matsubara, K. (1981) Purification of bacteriophage λO protein that specifically binds to the origin of replication. Mol. Gen. Genet., 181, 325-331.
26. Vocke, C. and Bastia, D. (1985) The replication initiator protein of plasmid pSC101 is a transcriptional repressor of its own cistron. Proc. Natl. Acad. Sci. U.S.A., 82, 2252-2256.
27. Xia, G. X., Manen, D., Goebel, T., Linder, P., Churchward, G., and Caro, L. (1991) A copy number mutant of plasmid pSC101. MoI. Microbiol., 5, 631-640.
28. Xia, G., Manen, D., Yu, Y., and Caro, L. (1993) In vivo and in vitro studies of a copy number mutation of the RepA replication protein of plasmid pSC101. J. Bacteriol., 175, 4165-4175.
29. Yamaguchi, K. and Masamune, Y. (1985) Autogenous regulation of synthesis of the replication protein in plasmid pSC101. Mol. Gen. Genet., 200, 362-367.
30. Yamaguchi, K. and Tomizawa, J. (1980) Establishment of Escherichia coli cells with an integrated high copy number plasmid. Mol. Gen. Genet., 178, 525-533.
31. Yamaguchi, K. and Yamaguchi, M. (1984a) The replication origin of pSC101: Replication properties of a segment capable of autonomous replication. J. Gen. Appl. Microbiol., 30, 347-358.
32. Yamaguchi, K. and Yamaguchi, M. (1984b) The replication origin of pSC101: The nucleotide sequence and replication functions of the on region. Gene, 29, 211-219.
33. Yanisch-Perron, C., Vieira, J., and Messing, J. (1985) Improved M13 phage cloning vectors and host strains: Nucleotide sequences of the M13mp18 and pUC19 vectors. Gene, 33, 103-119.