메뉴 건너뛰기




Volumn 13, Issue 16, 1997, Pages 1519-1534

Two-dimensional electrophoretic separation of yeast proteins using a non-linear wide range (pH 3-10) immobilized pH gradient in the first dimension; reproducibility and evidence for isoelectric focusing of alkaline (pI>7) proteins

Author keywords

2D PAGE; Protein expression; Protein identification; Proteome analysis; S. cerevisiae

Indexed keywords

ALCOHOL; FUNGAL PROTEIN; GALACTOSE; GLUCOSE;

EID: 0031423822     PISSN: 0749503X     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0061(199712)13:16<1519::AID-YEA211>3.0.CO;2-U     Document Type: Article
Times cited : (73)

References (44)
  • 1
    • 0030895921 scopus 로고    scopus 로고
    • A comparison of selected mRNA and protein abundances in human liver
    • Andersen, L. and Seilhamer, J. (1997). A comparison of selected mRNA and protein abundances in human liver. Electrophoresis 18, 533-537.
    • (1997) Electrophoresis , vol.18 , pp. 533-537
    • Andersen, L.1    Seilhamer, J.2
  • 3
    • 0023309161 scopus 로고
    • Identification of polypeptides of the carbon metabolism machinery on the two-dimensional map of Saccharomyces cerevisiae. Location of 23 additional polypeptides
    • Bataillé, N., Peypouquet, M.-F. and Boucherie, H. (1987). Identification of polypeptides of the carbon metabolism machinery on the two-dimensional map of Saccharomyces cerevisiae. Location of 23 additional polypeptides. Yeast 3, 11-21.
    • (1987) Yeast , vol.3 , pp. 11-21
    • Bataillé, N.1    Peypouquet, M.-F.2    Boucherie, H.3
  • 4
    • 0024117717 scopus 로고
    • Two-dimensional gel analysis of yeast proteins: Application to the study of changes in the levels of major polypeptides of Saccharomyces cerevisiae depending on the fermentable or nonfermentable nature of carbon source
    • Bataillé, N., Thoraval, D. and Boucherie, H. (1988). Two-dimensional gel analysis of yeast proteins: Application to the study of changes in the levels of major polypeptides of Saccharomyces cerevisiae depending on the fermentable or nonfermentable nature of carbon source. Electrophoresis 9, 774-780.
    • (1988) Electrophoresis , vol.9 , pp. 774-780
    • Bataillé, N.1    Thoraval, D.2    Boucherie, H.3
  • 5
    • 0028302337 scopus 로고
    • Reference points for comparisons of two-dimensional maps of proteins from different human cell types defined in a pH scale where isoelectric points correlate with polypeptide compositions
    • Bjellqvist, B., Basse, B., Olsen, E. and Celis. J. E. (1994). Reference points for comparisons of two-dimensional maps of proteins from different human cell types defined in a pH scale where isoelectric points correlate with polypeptide compositions. Electrophoresis 15, 529-539.
    • (1994) Electrophoresis , vol.15 , pp. 529-539
    • Bjellqvist, B.1    Basse, B.2    Olsen, E.3    Celis, J.E.4
  • 6
    • 0027521381 scopus 로고
    • The focusing position of polypeptides in immobilized pH gradients can be predicted from their amino acid sequences
    • Bjellqvist, B., Hughes, G. J., Pasquali, C., et al. (1993). The focusing position of polypeptides in immobilized pH gradients can be predicted from their amino acid sequences. Electrophoresis 14, 1023-1031.
    • (1993) Electrophoresis , vol.14 , pp. 1023-1031
    • Bjellqvist, B.1    Hughes, G.J.2    Pasquali, C.3
  • 7
    • 0029054221 scopus 로고
    • Global changes in protein synthesis during adaptation to 0.7 M-NaCl medium of Saccharomyces cerevisiae
    • Blomberg, A. (1995). Global changes in protein synthesis during adaptation to 0.7 M-NaCl medium of Saccharomyces cerevisiae. J. Bacteriol. 177, 3563-3572.
    • (1995) J. Bacteriol. , vol.177 , pp. 3563-3572
    • Blomberg, A.1
  • 8
    • 0028868712 scopus 로고
    • Interlaboratory reproducibility of yeast protein patterns analyzed by immobilised pH gradient two-dimensional electrophoresis
    • Blomberg, A., Blomberg, L., Norbeck, J., et al. (1995). Interlaboratory reproducibility of yeast protein patterns analyzed by immobilised pH gradient two-dimensional electrophoresis. Electrophoresis 16, 1935-1945.
    • (1995) Electrophoresis , vol.16 , pp. 1935-1945
    • Blomberg, A.1    Blomberg, L.2    Norbeck, J.3
  • 10
    • 0021913732 scopus 로고
    • Protein synthesis during transition and stationary phases under glucose limitation in Saccharomyes cerevisiae
    • Boucherie, H. (1985). Protein synthesis during transition and stationary phases under glucose limitation in Saccharomyes cerevisiae. J. Bacteriol. 161, 385-392.
    • (1985) J. Bacteriol. , vol.161 , pp. 385-392
    • Boucherie, H.1
  • 11
    • 0028985640 scopus 로고
    • Differential synthesis of glyceraldehyde-3-phosphate dehydrogenase polypeptides in stressed yeast cells
    • Boucherie, H., Bataillé, N., Fitch, I. T., Perrot, M. and Tuite, M. F. (1995a). Differential synthesis of glyceraldehyde-3-phosphate dehydrogenase polypeptides in stressed yeast cells. FEMS Microbiol. Lett. 125, 127-134.
    • (1995) FEMS Microbiol. Lett. , vol.125 , pp. 127-134
    • Boucherie, H.1    Bataillé, N.2    Fitch, I.T.3    Perrot, M.4    Tuite, M.F.5
  • 12
    • 0029024531 scopus 로고
    • Two-dimensional protein map of Saccharomyces cerevisiae: Construction of a gene-protein index
    • Boucherie, H., Dujardin, G., Kermorgant, M., Monribot, C, Slonimski, P. and Perrot, M. (1995b). Two-dimensional protein map of Saccharomyces cerevisiae: construction of a gene-protein index. Yeast 11, 601-613.
    • (1995) Yeast , vol.11 , pp. 601-613
    • Boucherie, H.1    Dujardin, G.2    Kermorgant, M.3    Monribot, C.4    Slonimski, P.5    Perrot, M.6
  • 13
    • 0029144085 scopus 로고
    • Challenging times for bioinformatics
    • Casari, G., Andrade, M., Bork, P., et al. (1995). Challenging times for bioinformatics. Nature 376, 647-648.
    • (1995) Nature , vol.376 , pp. 647-648
    • Casari, G.1    Andrade, M.2    Bork, P.3
  • 14
    • 0029897603 scopus 로고    scopus 로고
    • Bioinformatics and the discovery of gene function
    • Casari, G., Daruvar, A., Sanders, C. and Schnider, R. (1996). Bioinformatics and the discovery of gene function. TIGS, 12, 244-245.
    • (1996) TIGS , vol.12 , pp. 244-245
    • Casari, G.1    Daruvar, A.2    Sanders, C.3    Schnider, R.4
  • 15
    • 0027992574 scopus 로고
    • Positional reproducibility of protein spots in two-dimensional polyacrylamide gel electrophoresis using immobilised pH gradient isoelectric focusing in the first dimension: An interlaboratory comparison
    • Corbett, J. M., Dunn, M. J., Posch, A. and Görg, A. (1994). Positional reproducibility of protein spots in two-dimensional polyacrylamide gel electrophoresis using immobilised pH gradient isoelectric focusing in the first dimension: An interlaboratory comparison. Electrophoresis 15, 1205-1211.
    • (1994) Electrophoresis , vol.15 , pp. 1205-1211
    • Corbett, J.M.1    Dunn, M.J.2    Posch, A.3    Görg, A.4
  • 16
    • 0021763691 scopus 로고
    • Regulation of enzymes and isoenzymes of carbohydrate metabolism in the yeast Saccharomyces cerevisiae
    • Entian, K. D., Fröhlish, K. U. and Mecke, D. (1984). Regulation of enzymes and isoenzymes of carbohydrate metabolism in the yeast Saccharomyces cerevisiae. Biochim. Biophys. Acta 799, 181-186.
    • (1984) Biochim. Biophys. Acta , vol.799 , pp. 181-186
    • Entian, K.D.1    Fröhlish, K.U.2    Mecke, D.3
  • 17
    • 0024978195 scopus 로고
    • The QUEST system for quantitative analysis of two-dimensional gels
    • Garrels, J. I. (1989). The QUEST system for quantitative analysis of two-dimensional gels. J. Biol. Chem. 264, 5269-5282.
    • (1989) J. Biol. Chem. , vol.264 , pp. 5269-5282
    • Garrels, J.I.1
  • 18
    • 0027941628 scopus 로고
    • Protein identifications for a Saccharomyces cerevisiae protein database
    • Garrels, J. I., Futcher, B., Kobayashi, R., et al. (1994). Protein identifications for a Saccharomyces cerevisiae protein database. Electrophoresis 15, 1-21.
    • (1994) Electrophoresis , vol.15 , pp. 1-21
    • Garrels, J.I.1    Futcher, B.2    Kobayashi, R.3
  • 19
    • 4244196827 scopus 로고
    • High-resolution two-dimensional electrophoresis of proteins using immobilized pH gradients
    • (eds), Academic Press, New York
    • Görg, A. (1994). High-resolution two-dimensional electrophoresis of proteins using immobilized pH gradients. In (eds), Cell Biology: A Laboratory Handbook. Academic Press, New York, pp. 231-242.
    • (1994) Cell Biology: A Laboratory Handbook , pp. 231-242
    • Görg, A.1
  • 20
    • 0023768475 scopus 로고
    • The current state of two-dimensional electrophoresis with immobilized pH gradients
    • Görg, A., Postel, W. and Günther, S. (1988). The current state of two-dimensional electrophoresis with immobilized pH gradients. Electrophoresis 9, 531-546.
    • (1988) Electrophoresis , vol.9 , pp. 531-546
    • Görg, A.1    Postel, W.2    Günther, S.3
  • 21
    • 0026462996 scopus 로고
    • Mutations at the putative junction sites of the yeast VMA1 protein, the catalytic subunit of the vacuolar membrane H(+)-ATPase, inhibits its processing by protein splicing. Biochem
    • Hirata, R. and Anruku, Y. (1992). Mutations at the putative junction sites of the yeast VMA1 protein, the catalytic subunit of the vacuolar membrane H(+)-ATPase, inhibits its processing by protein splicing. Biochem. Biophys. Res. Comm. 188, 40-47.
    • (1992) Biophys. Res. Comm. , vol.188 , pp. 40-47
    • Hirata, R.1    Anruku, Y.2
  • 22
    • 0028788811 scopus 로고
    • From genome to proteome: Looking at a cell's proteins
    • Kahn, P. (1995). From genome to proteome: looking at a cell's proteins. Science 270, 369-370.
    • (1995) Science , vol.270 , pp. 369-370
    • Kahn, P.1
  • 24
    • 0016637146 scopus 로고
    • Protein mapping by combined isoelectric focusing and electrophoresis in mouse tissues: A novel approach to testing for induced point mutations in mammals
    • Klose, J. (1975). Protein mapping by combined isoelectric focusing and electrophoresis in mouse tissues: a novel approach to testing for induced point mutations in mammals. Human Genetics 26, 231-243.
    • (1975) Human Genetics , vol.26 , pp. 231-243
    • Klose, J.1
  • 25
    • 0031002521 scopus 로고    scopus 로고
    • Identifications of two-dimensional gel electrophoresis resolved yeast proteins by matrix-assisted laser desorption ionisation mass spectrometry
    • Larsson, T., Norbeck, J., Karlsson, H., Karlsson, K.-A. and Blomberg, A. (1997). Identifications of two-dimensional gel electrophoresis resolved yeast proteins by matrix-assisted laser desorption ionisation mass spectrometry. Electrophoresis 18, 418-423.
    • (1997) Electrophoresis , vol.18 , pp. 418-423
    • Larsson, T.1    Norbeck, J.2    Karlsson, H.3    Karlsson, K.-A.4    Blomberg, A.5
  • 26
    • 0020025107 scopus 로고
    • Synthesis of specific identified, phosphorylated, heat shock, and heal stroke proteins through the cell cycle of Saccharomyces cerevisiae
    • Ludwig, J. R., Foy, J. J., Elliot, S. G. and McLaughlin, C. S. (1982). Synthesis of specific identified, phosphorylated, heat shock, and heal stroke proteins through the cell cycle of Saccharomyces cerevisiae. Mol Cell Biol. 2, 117-126.
    • (1982) Mol Cell Biol. , vol.2 , pp. 117-126
    • Ludwig, J.R.1    Foy, J.J.2    Elliot, S.G.3    McLaughlin, C.S.4
  • 27
    • 0022385984 scopus 로고
    • Differential expression of the three yeast glyceraldehyde-3-phosphate dehydrogenase genes
    • McAlister, L. and Holland, M. J. (1985). Differential expression of the three yeast glyceraldehyde-3-phosphate dehydrogenase genes. J. Biol. Chem. 260, 15019-15027.
    • (1985) J. Biol. Chem. , vol.260 , pp. 15019-15027
    • McAlister, L.1    Holland, M.J.2
  • 28
    • 15444354989 scopus 로고
    • Cloning and molecular characterisation of three genes, including two genes encoding serine hydroxymethyltransferases, whose inactivation is required to render yeast auxotrophic for glycine
    • McNeil, J. B., McIntosh, E. M., Taylor, B. V., Zhang, F. R., Tang, S. and Bognar, A. L. (1994). Cloning and molecular characterisation of three genes, including two genes encoding serine hydroxymethyltransferases, whose inactivation is required to render yeast auxotrophic for glycine. Mol. Biol. Cell 3, 29-47.
    • (1994) Mol. Biol. Cell , vol.3 , pp. 29-47
    • McNeil, J.B.1    McIntosh, E.M.2    Taylor, B.V.3    Zhang, F.R.4    Tang, S.5    Bognar, A.L.6
  • 29
    • 0020264211 scopus 로고
    • Quantitative analysis of heat shock response of Saccharomyces cerevisiae
    • Miller, M. J., Xuong, N.-H. and Geiduschek. E. P. (1982). Quantitative analysis of heat shock response of Saccharomyces cerevisiae. J. Bacteriol. 151, 311-327.
    • (1982) J. Bacteriol. , vol.151 , pp. 311-327
    • Miller, M.J.1    Xuong, N.-H.2    Geiduschek, E.P.3
  • 30
    • 0026736113 scopus 로고
    • Yeast actin is relatively well behaved
    • Nefsky, B. and Bretscher, A. (1992). Yeast actin is relatively well behaved. Eu. J. Biochem. 206, 949-955.
    • (1992) Eu. J. Biochem. , vol.206 , pp. 949-955
    • Nefsky, B.1    Bretscher, A.2
  • 31
    • 0026649409 scopus 로고
    • The translational machinery and 70 kd heat shock protein cooperate in protein synthesis
    • Nelson, R. J., Ziegelhoffer, T., Nicolet, C., Werner-Washburne, M. and Craig, E. A. (1992). The translational machinery and 70 kd heat shock protein cooperate in protein synthesis. Cell 71, 97-105.
    • (1992) Cell , vol.71 , pp. 97-105
    • Nelson, R.J.1    Ziegelhoffer, T.2    Nicolet, C.3    Werner-Washburne, M.4    Craig, E.A.5
  • 32
    • 0029124186 scopus 로고
    • Differential gene expression in the murine thymus assayed by quantitative hybridisation of arrayed cDNA clones
    • Nguyen, C., Rocha, D., Grunjeaud, S., et al. (1995). Differential gene expression in the murine thymus assayed by quantitative hybridisation of arrayed cDNA clones. Genomics 29, 207-216.
    • (1995) Genomics , vol.29 , pp. 207-216
    • Nguyen, C.1    Rocha, D.2    Grunjeaud, S.3
  • 34
    • 0028797968 scopus 로고
    • Gene linkage of two-dimensional polyacrylamide gel electrophoresis resolved proteins from isogene families in Saccharomyes cerevisiae by microsequencing of in-gel trypsin generated peptides
    • Norbeck, J. and Blomberg, A. (1995). Gene linkage of two-dimensional polyacrylamide gel electrophoresis resolved proteins from isogene families in Saccharomyes cerevisiae by microsequencing of in-gel trypsin generated peptides. Electrophoresis 16, 149-156.
    • (1995) Electrophoresis , vol.16 , pp. 149-156
    • Norbeck, J.1    Blomberg, A.2
  • 35
    • 0029990019 scopus 로고    scopus 로고
    • Protein expression during exponential growth in 0.7 M-NaCl medium of Saccharomyces cerevisiae
    • Norbeck, J. and Blomberg, A. (1996). Protein expression during exponential growth in 0.7 M-NaCl medium of Saccharomyces cerevisiae. FEMS Microbiol. Lett. 137, 1-8.
    • (1996) FEMS Microbiol. Lett. , vol.137 , pp. 1-8
    • Norbeck, J.1    Blomberg, A.2
  • 36
    • 0031041461 scopus 로고    scopus 로고
    • Metabolic and regulatory changes associated with growth of Saccharomyces cerevisiae in 1-4 M-sodium chloride: Evidence for osmotic induction of glycerol dissimilation via the dihydroxyacetone pathway
    • Norbeck, J. and Blomberg, A. (1997). Metabolic and regulatory changes associated with growth of Saccharomyces cerevisiae in 1-4 M-sodium chloride: Evidence for osmotic induction of glycerol dissimilation via the dihydroxyacetone pathway. J. Biol. Chem. 272 5544-5554.
    • (1997) J. Biol. Chem. , vol.272 , pp. 5544-5554
    • Norbeck, J.1    Blomberg, A.2
  • 37
    • 0029920291 scopus 로고    scopus 로고
    • Purification and characterization of two isoenzymes of DL-glycerol 3-phosphatase from Saccharomyces cerevisiae. Identification of the corresponding GPP1 and GPP2 genes and evidence for osmotic regulation of Gpp2p expression by the osmosensing mitogen-activated protein kinase signal transduction pathway
    • Norbeck, J., Påhlmann, A.-K., Akhtar, N., Blomberg, A. and Adler, L. (1996), Purification and characterization of two isoenzymes of DL-glycerol 3-phosphatase from Saccharomyces cerevisiae. Identification of the corresponding GPP1 and GPP2 genes and evidence for osmotic regulation of Gpp2p expression by the osmosensing mitogen-activated protein kinase signal transduction pathway. J. Biol. Chem 271, 13843-13875.
    • (1996) J. Biol. Chem , vol.271 , pp. 13843-13875
    • Norbeck, J.1    Påhlmann, A.-K.2    Akhtar, N.3    Blomberg, A.4    Adler, L.5
  • 38
    • 0016711037 scopus 로고
    • High resolution two-dimensional electrophoresis of proteins
    • O'Farrell, P. H. (1975). High resolution two-dimensional electrophoresis of proteins. J. Biol. Chem. 250, 4007-4021.
    • (1975) J. Biol. Chem. , vol.250 , pp. 4007-4021
    • O'Farrell, P.H.1
  • 39
    • 0017696571 scopus 로고
    • High resolution two-dimensional electrophoresis of basic as well as acidic proteins
    • O'Farrell, P. Z., Goodman, H. M. and O'Farrell, P. H. (1977). High resolution two-dimensional electrophoresis of basic as well as acidic proteins. Cell 12, 1133-1142.
    • (1977) Cell , vol.12 , pp. 1133-1142
    • O'Farrell, P.Z.1    Goodman, H.M.2    O'Farrell, P.H.3
  • 40
    • 0030071902 scopus 로고    scopus 로고
    • From DNA sequence to biological function
    • Oliver, G. S. (1996). From DNA sequence to biological function. Nature 379, 597-600.
    • (1996) Nature , vol.379 , pp. 597-600
    • Oliver, G.S.1
  • 41
    • 0030957650 scopus 로고    scopus 로고
    • Improvements of the solubilisation of proteins in two-dimensional electrophoresis with immobilised pH gradients
    • Rabilloud, T., Adessi, C, Giraudel, A. and Lunardi, J. (1997). Improvements of the solubilisation of proteins in two-dimensional electrophoresis with immobilised pH gradients. Electrophoresis 18, 307-316.
    • (1997) Electrophoresis , vol.18 , pp. 307-316
    • Rabilloud, T.1    Adessi, C.2    Giraudel, A.3    Lunardi, J.4
  • 42
    • 0029972156 scopus 로고    scopus 로고
    • Identifications of proteins of the yeast protein map using genetically manipulated strains and peptide-mass fingerprinting
    • Sagliocco, F., Guillemot, J.-C., Monribot, C, et al. (1996). Identifications of proteins of the yeast protein map using genetically manipulated strains and peptide-mass fingerprinting. Yeast 12, 1519-1533.
    • (1996) Yeast , vol.12 , pp. 1519-1533
    • Sagliocco, F.1    Guillemot, J.-C.2    Monribot, C.3
  • 43
    • 10544244161 scopus 로고    scopus 로고
    • Linking genome and proteome by mass spectrometry: Large-scale identification of yeast proteins from two dimensional gels
    • Shevchenko, A., Jensen, O. N., Podtelejnikov, A. V., et al. (1996). Linking genome and proteome by mass spectrometry: large-scale identification of yeast proteins from two dimensional gels. Proc. Natl. Acad. Sci. USA. 93, 14440-14445.
    • (1996) Proc. Natl. Acad. Sci. USA , vol.93 , pp. 14440-14445
    • Shevchenko, A.1    Jensen, O.N.2    Podtelejnikov, A.V.3
  • 44
    • 0031442518 scopus 로고    scopus 로고
    • Characterization of the yeast transcriptome
    • Valculescu, V. E., Zhang, L., Zhou, W., et al. (1997). Characterization of the yeast transcriptome. Cell 88, 243-251.
    • (1997) Cell , vol.88 , pp. 243-251
    • Valculescu, V.E.1    Zhang, L.2    Zhou, W.3


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.