Formation of β-galactosides of pyridoxine using Sporobolomyces singularis

Methods in Enzymology

Volumn 280, Issue , 1997, Pages 71-77

  Suzuki, Yukio ^a   Uchida, Kei ^a  

^a NONE

Author keywords

[No Author keywords available]

Indexed keywords

BETA GALACTOSIDE; PYRIDOXINE DERIVATIVE;

ARTICLE; CHEMICAL REACTION; CULTURE MEDIUM; FERMENTATION; FUNGUS CULTURE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; NONHUMAN; PRIORITY JOURNAL; YEAST;

FUNGI; SPOROBOLOMYCES SINGULARIS;

EID: 0031420692     PISSN: 00766879     EISSN: None     Source Type: Book Series    
DOI: 10.1016/S0076-6879(97)80103-6     Document Type: Article

References (5)

1. Y. Suzuki and K. Uchida, Biochim. Biophys. Acta 1116, 67 (1992).
2. K. Ogata, Y. Tani, Y. Uchida, and T. Tochikura, J. Vitaminol. 15, 142 (1969).
3. N. Nelson, J. Biol. Chem. 153, 375 (1944).
4. Y. Suzuki, K. Uchida, and A. Tsuhoi, Nippon Nogeikagaku Kaishi [J. Agric. Chem. Soc. Jpn.] 53, 189 (1979). [In Japanese]
5. Reaction mixture containing 500 mg of lactose, 206 mg of pyridoxine hydrochloride (neutralized to pH 7.0), 5 ml of Mcllvaine's buffer (pH 3.8-8.0), 1.3 ml of cell suspension (127 mg of cells as dry weight), and deionized water to a final volume of 10 ml is incubated at 25° for 18 hr in the dark while shaking.