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Volumn 33, Issue 10, 1997, Pages 774-782

Development of embryonic chick insulin cells in culture: Beneficial effects of serum-free medium, raised nutrients, and biomatrix

Author keywords

Culture media Serum free; Differentiation; Extracellular matrix; Organ culture; Pancreatic beta cells

Indexed keywords

AMINO ACID; COLLAGEN GEL; GLUCAGON; GLUCOSE; INSULIN; MATRIGEL;

EID: 0031417019     PISSN: 10712690     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11626-997-0156-5     Document Type: Article
Times cited : (13)

References (50)
  • 1
    • 0023949396 scopus 로고
    • Hybrid insulin genes reveal a developmental lineage for pancreatic endocrine cells and imply a relationship with neurons
    • Alpert, S.; Hanahan, D.; Teitelman, G. Hybrid insulin genes reveal a developmental lineage for pancreatic endocrine cells and imply a relationship with neurons. Cell 53:295-308; 1988.
    • (1988) Cell , vol.53 , pp. 295-308
    • Alpert, S.1    Hanahan, D.2    Teitelman, G.3
  • 2
    • 0018189864 scopus 로고
    • Isolated mouse pancreatic islets in culture: Effects of serum and different culture media on the insulin production of the islets
    • Andersson, A. Isolated mouse pancreatic islets in culture: effects of serum and different culture media on the insulin production of the islets. Diabetologia 14:397-404; 1978.
    • (1978) Diabetologia , vol.14 , pp. 397-404
    • Andersson, A.1
  • 3
    • 0021611867 scopus 로고
    • The development of the gastro-entero-pancreatic neuroendocrine system in birds
    • Falkmer, S.; Håkanson, R.; Sundler, F., eds. Amsterdam, Netherlands: Elsevier Science Publishers BV;
    • Andrew, A. The development of the gastro-entero-pancreatic neuroendocrine system in birds. In: Falkmer, S.; Håkanson, R.; Sundler, F., eds. Evolution and tumour pathology of the neuroendocrine system. Amsterdam, Netherlands: Elsevier Science Publishers BV; 1984:91-109.
    • (1984) Evolution and Tumour Pathology of the Neuroendocrine System , pp. 91-109
    • Andrew, A.1
  • 4
    • 0028153456 scopus 로고
    • Failure of insulin cells to develop in cultured embryonic chick pancreas: A model system for the detection of factors supporting insulin cell differentiation
    • Andrew, A.; Rawdon, B. B.; Alison, B. C. Failure of insulin cells to develop in cultured embryonic chick pancreas: a model system for the detection of factors supporting insulin cell differentiation. In Vitro Cell. Dev. Biol. 30A:664-670; 1994.
    • (1994) In Vitro Cell. Dev. Biol. , vol.30 A , pp. 664-670
    • Andrew, A.1    Rawdon, B.B.2    Alison, B.C.3
  • 5
    • 0019170218 scopus 로고
    • Serum-free cell culture: A unifying approach
    • Barnes, D.; Sato, G. Serum-free cell culture: a unifying approach. Cell 22:649-655; 1980.
    • (1980) Cell , vol.22 , pp. 649-655
    • Barnes, D.1    Sato, G.2
  • 6
    • 0025912130 scopus 로고
    • Functional impact of attachment and purification in the short term culture of human pancreatic islets
    • Beattie, G. M.; Lappi, D. A.; Hayek, A. Functional impact of attachment and purification in the short term culture of human pancreatic islets. J. Clin. Endocrinol. & Metab. 73:93-98; 1991.
    • (1991) J. Clin. Endocrinol. & Metab. , vol.73 , pp. 93-98
    • Beattie, G.M.1    Lappi, D.A.2    Hayek, A.3
  • 7
    • 0016267447 scopus 로고
    • Functional differentiation of the chick endocrine pancreas: Insulin storage and secretion
    • Benzo, C. A.; Green, T. D. Functional differentiation of the chick endocrine pancreas: insulin storage and secretion. Anat. Rec. 180:491-496; 1974.
    • (1974) Anat. Rec. , vol.180 , pp. 491-496
    • Benzo, C.A.1    Green, T.D.2
  • 8
    • 0018320872 scopus 로고
    • The growth of cells in serum-free hormone-supplemented media
    • Jakoby, W. B.; Pastan, I. H., eds. New York: Academic Press
    • Bottenstein, J.; Hayashi, I.; Hutchings, S., et al. The growth of cells in serum-free hormone-supplemented media. In: Jakoby, W. B.; Pastan, I. H., eds. Methods in enzymology. Vol. 58. New York: Academic Press; 1979:94-109.
    • (1979) Methods in Enzymology , vol.58 , pp. 94-109
    • Bottenstein, J.1    Hayashi, I.2    Hutchings, S.3
  • 9
    • 0013931415 scopus 로고
    • Postnatal neogenesis of islets of Langerhans in the mouse
    • Bunnag, S. C. Postnatal neogenesis of islets of Langerhans in the mouse. Diabetes 15:480-491; 1966.
    • (1966) Diabetes , vol.15 , pp. 480-491
    • Bunnag, S.C.1
  • 10
    • 0017875968 scopus 로고
    • Effect of glucose and amino acids on foetal rat pancreatic growth and insulin secretion in vitro
    • de Gasparo, M.; Milner, G. R.; Norris, P. D., et al. Effect of glucose and amino acids on foetal rat pancreatic growth and insulin secretion in vitro. J. Endocrinol. 77:241-248; 1978.
    • (1978) J. Endocrinol. , vol.77 , pp. 241-248
    • De Gasparo, M.1    Milner, G.R.2    Norris, P.D.3
  • 11
    • 0002189305 scopus 로고
    • The comparative physiology of the pancreatic islets
    • Berlin, Germany: Springer-Verlag
    • Epple, A.; Brinn, J. E. The comparative physiology of the pancreatic islets. Zoophysiology 21. Berlin, Germany: Springer-Verlag; 1987:94.
    • (1987) Zoophysiology , vol.21 , pp. 94
    • Epple, A.1    Brinn, J.E.2
  • 12
    • 0019990137 scopus 로고
    • Stimulation of insulin and glucagon secretion in organ culture of chick endocrine pancreas during embryonic life and after hatching
    • Foltzer, C.; Haffen, K.; Kedinger, M., et al. Stimulation of insulin and glucagon secretion in organ culture of chick endocrine pancreas during embryonic life and after hatching. Gen. Comp. Endocrinol. 47:213-220; 1982.
    • (1982) Gen. Comp. Endocrinol. , vol.47 , pp. 213-220
    • Foltzer, C.1    Haffen, K.2    Kedinger, M.3
  • 13
    • 0019757926 scopus 로고
    • Hormones and factors that stimulate growth of a rat islet tumour cell line in serum-free medium
    • Fong, H. K. W.; Chick, W. L.; Sato, G. H. Hormones and factors that stimulate growth of a rat islet tumour cell line in serum-free medium. Diabetes 30:1022-1028; 1981.
    • (1981) Diabetes , vol.30 , pp. 1022-1028
    • Fong, H.K.W.1    Chick, W.L.2    Sato, G.H.3
  • 14
    • 0030041280 scopus 로고    scopus 로고
    • Lineage-specific morphogenesis in the developing pancreas: Role of mesenchymal factors
    • Gittes, G. K.; Galante, P. E.; Hanahan, D., et al. Lineage-specific morphogenesis in the developing pancreas: role of mesenchymal factors. Development 122:439-447; 1996.
    • (1996) Development , vol.122 , pp. 439-447
    • Gittes, G.K.1    Galante, P.E.2    Hanahan, D.3
  • 15
    • 84883833623 scopus 로고
    • Clonal growth of mammalian cells in a chemically defined, synthetic medium
    • Ham, R. Clonal growth of mammalian cells in a chemically defined, synthetic medium. Proc. Natl. Acad. Sci. USA 53:288-293; 1965.
    • (1965) Proc. Natl. Acad. Sci. USA , vol.53 , pp. 288-293
    • Ham, R.1
  • 16
    • 0018310017 scopus 로고
    • Media and growth requirements
    • Jakoby, W. B.; Pastan, I. H., eds. New York: Academic Press
    • Ham, R. G.; McKeehan, W. L. Media and growth requirements. In: Jakoby, W. B.; Pastan, I. H., eds. Methods in enzymology. Vol. 58. New York: Academic Press; 1979:44-93.
    • (1979) Methods in Enzymology , vol.58 , pp. 44-93
    • Ham, R.G.1    McKeehan, W.L.2
  • 17
    • 84924926133 scopus 로고
    • A series of normal stages in the development of the chick embryo
    • Hamburger, V.; Hamilton, H. L. A series of normal stages in the development of the chick embryo. J. Morphol. 88:49-92; 1951.
    • (1951) J. Morphol. , vol.88 , pp. 49-92
    • Hamburger, V.1    Hamilton, H.L.2
  • 18
    • 0024598735 scopus 로고
    • Enhancement of pancreatic islet cell monolayer growth by endothelial cell matrix and insulin
    • Hayek, A.; Lopez, A. D.; Beattie, G. M. Enhancement of pancreatic islet cell monolayer growth by endothelial cell matrix and insulin. In Vitro Cell. Dev. Biol. 25:146-150; 1989.
    • (1989) In Vitro Cell. Dev. Biol. , vol.25 , pp. 146-150
    • Hayek, A.1    Lopez, A.D.2    Beattie, G.M.3
  • 19
    • 0029793616 scopus 로고    scopus 로고
    • PYY in developing murine islet cells: Comparisons to development of islet hormones, NPY, and BrdU incorporation
    • Jackerott, M.; Øster, A.; Larsson, L.-I. PYY in developing murine islet cells: comparisons to development of islet hormones, NPY, and BrdU incorporation. J. Histochem. Cytochem. 44:809-317; 1996.
    • (1996) J. Histochem. Cytochem. , vol.44 , pp. 809-1317
    • Jackerott, M.1    Øster, A.2    Larsson, L.-I.3
  • 20
    • 0027160032 scopus 로고
    • Extracellular matrix contains insulin-like growth factor binding protein-5 - Potentiation of the effects of IGF-I
    • Jones, J. I.; Gockerman, A.; Busby, W. H., et al. Extracellular matrix contains insulin-like growth factor binding protein-5 - potentiation of the effects of IGF-I. J. Cell Biol. 121:679-687; 1993.
    • (1993) J. Cell Biol. , vol.121 , pp. 679-687
    • Jones, J.I.1    Gockerman, A.2    Busby, W.H.3
  • 21
    • 0016425026 scopus 로고
    • Development of response to glucose of foetal rat islets in organ culture
    • Kaung, H.-L. C.; Hegre, O. D.; Lazarow, A. Development of response to glucose of foetal rat islets in organ culture. Proc. Soc. Exp. Biol. Med. 148:75-79; 1975.
    • (1975) Proc. Soc. Exp. Biol. Med. , vol.148 , pp. 75-79
    • Kaung, H.-L.C.1    Hegre, O.D.2    Lazarow, A.3
  • 22
    • 0025668782 scopus 로고
    • Culture of endocrine pancreatic cells in protein-free, chemically defined media
    • Kinard, F.; de Clercq, L.; Billen, B., et al. Culture of endocrine pancreatic cells in protein-free, chemically defined media. In Vitro Cell. Dev. Biol. 26:1004-1010; 1990.
    • (1990) In Vitro Cell. Dev. Biol. , vol.26 , pp. 1004-1010
    • Kinard, F.1    De Clercq, L.2    Billen, B.3
  • 23
    • 0028334897 scopus 로고
    • Endocrine and mucous dilferentiation by a cloned human rectal adenocarcinoma cell line (HRA-19) in vitro: Inhibition by TGF-β1
    • Kirkland, S. C.; Henderson, K. Endocrine and mucous dilferentiation by a cloned human rectal adenocarcinoma cell line (HRA-19) in vitro: inhibition by TGF-β1. J. Cell Sci. 107:1041-1046; 1994.
    • (1994) J. Cell Sci. , vol.107 , pp. 1041-1046
    • Kirkland, S.C.1    Henderson, K.2
  • 24
    • 0016524491 scopus 로고
    • The pancreatic insulin content and its relationship to plasma glucose and free fatty acid concentrations in the embryo and neonatal chick
    • Langslow, D. R. The pancreatic insulin content and its relationship to plasma glucose and free fatty acid concentrations in the embryo and neonatal chick. Br. Poult. Sci. 16:329-333; 1975.
    • (1975) Br. Poult. Sci. , vol.16 , pp. 329-333
    • Langslow, D.R.1
  • 25
    • 0007911551 scopus 로고
    • Non-radioactive in situ mRNA hybridisation using synthetic oligonucleotides: Principles, combination with immunocytochemistry and quantitation
    • Larsson, L.-I.; Hougaard, D. M. Non-radioactive in situ mRNA hybridisation using synthetic oligonucleotides: principles, combination with immunocytochemistry and quantitation. Neurosci. Protocols 20:1-18; 1993.
    • (1993) Neurosci. Protocols , vol.20 , pp. 1-18
    • Larsson, L.-I.1    Hougaard, D.M.2
  • 26
    • 0028084965 scopus 로고
    • Effect of nutrients, hormones and serum on survival of rat islet beta cells in culture
    • Ling, Z.; Hannaert, J. C.; Pipeleers, D. Effect of nutrients, hormones and serum on survival of rat islet beta cells in culture. Diabetologia 37:15-21; 1994.
    • (1994) Diabetologia , vol.37 , pp. 15-21
    • Ling, Z.1    Hannaert, J.C.2    Pipeleers, D.3
  • 27
    • 0019174946 scopus 로고
    • Fetal rat pancreas in organ culture: Effects of serum on the development of the endocrine cells
    • McEvoy, R. C. Fetal rat pancreas in organ culture: effects of serum on the development of the endocrine cells. Differentiation 17:105-109; 1980.
    • (1980) Differentiation , vol.17 , pp. 105-109
    • McEvoy, R.C.1
  • 28
    • 0020216238 scopus 로고
    • Tissue culture of fetal rat islets: Comparison of serum-supplemented and serum-free, defined medium on the maintenance, growth and differentiation of A, B and D cells
    • McEvoy, R.; Leung, P. E. Tissue culture of fetal rat islets: comparison of serum-supplemented and serum-free, defined medium on the maintenance, growth and differentiation of A, B and D cells. Endocrinology 111:1568-1575; 1982.
    • (1982) Endocrinology , vol.111 , pp. 1568-1575
    • McEvoy, R.1    Leung, P.E.2
  • 29
    • 0022645146 scopus 로고
    • Regulation of insulin mRNA abundance and adenylation: Dependence on hormones and matrix substrata
    • Muschel, R.; Khoury, G.; Reid, L. M. Regulation of insulin mRNA abundance and adenylation: dependence on hormones and matrix substrata. Mol. Cell. Biol. 6:337-341; 1986.
    • (1986) Mol. Cell. Biol. , vol.6 , pp. 337-341
    • Muschel, R.1    Khoury, G.2    Reid, L.M.3
  • 30
    • 0014078755 scopus 로고
    • The morphogenesis of the pancreas in the chicken
    • Ono, K. The morphogenesis of the pancreas in the chicken. Acta. Anat. Nippon. 42:99-109; 1967.
    • (1967) Acta. Anat. Nippon. , vol.42 , pp. 99-109
    • Ono, K.1
  • 31
    • 0003345999 scopus 로고
    • Histochemistry
    • London: Churchill
    • Pearse, A. G. E. Histochemistry. Theoretical and applied. Vol. 1. London: Churchill; 1968:601.
    • (1968) Theoretical and Applied , vol.1 , pp. 601
    • Pearse, A.G.E.1
  • 32
    • 0016639704 scopus 로고
    • Bifunctional reagents as vapour and liquid phase fixatives for immunohistochemistry
    • Pearse, A. G. E.; Polak, J. M. Bifunctional reagents as vapour and liquid phase fixatives for immunohistochemistry. Histochem. J. 7:179-186; 1975.
    • (1975) Histochem. J. , vol.7 , pp. 179-186
    • Pearse, A.G.E.1    Polak, J.M.2
  • 33
    • 0018857156 scopus 로고
    • The evolution of genes: The chicken preproinsulin gene
    • Perler, F.; Efstratiadis, A.; Lomedico, P., et al. The evolution of genes: the chicken preproinsulin gene. Cell 20:555-566; 1980.
    • (1980) Cell , vol.20 , pp. 555-566
    • Perler, F.1    Efstratiadis, A.2    Lomedico, P.3
  • 34
    • 0024411191 scopus 로고
    • Proliferation of immunocytochemically identified islet beta cells in culture: Effect of growth factors, hormones and nutrients
    • Popiela, H.; Moore, W. Proliferation of immunocytochemically identified islet beta cells in culture: effect of growth factors, hormones and nutrients. Pancreas 4:244-248; 1989.
    • (1989) Pancreas , vol.4 , pp. 244-248
    • Popiela, H.1    Moore, W.2
  • 35
    • 0020067003 scopus 로고
    • Insulin and multiplication stimulating activity (an insulin-like growth factor) stimulate islet β-cell replication in neonatal rat pancreatic monolayer cultures
    • Rabinovitch, A.; Quigley, C.; Russel, T., et al. Insulin and multiplication stimulating activity (an insulin-like growth factor) stimulate islet β-cell replication in neonatal rat pancreatic monolayer cultures. Diabetes 31:160-164; 1982.
    • (1982) Diabetes , vol.31 , pp. 160-164
    • Rabinovitch, A.1    Quigley, C.2    Russel, T.3
  • 36
    • 0018216539 scopus 로고
    • Preventing evaporation of antiserum during indirect immunocytochemical staining
    • Rawdon, B. B. Preventing evaporation of antiserum during indirect immunocytochemical staining. Stain Technol. 53:289-290; 1978.
    • (1978) Stain Technol. , vol.53 , pp. 289-290
    • Rawdon, B.B.1
  • 37
    • 0018355406 scopus 로고
    • An immunocytochemical study of the distribution of pancreatic endocrine cells in chicks, with special reference to the relationship between pancreatic polypeptide- and somatostatin-immunoreactive cells
    • Rawdon, B. B.; Andrew, A. An immunocytochemical study of the distribution of pancreatic endocrine cells in chicks, with special reference to the relationship between pancreatic polypeptide- and somatostatin-immunoreactive cells. Histochemistry 59:189-197; 1979.
    • (1979) Histochemistry , vol.59 , pp. 189-197
    • Rawdon, B.B.1    Andrew, A.2
  • 38
    • 0019789767 scopus 로고
    • An immunocytochemical survey of endocrine cells in the gastrointestinal tract of chicks at hatching
    • Rawdon, B. B.; Andrew, A. An immunocytochemical survey of endocrine cells in the gastrointestinal tract of chicks at hatching. Cell Tissue Res. 220:279-292; 1981.
    • (1981) Cell Tissue Res. , vol.220 , pp. 279-292
    • Rawdon, B.B.1    Andrew, A.2
  • 39
    • 0002993285 scopus 로고
    • Cell culture studies using extracts of extracellular matrix to study growth and differentiation in mammalian cells
    • Mather, J. P., ed. New York: Plenum Press
    • Reid, L. M.; Jefferson, D. M. Cell culture studies using extracts of extracellular matrix to study growth and differentiation in mammalian cells. In: Mather, J. P., ed. Mammalian cell culture: the use of serum-free hormone-supplemented media. New York: Plenum Press; 1984:239-280.
    • (1984) Mammalian Cell Culture: The Use of Serum-free Hormone-supplemented Media , pp. 239-280
    • Reid, L.M.1    Jefferson, D.M.2
  • 40
    • 0342738567 scopus 로고
    • Insulin secretion in birds
    • Leclercq, B.; Whitehead, C. C., eds. Great Britain: Butterworths
    • Rideau, N. Insulin secretion in birds. In: Leclercq, B.; Whitehead, C. C., eds. Leanness in domestic birds: genetic, metabolic and hormonal aspects. Great Britain: Butterworths; 1987:269-294.
    • (1987) Leanness in Domestic Birds: Genetic, Metabolic and Hormonal Aspects , pp. 269-294
    • Rideau, N.1
  • 41
    • 0023005762 scopus 로고
    • Further characterisation of insulin secretion from the perfused duodenum-pancreas of chicken: A comparison of insulin release in chickens selected for high and low abdominal fat content
    • Rideau, N.; Simon, J.; Leclercq, B. Further characterisation of insulin secretion from the perfused duodenum-pancreas of chicken: a comparison of insulin release in chickens selected for high and low abdominal fat content. Endocrinology 119:2635-2641; 1986.
    • (1986) Endocrinology , vol.119 , pp. 2635-2641
    • Rideau, N.1    Simon, J.2    Leclercq, B.3
  • 42
    • 0028172289 scopus 로고
    • TGF-beta 1 influences the relative development of the exocrine and endocrine pancreas in vitro
    • Sanvito, F.; Herrera, P.-L.; Huarte, J., et al. TGF-beta 1 influences the relative development of the exocrine and endocrine pancreas In vitro. Development 120:3451-3462; 1994.
    • (1994) Development , vol.120 , pp. 3451-3462
    • Sanvito, F.1    Herrera, P.-L.2    Huarte, J.3
  • 43
    • 0020434321 scopus 로고
    • Longitudinal study of adiposity in chickens selected for high or low abdominal fat content: Further evidence of a glucose-insulin imbalance in the fat line
    • Simon, J.; Leclercq, B. Longitudinal study of adiposity in chickens selected for high or low abdominal fat content: further evidence of a glucose-insulin imbalance in the fat line. J. Nutr. 112:1961-1973; 1982.
    • (1982) J. Nutr. , vol.112 , pp. 1961-1973
    • Simon, J.1    Leclercq, B.2
  • 44
    • 0021885273 scopus 로고
    • Glucose-stimulated DNA replication of the pancreatic islets during the development of the rat fetus: Effects of nutrients, growth hormone and tri-iodothyronine
    • Swenne, I. Glucose-stimulated DNA replication of the pancreatic islets during the development of the rat fetus: effects of nutrients, growth hormone and tri-iodothyronine. Diabetes 34:803-807; 1985.
    • (1985) Diabetes , vol.34 , pp. 803-807
    • Swenne, I.1
  • 45
    • 0026515343 scopus 로고
    • Pancreatic beta-cell growth and diabetes mellitus
    • Swenne, I. Pancreatic beta-cell growth and diabetes mellitus. Diabetologia 35:193-201; 1992.
    • (1992) Diabetologia , vol.35 , pp. 193-201
    • Swenne, I.1
  • 46
    • 0019066260 scopus 로고
    • Effects of glucose and amino acids on the biosynthesis of DNA and insulin in fetal rat islets maintained in tissue culture
    • Swenne, I.; Bone, A. J.; Howell, S. L., et al. Effects of glucose and amino acids on the biosynthesis of DNA and insulin in fetal rat islets maintained in tissue culture. Diabetes 29:686-692; 1980.
    • (1980) Diabetes , vol.29 , pp. 686-692
    • Swenne, I.1    Bone, A.J.2    Howell, S.L.3
  • 47
    • 0023870941 scopus 로고
    • Effects of platelet-derived growth factor and somatomedin-C/insulin-like growth factor I on the deoxyribonucleic acid replication of foetal rat islets of Langerhans in tissue culture
    • Swenne, I.; Heldin, C.-H.; Hill, D. J., et al. Effects of platelet-derived growth factor and somatomedin-C/insulin-like growth factor I on the deoxyribonucleic acid replication of foetal rat islets of Langerhans in tissue culture. Endocrinology 122:214-218; 1988.
    • (1988) Endocrinology , vol.122 , pp. 214-218
    • Swenne, I.1    Heldin, C.-H.2    Hill, D.J.3
  • 48
    • 0027308108 scopus 로고
    • Precursor cells of mouse endocrine pancreas coexpress insulin, glucagon and the neuronal proteins tyrosine hydroxylase and neuropeptide Y, but not pancreatic polypeptide
    • Teitelman, G.; Alpert, S.; Polak, J. M., et al. Precursor cells of mouse endocrine pancreas coexpress insulin, glucagon and the neuronal proteins tyrosine hydroxylase and neuropeptide Y, but not pancreatic polypeptide. Development 118:1031-1039; 1993.
    • (1993) Development , vol.118 , pp. 1031-1039
    • Teitelman, G.1    Alpert, S.2    Polak, J.M.3
  • 49
    • 0027952880 scopus 로고
    • Expression of peptide YY in all four islet cell types in the developing mouse pancreas suggests a common peptide YY-producing progenitor
    • Upchurch, B. H.; Aponte, G. W.; Leiter, A. B. Expression of peptide YY in all four islet cell types in the developing mouse pancreas suggests a common peptide YY-producing progenitor. Development 120:245-252; 1994.
    • (1994) Development , vol.120 , pp. 245-252
    • Upchurch, B.H.1    Aponte, G.W.2    Leiter, A.B.3
  • 50
    • 0026701412 scopus 로고
    • Identification of multiple active growth factors in basement membrane Matrigel suggests caution in interpretation of cellular activity related to extracellular matrix components
    • Vukicevic, S.; Kleinman, H. K.; Luyten, F. P., et al. Identification of multiple active growth factors in basement membrane Matrigel suggests caution in interpretation of cellular activity related to extracellular matrix components. Exp. Cell Res. 202:1-8; 1992.
    • (1992) Exp. Cell Res. , vol.202 , pp. 1-8
    • Vukicevic, S.1    Kleinman, H.K.2    Luyten, F.P.3


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