Isolation and culture of rat sensory neurons having distinct sensory modalities

Journal of Neuroscience Methods

Volumn 77, Issue 2, 1997, Pages 183-190

  Eckert, Stephani P ^a   Taddese, Abraha ^a,b   McCleskey, Edwin W ^a  

^a OREGON HEALTH AND SCIENCE UNIVERSITY   (United States)

^b HARVARD MEDICAL SCHOOL   (United States)

Author keywords

Mesencephalic nucleus; Nociception; Pain; Proprioception; Sensory neurobiology; Sensory neurons; Tissue culture; Tooth pulp; Trigeminal ganglia

Indexed keywords

ARTICLE; HUMAN; HUMAN TISSUE; MASSETER MUSCLE; NOCICEPTION; PAIN RECEPTOR; PRIORITY JOURNAL; RECEPTOR DENSITY; SENSORY NERVE; TOOTH PAIN; TOOTH PULP; TRIGEMINAL NERVE;

AFFINITY LABELS; ANIMALS; CARBOCYANINES; CELL CULTURE TECHNIQUES; CELL SEPARATION; CELLS, CULTURED; CENTRIFUGATION, DENSITY GRADIENT; DISSECTION; FLUORESCENT DYES; INJECTIONS, INTRAMUSCULAR; MASSETER MUSCLE; MESENCEPHALON; NEUROGLIA; NEURONS, AFFERENT; RATS; RATS, SPRAGUE-DAWLEY; SOLUTIONS; SURFACE PROPERTIES; TOOTH GERM; TRIGEMINAL GANGLION;

EID: 0031416555     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0165-0270(97)00125-8     Document Type: Article

References (17)

1. Ahlquist ML, Edwall LGA, Franzen OG, Haegerstam GAT. Perception of pulpal pain as a function of intradental nerve activity. Pain 1984;19:353-66.
2. Anderson DJ, Matthews B. Osmotic stimulation of human dentine and the distribution of dental pain thresholds. Arch Oral Biol 1967;12:417-26.
3. Baccaglini PI, Hogan PG. Some rat sensory neurons in culture express characteristics of differentiated pain sensory cells. Proc Natl Acad Sci USA 1983;80:594-8.
4. Banker G, Goslin K, editors. Culturing Nerve Cells. Cambridge, MA: The MIT Press, 1991:69-70, 229-230.
5. Cody FWJ, Lee RWH, Taylor A. A functional analysis of the components of the mesencephalic nucleus of the fifth nerve in the cat. J Physiol 1972;226:249-61.
6. Cook SP, Vulchanova L, Hargreaves KM, Elde R, McCleskey EW. Distinct ATP receptors on pain-sensing and stretch-sensing neurons. Nature 1997;387:505-8.
7. Corbin KB, Harrison F. Function of mesencephalic root of fifth cranial nerve. J Neurophysiol 1940;3:423-35.
8. Edwall L, Olgart L. A new technique for recording of intradental sensory nerve activity in man. Pain 1977;3:121-5.
9. Harsh C, Archibald SJ, Madison RD. Double-labeling of saphenous nerve neuron pools: a model for determining the accuracy of axon regeneration at the single neuron level. J Neurosci Methods 1991;39:123-9.
10. Honig MG, Hume RI. Fluorescent carbocyanine drugs allow living neurons of identified origin to be studied in long term cultures. J Cell Biol 1986;103(1):171-87.
11. Marfurt CF, Turner DF. The central projections of tooth pulp afferent neurons in the rat as determined by the transganglionic transport of horseradish peroxidase. J Comp Neurol 1984;223:535-47.
12. Narhi M, Yamamoto H, Ngassapa D, Hirvonen T. The neurophysiological basis and the role of inflammatory reactions in dentine hypersensitivity. Arch Oral Biol 1994;39:23S-30S.
13. Paxinos G, Watson, C. The Rat Brain in Stereotaxic Coordinates. New York: Academic Press, 1982.
14. 2+ currents by a μ-opioid in a defined subset of rat sensory neurons. J Neurosci 1993;13(2):867-73.
15. Sugaya A, Chudler EH, Byers MR. Uptake of exogenous fluorescent Di-I by intact junctional epithelium of adult rats allows retrograde labeling of trigeminal sensory neurons. Brain Res 1994;653:330-4.
16. Sugimoto T, Takemura M, Wakisaka S. Cell size analysis of primary neurons innervating the cornea and tooth pulp of the rat. Pain 1988;32:375-81.
17. Taddese A, Nah SY, McCleskey EW. Selective inhibition of small nociceptors. Science 1995;270:1366-9.