Hepatocyte primary culture bioassay: A simplified tool to assess the initiation of the liver regeneration cascade

Journal of Pharmacological and Toxicological Methods

Volumn 38, Issue 3, 1997, Pages 141-150

  Wang, H Helen ^a   Lautt, W Wayne ^a  

^a UNIVERSITY OF MANITOBA   (Canada)

Author keywords

Cell number; Partial hepatectomy; Plasma; Proliferative factors; Serum

Indexed keywords

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BIOASSAY; CELL COUNT; CELL CULTURE; CELL PROLIFERATION; CELL SUSPENSION; CONTROLLED STUDY; DNA SYNTHESIS; IN VITRO STUDY; LIVER CELL; LIVER REGENERATION; MALE; NONHUMAN; PARTIAL HEPATECTOMY; QUANTITATIVE ASSAY; RAT;

ANIMALS; CELL COUNT; CELL DIVISION; CELL SURVIVAL; CELLS, CULTURED; HEPATECTOMY; LIVER; LIVER REGENERATION; MALE; RATS; RATS, SPRAGUE-DAWLEY;

EID: 0031416424     PISSN: 10568719     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1056-8719(97)00079-8     Document Type: Article

References (30)

1. Alison M, Chaudry Z, Baker I, Lauder I, Pringle H (1994) Liver regeneration: A comparison of in situ hybridization for histone mRNA with bromodeoxyuridine labeling; for the detection of S-phase cells. J Histochem Cytochem 42:1603-1608.
2. Bucher NLR (1963) Regeneration of mammalian liver. Int Rev Cytol 15:245-300.
3. Counts WB, Flanim WG (1966) An artifact associated with the incorporation of thymidine into DNA preparations. Biochim Biophys Acta 114:628-630.
4. Cruveilhier J, quoted by Molne LS (1909) The: histology of liver tissue regeneration. J Path Bact 13:127-160.
5. Diehl AM, Rai RM (1996) Regulation of signal transduction during liver regeneration. FASEB J 10:215-227.
6. Fausto N, Laird AD, Webber EM (1995) Role of growth factors and cytokines in hepatic regeneration. FASEB J 9:1527-1536.
7. Fishback FC (1929) A morphologic study of regeneration of the liver after partial removal. Arch Path 7:955-977.
8. Francavilla A, Ove P, Polimeno L, Sciascia C, Coetzee ML, Stargl TE (1986) Epidermal growth factor and proliferation in rat hepatocytes in primary culture isolated at different times after partial hepatectomy. Cancer Res 46:1318-1323.
9. Gerlyng P, Stokke T, Huitfeldt HS, Stenersen T, Danielsen HE, Grotmol T, Seglen PO (1992) Analytical methods for the study of liver cell proliferation. Cytometry 13:404-415.
10. Gratzner HG (1982) Monoclonal antibody to 5-bromo-and 5-iododeoxyuridine: A new reagent for detection of DNA replication. Science 218:474-475.
11. Grisham JW (1960) Inhibitory effect of tritiated thymidine on regeneration of the liver in the young rat. proc Soc Exp Biol Med 105:555-558.
12. Hasegawa K, Watanabe K, Koga M (1982) Induction of mitosis in primary cultures of adult rat hepatocytes under serum-free conditions. Biochem Biophys Res Commun 104: 259-265.
13. Higgins GM, Anderson RM (1931) Experimental pathology of the liver. I. Restoration of the liver of the white rat following partial surgical removal. Arch Pathol 12:186-202.
14. Jezequel AM, Paulucci F, Benedetti A, Mancini R, Orlandi F (1991) Enumeration of S-phase cells in normal rat liver by immuno histochemistry using bromodeoxyuridine-antibromodeoxyuridine system. Dig Dis Sci 36:482-484.
15. Kiso S, Kawata S, Tamura S, Higashiyama S, Ito N, Tsushima H, Taniguchi N, Matsuzawa Y (1995) Role of heparin-binding epidermal growth factor-like growth factor as a hepatotrophic factor in rat liver regeneration after partial hepactectomy. Hepatology 22: 1584-1590.
16. Lautt WW, Dwan PO, Singh RR (1982) Control of the hyperglycemic response to hemorrhage in cats. Can J Physiol Pharmacol 60:1618-1623.
17. Leffert HL, Moran T, Boorstein R, Koch KS (1977) Procarcinogen activation and hormonal control of cell proliferation in differentiated primary adult rat liver cell cultures. Nature 267:58-61.
18. Leffert HL, Koch KS, Moran T, Williams M (1979) Liver cells. Methods Enzymol LVIII:536-544.
19. Lindroos PM, Zarnegar R, Michalopoulos GK (1991) Hepatocyte growth factor (Hepatopoietin A) rapidly increases in plasma before DNA synthesis and liver regeneration stimulated by partial hepatectomy and carbon tetrachloride administration. Hepatology 13: 743-749.
20. Masaki T, Tokuda M, Fujimura T, Ohnishi M, Tai Y, Miyamoto K, Itano T, Matsui H, Watanabe S, Sogawa KI, Yamada T, Konishi R, Nishioka M, Hatase O (1994) Involvement of annexin I and annexin II in hepatocyte proliferation: can annexins I and II be markers for proliferative hepatocytes? Hepatology 20(2):425-435.
21. Mathews MB, Bernstein RM, Franza Jr BR,Garrels Garrels JI (1984) Identity of the proliferating cell nuclear antigen and cyclin. Nature 309:374-376.
22. McGowan JA, Strain AJ, Bucher NLR (1981) DNA synthesis in primary cultures of adult rat hepatocytes in a defined medium: Effects of epidermal growth factor, insulin, glucagon, and cyclicAMP. J Cell Physiol 108:353-363.
23. Michalopoulos G, Cianciulli HD, Novotny AR, Kligerman AD, Strom SC, Jirtle RL (1982) Liver regeneration studies with rat hepatocytes in primary culture. Cancer Res 42:4673-4682.
24. Miyachi K, Fritzler MJ, Tan EM (1978) Autoantibody to a nuclear antigen in proliferating cells. J Immunol 121:2228-2234.
25. 3H-thymidine for measurement of DNA synthesis in rat liver: A warning. Anal Biochem 45:298-305.
26. Patterson Jr MK (1979) Measurement of growth and viability of cells in culture. Methods Enzymol LVIII:141-152.
27. Seglen PO (1976) Preparation of isolated rat liver cells. Methods Cell Biol 13:29-83.
28. Shiratori Y, Hougo S, Hikiba Y, Ohmura K, Nagura T, Okano K, Kamii K, Tanake T, Komatsu Y, Ochiai T, Tsubouchi H, Omata M (1996) Role of macrophages in regeneration of liver. Dig Dis Sci 41:1939-1946.
29. Theocharis SE, Skopelitou AS, Margeli AP, Pavlaki KJ, Kittas C (1994) Proliferating cell nuclear antigen (PCNA) expression in regenerating rat liver after partial hepatectomy. Dig Dis Sci 39:245-252.
30. Webber EM, Godowski PJ, Fausto N (1994) In vivo response of hepatocytes to growth factors requires an initial priming stimulus. Hepatology 14:489-497.