Characterization of isolated duodenal epithelial cells along a crypt- villus axis in rats fed diets with different iron content

Journal of Gastroenterology and Hepatology (Australia)

Volumn 12, Issue 12, 1997, Pages 829-838

  Oates, Phillip S ^a   Thomas, Carla ^a   Morgan, Evan H ^a  

^a UNIVERSITY OF WESTERN AUSTRALIA   (Australia)

Author keywords

Activity; Crypt villus axis; Enterocytes; Isolation; Rat; Transferrin receptor

Indexed keywords

CELL DNA;

ANIMAL CELL; ARTICLE; CELL ISOLATION; CELL VIABILITY; DNA CONTENT; DNA DETERMINATION; DNA SYNTHESIS; DUODENUM MUCOSA; EPITHELIUM CELL; INTESTINE CELL; INTESTINE CRYPT; INTESTINE VILLUS; IRON ABSORPTION; IRON INTAKE; MALE; NONHUMAN; PRIORITY JOURNAL; RAT;

EID: 0031415538     PISSN: 08159319     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1440-1746.1997.tb00380.x     Document Type: Article

References (29)

1. Potten CS, Loeffler M. Stem cells: attributes, cycles, spirals, pitfalls and uncertainties. Lessons for and from the crypt. Development 1990; 110: 1101-20.
2. Cheng H, Leblond CP. Origin, differentiation and renewal of the four main epithelial cell types in the mouse small intestine I. Columnar cell. Am. J. Anat. 1974; 141: 461-79.
3. Hermiston ML, Simon TC, Crossman MW, Gordon JI. Model systems for studying cell fate specification and differentiation in the gut epithelium: From worms to flies to mice. In: Johnson LR, ed. Physiology of the gastrointestinal tract, Vol. 1, 3rd edn. New York: Raven Press, 1994; 521-70.
4. Gavrieli Y, Sherman Y, Ben-Sasson SA. Identification of programmed cell death in situ via specific labeling of nuclear fragmentation. J. Cell Biol. 1992; 119: 493-501.
5. Potten CS, Allen TD. Ultrastructure of cell loss in intestinal mucosa. J. Ultrastruct. Res. 1977; 60: 272-7.
6. Oates PS, Morgan EH. Effects of dietary iron loading with carbonyl iron, and iron depletion, on intestinal growth, morphology and expression of transferrin receptor in the rat. Anat. Rec. 1996; 246: 364-71.
7. Bomford AB, Munro HN. Transferrin and its receptor: Their roles in cell function. Hepatology 1985; 5: 870-5.
8. Oates PS, Morgan EH. Defective iron uptake by the duodenum of Belgrade rats fed diets of different iron contents. Am. J. Physiol. 1996; 270: 826-32.
9. Stern BK. Some biochemical properties of suspensions of intestinal epithelial cells. Gastroenterology 1966; 51: 855-07.
10. Weiser MM. Intestinal epithelial cell surface membrane glycoprotein synthesis. I. An indicator of cellular differentiation. J. Biol. Chem. 1973; 248: 2536-41.
11. Bieri JG, Stoewsand GS, Briggs GM, Phillips RW, Woodard JC, Knapka JJ. Report of the American Institute of Nutrition Ad Hoc committee on standards for nutritional studies. J. Nutrition 1977; 107: 1340-8.
12. Topham RW, Eads CE, Butler BF. Alterations in the mucosal processing of iron in response to very-short-term dietary iron depletion and repletion. Biochem. J. 1992; 284: 877-84.
13. Jensen RT, Moody T, Pert C, Rivier JE, Gardner JD. Interaction of bombesin and litorin with specific membrane receptors on pancreatic acinar cells. Proc. Natl Acad. Sci. USA 1978; 75: 6139-43.
14. Oates PS, Morgan RGH. Short-term effects of feeding raw soya flour on pancreatic cell turnover in the rat. Am. J. Physiol. 1994; 247: G667-73.
15. Burton KA. A study of the conditions and mechansism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem. J. 1956; 62: 315-23.
16. Messer M, Dahlqvist A. A one-step ultramicro method for the assay of intestinal disaccharidases. Anal. Biochem. 1965; 14: 376-92.
17. Hemmaplardh D, Morgan EH. The mechanism of iron exchange between synthetic non chelators and rabbit reticulocytes. Biochim. Biophys. Acta. 1974; 373: 84-09.
18. Iacopetta BJ, Morgan EH. The kinetics of transferrin endocytosis and iron uptake from transferrin in rabbit reticulocytes. J. Biol. Chem. 1981; 258: 9108-15.
19. Crowe A, Morgan EH. Iron and transferrin uptake by brain and cerebrospinal fluid in the rat. Brain Res. 1992; 592: 8-16.
20. Hugon J, Borgers M. Ultrastructural localisation of alkaline phosphatase activity in the absorbing cells of the duodenum of mouse. J. Histochem. Cytochem. 1966; 14: 629-40.
21. Oates PS, Thomas C, Light AM. Immunohistochemical processing of tissue which allows for embedding in Araldite, and orientation and correlation between light and electron microscopic staining. J. Microscopy 1967; 187: 68-72.
22. International Committee for Standardisation in Haematology. The measurement of total and unsaturated iron-binding capacity in serum. Br. J. Haematol. 1978; 38: 281-90.
23. Ge YC, Morgan RGH. The effect of trypsin inhibitor on the pancreas and small intestine of mice. Br. J. Nutr. 1993; 70: 333-45.
24. Eade OE, Andre-Ukena S St., Beeken WL. Comparative viabilities of rat intestinal cells prepared by mechanical, enzymatic and chelating methods. Digestion 1981; 21: 25-32.
25. Wright EM, Hirayama BA, Loo DDF, Turk E, Hager K. Intestinal sugar transport In: Johnson LR, ed. Physiology of the gastrointestinal tract, Vol. 1, 3rd edn. New York: Raven Press, 1994; 1751-71.
26. Conrad ME, Weintraub LR, Crosby WH. The role of the intestine in iron kinetics. J. Clin. Invest. 1963; 43: 963-74.
27. Cazzola M, Huebers HA, Sayers MH, MacPhail AP, Eng M, Finch CA. Transferrin saturation, plasma iron turnover, and transferrin uptake in normal humans. Blood 1985; 66: 935-9.
28. Christensen AC, Huebers H, Finch CA. Effect of transferrin saturation on iron delivery in rats. Am. J. Physiol. 1978; 235: R18-22.
29. Anderson GJ, Powell LW, Halliday JW. The endocytosis of transferrin by rat intestinal epithelial cells. Gastroenterology 1994; 106: 414-22.