Rapid isolation of genomic clones for individual members of human multigene families: Identification and localisation of UBE2L4, a novel member of a ubiquitin conjugating enzyme dispersed gene family

Cytogenetics and Cell Genetics

Volumn 79, Issue 3-4, 1997, Pages 188-192

  Ardley, H C ^a   Moynihan, T P ^a   Thompson, J ^a,b   Leek, J P ^a   Markham, A F ^a   Robinson, P A ^a,b  

^a St James's University Hospital   (Ukraine)

^b LEEDS DENTAL INSTITUTE   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

UBIQUITIN CONJUGATING ENZYME;

ALTERNATIVE RNA SPLICING; ARTICLE; BACTERIOPHAGE; CHROMOSOME 19Q; DNA LIBRARY; GENE LOCATION; HUMAN; MOLECULAR CLONING; MULTIGENE FAMILY; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL;

BACTERIOPHAGE LAMBDA; BASE SEQUENCE; CHROMOSOME MAPPING; GENOMIC LIBRARY; HUMANS; LIGASES; MOLECULAR SEQUENCE DATA; MULTIGENE FAMILY; POLYMERASE CHAIN REACTION; UBIQUITIN-CONJUGATING ENZYMES;

EID: 0031412144     PISSN: 03010171     EISSN: 1424859X     Source Type: Journal    
DOI: 10.1159/000134717     Document Type: Article

References (17)

1. Cicchanovcr A: The ubiquitin-protcasome proteolytic pathway. Cell 79:13-21 (1994).
2. Goldberg AL, Rock KL: Proteolysis, proteasomes and antigen presentation. Nature 357:375-379(1992)
3. Hochstrasser M: Ubiquitin. proteasomes. and the regulation of intracellular protein degradation. Curr Opin Cell Biol 7:215-223 (1995).
4. Jalal SM, Law ME, Christensen ER, Spurbeck JL, Dewald GW: Method for sequential staining of GTL-bandcd metaphascs with fluorescent-labelled chromosome specific paint probes. Am J mcd Genet 46:98-103(1993).
5. Jentsch S: The ubiquitin-conjugation system. A Rev Genet 26:179-207 (1992).
6. Landegcnt JE, Dewal NJI, Dirks RV, Baas F, Van der Ploeg M: Use of whole cosmid cloned genomic sequences for chromosomal localisation by non-radioactivc in situ hybridisation. Hum Genet 77:336-370(1987).
7. Lardclli M, Lendahl U: Generating bacteriophage 1 sub-libraries enriched for rare clones. BioTechni-ques 16:420-422 (1994).
8. Moynihan TP. Ardley HC, Leek JP, Thompson J. Brin-dle NS. Markham AF. Robinson PA: Characterization of a human ubiquitin-conjugating enzyme gene. UBE2L3. Mammal Genome 7:520-525 (1996).
9. Nehls M. Pfeifer D. Boehm T: Exon amplification from complete libraries of genomic DNA using a novel phage vector with automatic plasmid excision facility: application to the mouse neurofibromatosis-1 locus. Oncogene 9:2169-2175 (1994).
10. Nuber U, Schwarz S, Kaiser P, Schneider R. Scheffner M: Cloning of human ubiquitin-conjugating enzymes UbcH6 and UbcH7 (E2-F1) and characterization of their interaction with E6-AP and RSP5. J biol Chem 271:2795-2800 (1996).
11. Paolini R. Kinet J-P: Cell surface control of the multiu-biquitination and deubiquitinalion of high-aflinity immunoglobulin E receptors. EMBO J 12:779-786 (1993).
12. Pinkel D, Strawme T, Gray JW: Cytogenetic analysis using quantitative, high sensitivity fluorescence hybridisation. Proc natl Acad Sci. USA 83:2934-2939(1986)
13. Robinson PA. Leek JP, Thompson J. Carr IM. Bailey A, Moynihan TM. Coletta PL. Lench NJ, Markham AF: A human conjugating enzyme. L-UBC. maps in the Alzheimer’s disease locus on chromosome 14q24.3. Mammal Genome 6:725-731 (1995).
14. Sambrook J, Fritsch F, Maniatis T: Molecular Cloning: A Laboratory Manual. 2nd Ed (Cold Spring Harbor Laboratory Press. Cold Spring Harbor 1989).
15. Scheffner M. Huibregtse JM. Howley PM: Identification of a human ubiquitin-conjugating enzyme that mediates the E6-AP-dependcnt ubiquitination of p53. Proc natl Acad Sci, USA 91:8797-8801 (1994).
16. Seufert W. Jentsch S: Ubiquitin conjugating enzymes UBC-4 and UBC5 mediate selective degradation of short-lived and abnormal proteins. EMBO J 9:543-550(1990).
17. Yost HJ. Petersen RB. Lindquist S: Posttranscriptional regulation of heal shock protein synthesis in Drosophila. in Morimoto Rl. Tissicres A. Gcorgopou-los C (eds): Stress Proteins in Biology and Medicine, pp 379-409 (Cold Spring Harbor Laboratory Press. Cold Spring Harbor 1990).