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note
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The present GC-MS method for PQQ does not absolutely require a stable isotopic internal standard (IS); any acid compound with a molecular weight of around 300, which is resistant to heat decomposition, can be used as an IS, if its retention time is close to that of PQQ. The SIM without any IS can also be used for semiquantitation of PQQ, although the sensitivity may be much lower.
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17
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85030334818
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note
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The use of lower concentrations of PTMA hydroxide (e.g., 0.1 M PTMA hydroxide in methanol) results in poor reaction.
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18
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85030333036
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note
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When heating the vials at 100°, leakage of the vapor in the vials may occur, and this should be avoided. To maintain tight capping, the use of Teflon/rubber-laminated disks (Pierce. Rockford. IL) is useful.
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19
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0026679258
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