Rapid fluorometric assay of bacterial density in lake water and seawater

Limnology and Oceanography

Volumn 42, Issue 7, 1997, Pages 1629-1634

  Tranvik, Lars J ^a,b  

^a LUND UNIVERSITY   (Sweden)

^b LINKÖPING UNIVERSITY   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

ASSAY; BACTERIAL DENSITY; FLUOROMETRIC METHOD; FRESHWATER; SEAWATER;

ALGAE; BACTERIA (MICROORGANISMS); VISCUM ALBUM;

EID: 0031410825     PISSN: 00243590     EISSN: None     Source Type: Journal    
DOI: 10.4319/lo.1997.42.7.1629     Document Type: Article

References (23)

1. AMMERMAN, J. W., J. A. FUHRMAN, Å. HAGSTRÖM, AND F. AZAM. 1984. Bacterial growth in seawater. I. Growth kinetics and cellular characteristics in seawater cultures. Mar. Ecol. Prog. Ser. 18: 31-39.
2. AMON, R. M. W., AND R. BENNER. 1996. Bacterial utilization of different size classes of dissolved organic matter. Limnol. Oceanogr. 41: 41-51.
3. BJORNSEN, P. K. 1986. Bacterioplankton growth yield in continuous seawater cultures. Mar. Ecol. Prog. Ser. 30: 191-196.
4. BUSHAW, K. L., AND OTHERS. 1996. Photochemical release of biologically available nitrogen from aquatic dissolved organic matter. Nature 381: 404-407.
5. CARLSSOM, P., E. GRANÉLI, P. TESTER, AND L. BONI. 1995. Influences of riverine humic substances on bacteria, protozoa, phytoplankton, and copepods in a coastal plankton community. Mar. Ecol. Prog. Ser. 127: 213-221.
6. CHIN-LEO, G., AND D. L. KIRCHMAN. 1990. Unbalanced growth in natural assemblages of marine bacterioplankton. Mar. Ecol. Prog. Ser. 63: 1-8.
7. DEL GIORGIO, P. A., D. F. BIRD, Y. T. PRAIRIE, AND D. PLANAS. 1996. Flow cytometric determination of bacterial abundance in lake plankton with the green nucleic acid stain SYTO 13. Limnol. Oceanogr. 41: 783-789.
8. FELIP, M., M. L. PACE, AND J. J. COLE. 1996. Regulation of planktonic bacterial growth rates: The effects of temperature and resources. Microb. Ecol. 31: 15-28.
9. HAGSTROM, Å., J. W. AMMERMAN, S. HENRICHS, AND F. AZAM. 1984. Bacterioplankton growth in seawater. II. Organic matter utilization during steady-state growth in seawater cultures. Mar. Ecol. Prog. Ser. 18: 41-48.
10. JØRGENSEN, N. O. G., N. KROER, R. B. COFFIN, X.-H. YANG, AND C. LEE. 1993. Dissolved free amino acids, combined amino acids, and DNA as sources of carbon and nitrogen to marine bacteria. Mar. Ecol. Prog. Ser. 98: 135-148.
11. LEE, R. E. 1989. Phycology. 2nd ed. Cambridge Univ. Press.
12. Li, W. K. W., J. F. JELLETT, AND P. M. DICKIE. 1995. DNA distributions in planktonic bacteria stained with TOTO or TO-PRO. Limnol. Oceanogr. 40: 1485-1495.
13. LINDELL, M. J., W. GRANÉLI, AND L. TRANVIK. 1995. Enhanced bacterial growth in response to photochemical transformation of dissolved humic matter. Limnol. Oceanogr. 40: 195-199.
14. MARIE, D., D. VALOUT, AND F. PARTENSKY. 1996. Application of the novel nucleic acid dyes YOYO-1, YOPRO-1, and Pico-Green for flow cytometric analyses of marine prokaryotes. Appl. Environ. Microbiol. 62: 1649-1655.
15. MCCOY, W. F., AND B. H. OLSON. 1985. Fluorometric determination of the DNA concentration in municipal drinking water. Appl. Environ. Microbiol. 49: 811-817.
16. PAUL, J. H., AND D. J. CARLSON. 1984. Genetic material in the marine environments: Implications for bacterial DNA. Limnol. Oceanogr. 29: 1091-1097.
17. _, AND B. MYERS. 1982. Fluorometric determination of DNA in aquatic microorganisms by use of Hoechst 33258. Appl. Environ. Microbiol. 43: 1393-1399.
18. PORTER, K. G., AND Y. S. FEIG. 1980. The use of DAPI for identifying and counting aquatic microflora. Limnol. Oceanogr. 25: 943-948.
19. SØNDERGAARD, M., AND M. MIDDELBOE. 1995. A cross-system analysis of labile dissolved organic carbon. Mar. Ecol. Prog. Ser. 118: 283-294.
20. TRANVIK, L. J. 1988. Availability of dissolved organic carbon for planktonic bacterial in oligotrophic lakes of differing humic content. Microb. Ecol. 16: 311-322.
21. _. 1990. Bacterioplankton growth on fractions of dissolved organic carbon of different molecular weights from humic and clear waters. Appl. Environ. Microbiol. 56: 1672-1677.
22. _, AND N. O. G. JØRGENSEN. 1995. Colloidal and dissolved organic matter in lake water: Carbohydrate and amino acid composition, and ability to support bacterial growth. Biogeochemistry 30: 77-97.
23. ZWEIFEL, U.-L., B. NORRMAN, AND A. HAGSTRÖM. 1993. Consumption of dissolved organic carbon by marine bacteria and demand for inorganic nutrients. Mar. Ecol. Prog. Ser. 101: 23-32.