Molecular cloning and characterization of cDNAs coding for ferredoxin-dependent glutamate synthase from Vitis vinifera

Physiologia Plantarum

Volumn 101, Issue 1, 1997, Pages 220-228

  Loulakakis, Konstantinos A ^a   Roubelakis Angelakis, Kalliopi A ^a  

^a UNIVERSITY OF CRETE   (Greece)

Author keywords

Ammonia assimilation; cDNA cloning; Gene expression; Glutamate synthase; Grapevine; Nitrogen metabolism; Vitis vinifera

Indexed keywords

AMINO ACID SEQUENCE; AMMONIUM ASSIMILATION; CELL CULTURE; COMPLEMENTARY DNA; DNA BLOT ANALYSIS; ENZYME ACTIVITY; FERREDOXIN; GENE EXPRESSION; GLUTAMATE SYNTHASE; MOLECULAR CLONING; NITRATE; NITROGEN; NORTHERN BLOTTING;

VITIS VINIFERA;

EID: 0031398003     PISSN: 00319317     EISSN: None     Source Type: Journal    
DOI: 10.1034/j.1399-3054.1997.1010129.x     Document Type: Article

References (37)

1. Avila, C., Marquez, A. J., Pajuelo, P., Cannell, M. E., Wallsgrove, R. M. & Forde, B. G. 1993. Cloning and sequence analysis of a cDNA for barley ferredoxin-dependent glutamate synthase and molecular analysis of photorespiralory mutants deficient in the enzyme. - Planta 189: 475-483.
2. Church, G. M. & Gilbert, W. 1984. Genomic sequencing. Proc. Natl. Acad. Sci. USA 81: 1991-1995.
3. Feinberg, A. P. & Vogelstein, B. 1983. A technique for radiolabeling DNA restriction endonucluasc fragments to high specific activity. - Anal. Biochem. 132: 6-13.
4. Gregerson, R. G., Miller, S. S., Twary, S. N., Gantt, J. S. & Vance, C. P. 1993. Molecular characterization of NADH- glutamate synthase from alfalfa nodules. - Plant Cell 5: 215-226.
5. Hayakawa, T., Kamachi, K., Oikawa, M., Ojima, K. & Yamaya, T. 1990. Response of glutamine synthetase and glutamate synthase isoforms to nitrogen sources in rice cell cultures. Plant Cell Physiol. 31: 1071-1077.
6. _ , Yamuya, T., Kamachi, K. & Ojima, K. 1992. Purification, characterization, and immunological properties of NADH-depcndent glutamate synthase from rice cell cultures. - Plant Physiol. 98: 1317-1322.
7. Hecht, U., Oclmuller, R., Schmidt, S. & Mohr, H. 1988. Action of light, nitrate and ammonium on the levels of NADH- and ferredoxin-dependent glutamate synthases in the cotyledons of mustard seedlings. - Planta 175: 130-138.
8. Kendall, A. C., Wallsgrove, R. M., Hall, N. P., Turner, J. C. & Lea, P. J. 1986, Carbon and nitrogen metabolism in barley (Hardeum vulgare L.) mutants lacking f'erredoxin-dependent glutamate synthase. - Planta 168: 316-323.
9. Lea, P. J., Robinson, S. A. & Stewart, G. R. 1990. The enzymes and metabolism of glutamine, glutamate and asparagine. In The Biochemistry of Plants: A Comprehensive Treatise (B. J. Miflin and P. J. Lea. eds), Vol. 16. pp. 121-159. Academic Press, New York. NY. ISBN 0-12-675410-0.
10. _ , Blackwell, R. D. & Joy, K. W. 1992. Ammonia assimilation in higher plants. - In Nitrogen Metabolism of Plants (K. Mengel and P. J. Pilbeam, eds). Vol. 3. pp. 153-156, Clarendon Press. Oxford. ISBN 0-19-857752-4.
11. Lodhi, M. A., Ye, G.-N., Weedcn, N. F. & Reisch, B. L. 1994. A simple and efficient method for DNA extraction from grapevine cultivars and Vitis species. - Plant Mol. Biol. Rep. 12: 6-13.
12. Loulakakis, K. A. & Roubelakis-Angelakis, K. A. 1990a. Intracellular localization and properties of NADH-glutamate dehydrogenase from Vitis vinifera L.: Purification and characterization of the major leaf isoenzyme. - J. Exp. Bot. 41: 1223-1230.
13. _ & Roubelakis-Angelakis, K. A. 1990b Immunocharaeterization of NADH-glutamate dehydrogenase from Vitis vinifera L. - Plant Physiol. 94: 109-113.
14. _ & Roubelakis-Angelakis, K. A. 1991. Plant NAD(H)- glutamate dehydrogenase consists of two suhunit polypeptides and their participation in the seven isoenzymes occurs in an ordered ratio. - Plant Physiol. 97: 104-111.
15. _ & Roubelakis-Angelakis, K. A. 1992. Ammonium-induced increase in NADH-gluiamate dehydrogenase activity is caused by de novo synthesis of the a-subunit. - Planta 187: 322-327.
16. _ & Roubelakis-Angelakis, K. A. 1996a. The seven NAD(H)- glutamate dehydrogenase isoenzymes exhibit similar anabolic and catabolic activities, - Physiol. Plant. 96: 29-35.
17. _ & Roubelakis-Angelakis, K. A. 1996b, Characterization of Vitis vinifera L. glutamine synthetase and molecular cloning of cDNAs for the cytosolic enzyme. - Plant Mol. Biol. 31: 983-992.
18. _ , Roubelakis-Angelakis, K. A. & Kanellis, A. K. 1994. Regulation of glutamate dehydrogenase and glutamine synthetase in avocado fruit during development and ripening. Plant Physiol. 106: 217-222.
19. _ , Roubelakis-Angelakis, K. A. & Kanellis, A. K. 1996. Isolation of functional RNA from grapevine tissues poor in nucleic acid content, - Am. J. Enol. Vitic. 47: 181-185.
20. Lowry, O., Rosebrough, N. J., Farr, A. L. & Randall, R. J. 1951. Protein measurement with the Folin phenol reagent. - J. Biol. Chem. 193: 265-275.
21. Matoh, T. & Takahashi, E. 1982. Changes in the activities of ferredoxin- and NADH-glutamate synthase during seedling development of peas. - Planta 154: 289-294.
22. McGrath, R. B. & Coruzzi, G. M. 1991. A gene network controlling glutamine and asparagine biosynthesis in plants. Plant J. 1: 275-280.
23. Miflin, B. J. & Lea, P. J. 1980. Ammonia assimilation. - In The Biochemistry of Plants (B. J. Miflin, ed.). Vol. 5. pp. 169-202. Academic Press. New York, NY. ISBN 0-12-675405-5.
24. Murashige, T. & Skoog, F. 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures. - Physiol. Plant. 15: 473-497.
25. Nalbantoglu, B., Hirasawa, M., Moomaw, C., Nguyen, H., Knaff, D. B. & Allen, R. 1994. Cloning and sequencing of the gene encoding spinach ferredoxin-dependent glutamate synthase. - Biochim. Biophys. Acta 1183: 557-561.
26. Oaks, A. 1994. Primary nitrogen assimilation in higher plants and its regulation. - Can. J. Bot. 72: 739-750.
27. Oliver, G., Gosset, G., Sanchez-Pescador, R., Lozoya, E., Ku, L. M., Flores, N., Becerril, B., Valle, F. & Bolivar, F. 1987. Determination of the nucleotide sequence for the glutamate synthase structural genes of Eschrichia coli K-12. - Gene 60: 1-11.
28. Redinbaugh, M. G. & Campbell, W. H. 1993. Glutamine synthetase and ferredoxin-dependent glutamate synthasc expression in the maize (Zea mays) root primary response to nitrate. - Plant Physiol. 101: 1249-1255.
29. Sakakibara. H., Watanabe, M., Hase. T. & Sugiyama, T. 1991. Molecular cloning and characterization of complementary DNA encoding for ferredoxin-dependent glutamate synthase in maize leaf. - J. Biol. Chem. 266: 2028-2035.
30. _ , Kawabata, S., Takahashi, H., Hase, T. & Sugiyama, T. 1992. Molecular cloning of the family of glutamine synthetase genes from maize: Expression of genes for glutamine synthctase and ferredoxin-dependent glutamate synthase in photosynthetic and non-photosynthetic tissues. Plant Cell Physiol. 33: 49-58.
31. Sambrook, J., Fritsch, E. F. & Maniatis, T. 1989. Molecular Cloning. A Laboratory Manual. 2nd Ed. - Cold Spring Harbor, Laboratory Press. Cold Spring Harbor. NY. ISBN 0-87969-309-6.
32. Sanger, F., Nicklen, S. & Coulson, A. R. 1977. DNA sequencing with chain-terminating inhibitors. - Proc. Natl. Acad. Sci. USA 74: 5463-5467.
33. Somerville, C. R. & Ogren, W. L. 1980. Inhibition of photosynthesis in Arabidopm mutants lacking leaf glutamate synthase activity. - Nature 286: 257-259.
34. Suzuki, A. & Gadal, P. 1984. Glutamate synthase: Physicochemical and functional properties of different isoforms in higher plants and in other organisms. - Physiol. Veg. 22: 471-486.
35. _ , Vidal, J. & Gadal, P. 1982. Glutamate synthase isoforms in rice. Immunological studies of enzymes in green leaf, etiolated leaf, and root tissues. - Plant Physiol. 70: 827-832.
36. Syntiehuki, K. M., Loulakakis, K. A. & Roubelakis-Angelakis, K. A. 1996. The amino acid sequence similarity of plant glutumnle dehydrogenuse with the extremophilic archaeal enzyme conforms to its stress related function. - Gene 168: 87-92.
37. Zehnacker, C., Becker, T. W., Suzuki, A., Currayol, F., Caboche, M. & Hirel, B. 1992. Purification and properties of tobacco ferredoxin-dependent glutamate synthase. and isolation of corresponding cDNA clones, Light-inducibility and or- gan-specificity of gene transcription. - Planta 187: 266-274.