mRNA and protein expression of nitric oxide synthase and adrenoceptor alpha 1 in young and old rat penile tissues

British Journal of Urology

Volumn 80, Issue 2, 1997, Pages 300-306

  Dahiya, R ^a   Lin, A ^a   Bakircioglu, M E ^a   Huang, S T ^a   Lue, T F ^a  

^a VETERANS AFFAIRS MEDICAL CENTER   (United States)

Author keywords

Adrenergic staining; Adrenoceptor; Gene expression; NADPH diaphorase staining; Nitric oxide synthase; Pelvic ganglion; RT PCR

Indexed keywords

ALPHA 1 ADRENERGIC RECEPTOR; BETA ACTIN; FLUORESCENT DYE; LIPOFUSCIN; MESSENGER RNA; NEUROTRANSMITTER; NITRIC OXIDE SYNTHASE; OLIGONUCLEOTIDE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE DEHYDROGENASE;

ADRENERGIC NERVE; AGE; ANIMAL TISSUE; ARTICLE; CELL STRUCTURE; CONTROLLED STUDY; CORPUS CAVERNOSUM; GANGLION; GENE EXPRESSION; IMPOTENCE; MALE; MESSENGER RNA SYNTHESIS; NERVE CELL; NONHUMAN; PELVIS; PENIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; RAT; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SMOOTH MUSCLE TONE; STAINING;

EID: 0031397529     PISSN: 00071331     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1464-410x.1997.00361.x     Document Type: Article

References (26)

1. Kaiser FE. Sexuality and impotence in the aging man. Clin Geriatr Med 1991: 7: 63-72
2. Saenz de Tejada I, Kim N, Lagan I, Krane R), Goldstein I. Regulation of adrenergic activity in penile corpus cavernosum. J Vrol 1989; 142: 1117-21
3. Kirkeby HJ, Forman A. Sorensen S. Andersson K-E. Alphaadrenoceptor function in isolated penile circumflex veins from potent and impotent men. J Vrol 1989: 142: 1 369-71
4. Bylund DB. Subtypes of al and ot2-adrenergic receptors. FASER J 1992; 6: 832-9
5. Molderings GJ, Gothert M. Ahlen HV, Porst H. Noradrenaline release in human corpus cavernosum and its modulation via presynaptic a2 adrenoceptors. Fundam Clin Pharmacol 1989; 3: 497-504
6. Burnett AL. Lowenstein CJ, Bredt DS, Chang TSK. Synder SH. Nitric oxide: a physiologic mediator of penile erection. Science 1992; 257: 401-3
7. Kim N, Vardi Y, Padma-Nathan H. Daley J. Goldstein I. Saenz de Tejada I. Oxygen tension regulates the nitric oxide pathway, physiologic role in penile erection. J Clin Invest 1993: 91: 437-42
8. Ignarro LJ. Bush PA, Buga GM, Wood KS. Fukuto JM. Rajfer J. Nitric oxide and cyclic GMP formation upon electrical stimulation cause relaxation of corpus cavernosum smooth muscle. Riochem Biophys Res Commun 1990: 170: 843-50
9. Christ GJ, Maayani S, Valcic M. Melman A. Pharmacologie studies of human erectile tissue: characteristics of spontaneous contraction and alterations in alpha-adrenoceptor responsiveness with age and disease in isolated tissues. Rr } Pharmacol 1990; 101: 375-81
10. Christ GJ, Stone BS, Melman A. Age-dependent alterations in the efficacy of phenylephrine-induced contractions in vascular smooth muscle isolated from the corpus cav-ernosum of impotent men. Can J Physiol Pharmacol 1991: 69: 909-13
11. Akkus EA, Carrier S. Baba K et al. Structural alterations in the tunica albuginea of the penis: impact of Peyronie's disease, ageing and impotence. Br }Urol 1997; 79: 47-53
12. Mersdorf A. Goldsmith PC, Diederichs W et al. Ultrastructural changes in impotent penile tissue: a comparison of 65 patients. J Urol 1991: 145: 749-58
13. de la Torre JC, Surgeon JW. A methodological approach to rapid and sensitive monoamine histofluorecence using a modified glyoxylic acid technique: the SPG method. Histochemistry 1976: 49: 81-93
14. Alm P, Larsson B, Ekblad E, Sunder F. Andersson KE. Immunohistochemical localization of peripheral nitric oxide synthase containing nerves using antibodies raised against synthesized C- and N- terminal fragments of a cloned enzyme from rat brain. Acta Physiol Scand 1993: 148: 421-9
15. Dahiya R, Deng G, Chen K, Haughney PC. Cunha GR, Narayan P. New approach to hot-start polymerase chain reaction using Taq DNA polymerase antibody. Urol Oncol 1995: 1: 42-6
16. Dahiya R. Chen K-M, Chui RM, Haughney PC, Narayan P. p53 tumor-suppressor gene mutations are mainly localized on exon 7 in human primary and metastatic prostate cancer. Br J Cancer 1996: 74: 264-8
17. Dahiya R, Park H-D, Cusick J, Vessella RL, Fournier G, Narayan P. Inhibition of tumorigenic potential and prostatespecific antigen expression in LNCap human prostate cancer cell line by 13-cis-retinoic acid. Inter J Cancer 1994; 59: 126-32
18. Dahiya R. DNA repair genes in human fetal and adult prostatic tissues and cancer cell lines using differential RT-PCR. Urol Oncol 1996; 2: 52-6
19. Dahiya R. Lee C, Haughney PC, Chui R, Ho R, Deng G. Differential gene expression of TGFa. TGF. EGF, KGF and their receptors in fetal and adult prostate and cancer cell lines. Urology 1996; 48: 963-70
20. Garban H, Vernet D, Freedman A. Rajfer J. Cadavid NG. Effect of aging on nitric oxide-mediated penile erection in rats. Am J Physiol 1995; 268: 467-75
21. Carrier S. Zvara P, Nunes L, Wee KM, Rehman J. Lue TF. Regeneration of nitric oxide synthase-containing nerves after cavernous nerve neurotomy in the rat. J Ural 1995: 153: 1722-7
22. Dail WG Jr, Evan AP Eason HR. The major ganglion in the pelvic plexus of the male rat. Cell Tissue Res 1975; 159: 49-62
23. Vincent SR, Hope BT. Neurons that say NO. Trends Neurosci 1992: 15: 108-13
24. Adams MA, Banting J, Manabe K, Morales A, Heaton J. The major role for nitric oxide in the penis is to regulate the vasoconstrictor actions of endothelins. J Urol (in press)
25. Partanen M, Sanier RM, Hervonen A. The effect of aging on the histochemically demonstrable catecholamines in the hypogastric (main pelvic) ganglion. Eislöchern j 1980; 12: 527-35
26. Hervonen A, Vaalasti A, Partanen M, Kanerva L. Hervonen H. Effects of ageing on the histochemically demonstrable catecholamines and acetylcholinesterase of human sympathetic ganglia. J Neurocytol 1978; 7: 11-5