Preparation and purification of antibodies specific to human neuronal voltage-dependent calcium channel subunits

Brain Research Protocols

Volumn 1, Issue 3, 1997, Pages 307-319

  Beattie, Ruth E ^a   Volsen, Stephen G ^a   Smith, Dawn ^a   McCormack, Alison L ^a   Gillard, Samantha E ^a   Burnett, J Paul ^a   Ellis, Steven B ^b   Gillespie, Alison ^b   Harpold, Michael M ^b   Smith, William ^a  

^a ELI LILLY AND COMPANY   (United States)

^b SIBIA Corp   (United States)

Author keywords

Antibody to fusion protein; Calcium channel; Characterisation of specific antibody; Fusion protein; Purification of specific antibody

Indexed keywords

ANTIBODY; ANTIGEN; CALCIUM CHANNEL; GLUTATHIONE TRANSFERASE; HYBRID PROTEIN; POLYCLONAL ANTIBODY;

AMINO ACID SEQUENCE; ANTIBODY PRODUCTION; ANTIBODY SPECIFICITY; ARTICLE; BRAIN; CONTROLLED STUDY; DNA SEQUENCE; ENZYME LINKED IMMUNOSORBENT ASSAY; HUMAN; HUMAN CELL; IMMUNOBLOTTING; IMMUNOFLUORESCENCE; NONHUMAN; PRIORITY JOURNAL; PURIFICATION; RABBIT;

ANIMALS; ANTIBODIES; ANTIBODY SPECIFICITY; ANTIGENS; BLOTTING, WESTERN; CALCIUM CHANNELS; CELL LINE; ELECTROPHYSIOLOGY; ENZYME-LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; FLUORESCENT ANTIBODY TECHNIQUE; HUMANS; IMMUNIZATION; NEURONS; RABBITS; RECOMBINANT FUSION PROTEINS;

EID: 0031396782     PISSN: 1385299X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1385-299X(97)00004-4     Document Type: Article

References (18)

1. Bleakman, D., Bowman, D., Bath, C.P., Brust, P.F., Johnson, E.C., Deal, C.R., Miller, R.J., Ellis, S.B., Harpold, M.M., Hans, M. and Grantham, C.J., Characteristics of a human N-type calcium channel expressed in HEK293 cells, Neuropharmacology, 34 (1995) 753-765.
2. Brust, P.F., Simerson, S., McCue, A.F., Deal, C.R., Schoonaker, S., Williams, M.E., Velicelibi, G., Johnson, E.C., Harpold, M.M. and Ellis, S.B., Human neuronal voltage-dependent calcium channels: studies on subunit structure and role in channel assembly, Neuropharmacology, 32 (1993) 1089-1102.
3. Catterall, W.A., Structure and function of voltage-gated ion channels, Trends Neurosci., 16 (1993) 500-506.
4. Corvalan, J.R.F. and Smith, W., Construction and characterisation of a hybrid-hybrid monoclonal antibody recognising both carcinoembryonic antigen (CEA) and vinca alkaloids, Cancer Immunol. Immunother., 24 (1987) 127-132.
5. Day, N.C., Shaw, P.J., McCormack, A.L., Craig, P.J., Smith, W., Beattie, R., Williams, T.L., Ellis, S.B., Ince, P.G., Harpold, M.M., Lodge, D. and Volsen, S.G., Distribution of alpha (1A), alpha (1B) and alpha (1E) voltage dependent calcium channel subunits in the human hippocampus and parahippocampal gyrus, Neuroscience, 71 (1996) 1013-1024.
6. Harlow, E. and Lane, D. (Eds.), Antibodies: a Laboratory Manual, Cold Spring Harbor Laboratories, Cold Spring Harbor, NY, 1988.
7. HMSO, Guidance on the Operation of the Animals (Scientific Procedures), HMSO, London, 1986.
8. 2+ channels, J. Biol. Chem., 267 (1992) 1403-1406.
9. PhastSystem™ Owners Manual, Pharmacia Biotech.
10. Smith, W., Jarrett, A.L., Beattie, R.E. and Corvalan, J.R.F., Immunoglobulins secreted by a hybrid-hybridoma: analysis of chain assemblies, Hybridoma, 11 (1992) 87-98.
11. 2+ channels, Trends Pharmacol. Sci., 12 (1991) 349-354.
12. Volsen, S.G., Day, N.C., McCormack, A.L., Smith, W., Craig, P.J., Beattie, R., Ince, P.G., Shaw, P.J., Ellis, S.B., Gillespie, A., Harpold, M.M. and Lodge, D., The expression of neuronal voltage dependent calcium channels in human cerebellum, Mol. Brain Res., 34 (1995) 271-282.
13. Volsen, S.G., Day, N.C., McCormack, A.L., Smith, W., Craig, P.J., Beattie, R.E., Smith, D., Ince, P.G., Shaw, P.J., Ellis, S.B., Mayne, N., Burnett, P.J., Gillespie, A. and Harpold, M.M., The expression of voltage-dependent calcium channel beta subunits in human cerebellum, J. Neurosci., submitted.
14. Williams, M.E., Brust, P.F., Feldman, D.H., Patthi, S., Simerson, S., Maroufi, A., McCue, A.F., Velicelebi, G., Ellis, S.B. and Harpold, M.M., Structure and functional expression of an omega-conotoxin-sensitive human N-type calcium channel, Science, 257 (1992) 389-395.
15. Williams, M.E., Feldman, D.H., McCue, A.F., Brenner, R., Velicelebi, G., Ellis, S.B. and Harpold, M.M., Structure and functional expression of alpha1, alpha2, and beta subunits of a novel human neuronal calcium channel subtype, Neuron, 8 (1992) 71-84.
16. 1E calcium channel subtypes, J. Biol. Chem., 269 (194) 22347-22357.
17. 1A sequence, Manuscript in preparation.
18. 4 sequence, Manuscript in preparation.