Site-Directed mutagenesis of his-176 and glu-177 in pseudomonas aeruginosa alkaline protease: Effect on catalytic activity

Journal of Fermentation and Bioengineering

Volumn 84, Issue 6, 1997, Pages 588-590

  Miyajima, Yoshiro ^a   Fukushima, Jun ^b   Kawamoto, Susumu ^b   Okuda, Kenji ^b   Shibano, Yuji ^c   Hata, Yasuo ^d   Morihara, Kazuyuki ^a  

^a UNIVERSITY OF EAST ASIA   (Japan)

^b YOKOHAMA CITY UNIVERSITY   (Japan)

^c BIOMOL ENG RESEARCH INSTITUTE   (Japan)

^d KYOTO UNIVERSITY   (Japan)

Author keywords

Active site; Alkaline protease; Pseudomonas aeruginosa; Site directed mutagenesis

Indexed keywords

AMINO ACIDS; BIOCONVERSION; CATALYST ACTIVITY; COLIFORM BACTERIA; ENZYMES; MOLECULAR STRUCTURE; MUTAGENS;

ALKALINE PROTEASE; BIOTRANSFORMATION; GLUTAMINE; PROTEOLYTIC ACTIVITY;

MUTAGENESIS;

EID: 0031337397     PISSN: 0922338X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0922-338X(97)81916-2     Document Type: Article

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