Biochemical analysis of the response in rat bone marrow cell cultures to mechanical stimulation

Bio-Medical Materials and Engineering

Volumn 7, Issue 6, 1997, Pages 369-377

  Yoshikawa, Takafumi ^a   Peel, Sean A F ^b   Gladstone, Janice R ^b   Davies, John E ^b  

^a NARA MEDICAL UNIVERSITY   (Japan)

^b UNIVERSITY OF TORONTO   (Canada)

Author keywords

alkaline phosphatase; mechanical stimulation; osteocalcin; Osteogenesis; rat marrow cells

Indexed keywords

ALKALINITY; BONE; CELL CULTURE; PHOSPHATASES; RATS;

ALKALINE PHOSPHATASE; ALKALINE PHOSPHATASE ACTIVITY; BIOCHEMICAL ANALYSIS; BONE FORMATION; DRY WEIGHT; MECHANICAL STIMULATION; OSTEOCALCIN; OSTEOGENESIS; RAT BONE MARROW CELLS; RAT MARROW CELL;

CELLS;

ALKALINE PHOSPHATASE; DEXAMETHASONE; DNA; GLUCOCORTICOID; OSTEOCALCIN; TRYPSIN;

ANIMAL; ARTICLE; BONE DEVELOPMENT; BONE MARROW CELL; CATTLE; CELL CULTURE; CELL DIFFERENTIATION; CULTURE MEDIUM; CYTOLOGY; DRUG EFFECT; FEMUR; FETUS BLOOD; MALE; MECHANICAL STRESS; PHYSIOLOGY; RAT; REPRODUCIBILITY; STIMULATION; TIME; WISTAR RAT;

ALKALINE PHOSPHATASE; ANIMALS; BONE MARROW CELLS; CATTLE; CELL DIFFERENTIATION; CELLS, CULTURED; CULTURE MEDIA; DEXAMETHASONE; DNA; FEMUR; FETAL BLOOD; GLUCOCORTICOIDS; MALE; OSTEOCALCIN; OSTEOGENESIS; PHYSICAL STIMULATION; RATS; RATS, WISTAR; REPRODUCIBILITY OF RESULTS; STRESS, MECHANICAL; TIME FACTORS; TRYPSIN;

EID: 0031317585     PISSN: 09592989     EISSN: 18783619     Source Type: Journal    
DOI: None     Document Type: Article

References (26)

1. C.H. Turner, M.R. Forwood, J.Y. Rho and T. Yoshikawa, Mechanical loading thresholds for lamellar and woven bone formation, J. Bone Miner. Res. 9 (1994), 87–97.
2. R.A. Dodds, N. Ali, M.J. Pead and L.E. Lanyon, Early loading-related changes in the activity of glucose 6-phosphate dehydrogenase and alkaline phosphatase in osteocytes and periosteal osteoblasts in rat fibulae in vivo, J. Bone Miner. Res. 8 (1993), 261–267.
3. T.M. Skerry, R. Suswillo, A.J. el Haj, N.N. Ali, R.A. Dodds and L.E. Lanyon, Load-induced proteoglycan orientation in bone tissue in vivo and in vitro, Calcif. Tissue Int. 46 (1990), 318–326.
4. G.A. Rodan, T. Mensi and A. Harvey, A quantitative method for the application of compressive forces to bone in tissue culture, Calcif. Tissue Int. 18 (1975), 125–131.
5. T. Kubota, M. Yamauchi, J. Onozaki, S. Sato, Y. Suzuki and J. Sodek, Influence of an intermittent compressive force on matrix protein expression by ROS 17/2.8 cells, with selective stimulation of osteopontin, Arch. Oral Biol. 38 (1993), 23–30.
6. I. Binderman, U. Zor, A.M. Kaye, Z. Shimshoni, A. Harell and D. Somjen, The transduction of mechanical force into biochemical events in bone cells may involve activation of phospholipase A2, Calcif. Tissue Int. 42 (1988), 261–266.
7. S. Hasegawa, S. Sato, S. Saito, Y. Suzuki and D.M. Brunette, Mechanical stretching increases the number of cultured bone cells synthesizing DNA and alters their pattern of protein synthesis, Calcif. Tissue Int. 37 (1985), 431–436.
8. C. Maniatopoulos, J. Sodek and A.H. Melcher, Bone formation in vitro by stromal cells obtained from bone marrow of young adult rats, Cell Tissue Res. 254 (1988), 317–330.
9. J.E. Davies, R. Chernecky, B. Lowenberg and A. Shiga, Deposition and resorption of calcified matrix in vitro by rat marrow cells, Cells Mater. 1 (1991), 3–15.
10. J.E. Davies, B. Lowenberg and A. Shiga, The bone titanium interface in vitro, J. Biomed. Mater. Res. 24 (1990), 1289–1306.
11. B. Lowenberg, R. Chernecky, A. Shiga and J.E. Davies, Mineralized matrix production by osteoblasts on solid titanium in vitro, Cells Mater. 1 (1991), 177–187.
12. K.-L. Yao, R. Todescan, Jr. and J. Sodek, Temporal changes in matrix protein synthesis and mRNA expression during mineralized tissue formation by adult rat bone marrow cells in culture, J. Bone Miner. Res. 9 (1994), 231–240.
13. G.W. Brodland, A.T. Dolovich and J.E. Davies, Pretension critically affects the incremental strain field on pressure-loaded cell substrate membranes, J. Biomech. Eng. 114 (1992), 418–420.
14. C. Labarca and K. Paigen, A simple, rapid, and sensitive DNA assay procedure, Anal. Biochem. 102 (1980), 344–352.
15. T. Yoshikawa, H. Ohgushi, M. Okumura, S. Tamai, Y. Dohi and T. Moriyama, Biochemical and histological sequences of membranous ossification in ectopic site, Calcif. Tissue Int. 50 (1992), 184–188.
16. Y. Dohi, H. Ohgushi, S. Tabata, T. Yoshikawa, K. Dohi and T. Moriyama, Osteogenesis associated with bone Gla protein gene expression in diffusion chambers by bone marrow cells with demineralized bone matrix, J. Bone Miner. Res. 7 (1992), 1173–1180.
17. P.A. Price, J.W. Lothriner, S.A. Baukol and A.H. Reddi, Developmental appearance of the vitamin K-dependent protein of bone during calcification: analysis of mineralizing tissues in human, calf and rat, J. Biol. Chem. 256 (1981), 3781–3784.
18. P.A. Price, J.G. Parthemore and L.J. Deftos, New biochemical marker for bone metabolism. Measurement by radioimmunoassay of bone GLA protein in the plasma of normal subjects and patients with bone disease, J. Clin. Invest. 66 (1980), 878–883.
19. P.V. Hauschka and M.L. Reid, Timed appearance of a calcium-binding protein containing gamma-carboxyglutamic acid in developing chick bone, Dev. Biol. 65 (1978), 426–434.
20. T. Grange, J. Roux, G. Rigaud and R. Pictet, Two remote glucocorticoid responsive units interact cooperatively to promote glucocorticoid induction of rat tyrosine aminotransferase gene expression, Nucl. Acids Res. 17 (1989), 8695–8709.
21. M.P. Mark, W.T. Butler, C.W. Prince, R.D. Finkelman and J.V. Ruch, Developmental expression of 44-kDa bone phosphoprotein (osteopontin) and bone gamma-carboxyglutamic acid (Gla)-containing protein (osteocalcin) in calcifying tissues of rat, Differentiation 37 (1988), 123–136.
22. S.K. Nishimoto, C.H. Chang, E. Gendler, W.F. Stryker and M.E. Nimni, The effect of aging on bone formation in rats: biochemical and histological evidence for decreased bone formation capacity, Calcif. Tissue Int. 37 (1985), 617–624.
23. C.T. Brighton, B. Strafford, S.B. Gross, D.F. Leatherwood, J.L. Williams and S.R. Pollack, The proliferative and synthetic response of isolated calvarial bone cells of rats to cyclic biaxial mechanical strain, J. Bone Joint Surg. (Am.) 73 (1991), 320–331.
24. L.V. Harter, K.A. Hruska and R.L. Duncan, Human osteoblast-like cells respond to mechanical strain with increased bone matrix protein production independent of hormonal regulation, Endocrinology 136 (1995), 528–535.
25. R.L. Duncan and K.A. Hruska, Chronic, intermittent loading alters mechanosensitive channel characteristics in osteoblast-like cells, Am. J. Physiol. 267 (1994), F909–F916.
26. T. Yoshikawa, H. Ohgushi and S. Tamai, Immediate bone forming capability of prefabricated osteogenic hydroxyapatite, J. Biomed. Mater. Res. 32 (1996), 481–492.