Purification and the Effect of Manganese Ions on the Activity of Carboxymethylcellulases from Aspergillus niger and Cellulomonas biazotea

Folia Microbiologica

Volumn 42, Issue 4, 1997, Pages 303-311

  Siddiqui, K S ^a   Azhar, M J ^a   Rashid, M H ^a   Ghuri, T M ^a   Rajoka, M I ^a  

^a NATIONAL INSTITUTE FOR BIOTECHNOLOGY AND GENETIC ENGINEERING NIBGE   (Pakistan)

Author keywords

[No Author keywords available]

Indexed keywords

ASPERGILLUS NIGER; CELLULOMONAS BIAZOTEA;

BACTERIAL PROTEIN; CARBOXYMETHYLCELLULASE; CELLULASE; FUNGAL PROTEIN; GLYCOSIDASE; MANGANESE;

ACTINOMYCETALES; ARTICLE; ASPERGILLUS NIGER; BINDING SITE; CHEMISTRY; COMPARATIVE STUDY; DRUG EFFECT; ENZYMOLOGY; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; MOLECULAR WEIGHT; PROTEIN CONFORMATION; STRUCTURE ACTIVITY RELATION;

ACTINOMYCETALES; ASPERGILLUS NIGER; BACTERIAL PROTEINS; BINDING SITES; CELLULASE; FUNGAL PROTEINS; GLYCOSIDE HYDROLASES; KINETICS; MANGANESE; MOLECULAR WEIGHT; PROTEIN CONFORMATION; STRUCTURE-ACTIVITY RELATIONSHIP;

EID: 0031309363     PISSN: 00155632     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF02816940     Document Type: Article

References (26)

1. AKIBA S., KIMURA Y., YAMAMOTO K., KUMAGAI H.: Purification and characterization of a protease-resistant cellulase from Aspergillus niger. J.Ferment.Bioeng. 79, 125-130 (1995).
2. BAE H.D., MCALLISTER T.A., YANKE J., CHENG K.J., MUIR A.D.: Effects of condensed tannins on endoglucanase activity and filter paper digestion by Fibrobacter succinogenes S85. Appl.Environ.Microbiol. 59, 2132-2138 (1993).
3. BRADFORD M.M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal.Biochem. 72, 248-254 (1976).
4. CALZA R.E., IRWIN D.C., WILSON D.B.: Purification and characterization of two β-1,4-endoglucanases from Thermomonospora fusca. Biochemistry 24, 7797-7804 (1985).
5. COUGHLAN M.P.: Staining techniques for the detection of the individual components of cellulolytic enzyme system. Methods Enzymol. 160, 135-144 (1988).
6. DIXON M., WEBB E.C.: Enzyme inhibition and activation, pp. 332-467 in Enzymes. Academic Press, New York 1979.
7. FIEROBE H.P., BAGNARATARDIF C., GAUDIN C., GUERLESQUIN F., SAUVE P., BELAICH A., BELAICH J.P.: Purification and characterization of endoglucanase-C from Clostridium cellulolyticum - catalytic comparison with endoglucanase-A. Eur.J. Biochem. 217, 557-565 (1993).
8. GOKHALE D.V., PUNTAMBEKAR U.S., DEOBAGKAR D.N., PEBERDY J.F.: Production of cellulolytic enzyme by mutants of Aspergillus niger NCIM1207. Enzyme Microb. Technol. 10, 442-445 (1988).
9. KIM C.: Characterization and substrate specificity of an endo-β-1,4-D-glucanase 1 (avicelase 1) from an extracellular multi-enzyme complex of Bacillus circulans. Appl.Environ.Microbiol. 61, 959-965 (1995).
10. KUNDU R.K., DUBE S., DUBE D.K.: Extracellular cellulolytic enzyme system of Aspergillus japonicus. 3. Isolation, purification and characterization of multiple forms of endoglucanase. Enzyme Microb.Technol. 10, 100-109 (1988).
11. LINDNER W.A.: Carboxymethyl cellulase from Sclerotium rolfsii. Methods Enzymol. 160, 376-382 (1988).
12. LUPO D., STUTZENBERGER F.: Changes in endoglucanase patterns during growth of Thermomonospora curvata on cellulose. Appl.Environ.Microbiol. 54, 588-589 (1988).
13. MERRIL C.R.: Gel staining techniques. Methods Enzymol. 182, 477-488 (1990).
14. NAKAMURA K., KITAMURA K.: Purification and some properties of a cellulase active on crystalline cellulose from Cellulomonas uda. J.Ferment.Technol. 61, 379-382 (1983).
15. NG T.K., ZEIKUS J.G.: Endoglucanase from Clostridium thermocellum. Methods Enzymol. 160, 351-355 (1988).
16. RAJOKA M.I., MALIK K.A.: Comparison of different strains of Cellulomonas for production of cellulolytic and xylanolytic enzymes from biomass produced on saline lands. Biotechnol.Lett. 8, 753-756 (1986).
17. RANGARAJAN M., HARTLEY B.S.: Mechanism of D-fructose isomerization by Arthrobacter D-xylose isomerase. Biochem.J. 283, 223-233 (1992).
18. ROSSOMANDO E.F.: Ion-exchange chromatography. Methods Enzymol. 182, 309-317 (1990).
19. SAMI A.J., AKHTAR M.W.: Purification and characterization of two low-molecular weight endoglucanases of Cellulomonas flavigena. Enzyme Microb.Technol. 15, 586-592 (1993).
20. SAMI A.J., AKHTAR M.W., MALIK N.N., NAZ B.A.: Production of free and substrate-bound cellulases of Cellulomonas flavigena. Enzyme Microb.Technol. 10, 626-631 (1988).
21. SEE Y.P., JACKOWSKI G.: Estimating molecular weights of polypeptides by SDS-gel electrophoresis, pp. 1-21 in Protein Structure: A Practical Approach (T.E. Creighton, Ed.). IRL Press, Oxford (UK) 1989.
22. SEXANA S., BAHADUR J., VERMA A.: Effect of cobalt and nickle on growth and carboxymethylcellulase activity of Cellulomonas spp. Biometals S, 209-212 (1992).
23. SIDDIQUI K.S., LOVINY-ANDERTON T., RANGARAJAN M., HARTELY B.S.: Arthrobacter D-xylose isomerase: chemical modification of carboxyl groups and protein engineering of pH optimum. Biochem.J. 296, 685-691 (1993).
24. SIDDIQUI K.S., RASHID H., SHEMSI A.M., RAJOKA M.I.: A simple and nondestructive method for the separation of polysaccharides from β-glucosidase produced extracellularly by Aspergillus niger. Enzyme Microb.Technol. 16, 912-917 (1994).
25. STELLWAGEN E.: Gel filtration. Methods Enzymol. 182, 317-328 (1990).
26. WOOD T.M., BHAT K.M.: Methods for measuring cellulase activities. Methods Enzymol. 160, 87-112 (1988).