Use of an Agar-gel Technique for Large Scale Application to Recover Ascaris suum Larvae from Intestinal Contents of Pigs

Acta Veterinaria Scandinavica

Volumn 38, Issue 3, 1997, Pages 207-212

  Slotved, H C ^a   Barnes, E H ^a   Eriksen, L ^a   Roepstorff, A ^a   Nansen, P ^a   Bjorn H ^a  

^a UNIVERSITY OF COPENHAGEN   (Denmark)

Author keywords

migration; nematodea; recovery; worm

Indexed keywords

AGAR;

ANIMAL; ANIMAL DISEASE; ARTICLE; ASCARIASIS; ASCARIS SUUM; GEL; ISOLATION AND PURIFICATION; LARVA; PARASITOLOGY; PATHOLOGY; SMALL INTESTINE; SWINE; SWINE DISEASE; TIME;

AGAR; ANIMALS; ASCARIASIS; ASCARIS SUUM; GELS; INTESTINE, SMALL; LARVA; SWINE; SWINE DISEASES; TIME FACTORS;

EID: 0031301776     PISSN: 0044605X     EISSN: 17510147     Source Type: Journal    
DOI: 10.1186/BF03548483     Document Type: Article

References (13)

1. Arundel JH: Field procedure for counting gastrointestinal worms in sheep and cattle. Aust. vet. J., 1967, 43, 592–593. DOI: 10.1111/j.1751-0813.1967.tb04812.x
2. Bjorn H, Roepstorff A, Grondahl C, Eriksen L, Bjer-regaard J, Nansen P: Experimental transfer of adult Oesophagostomum dentatum from donor to helminth naive recipient pigs. A methodological study. J. Helminth., 1995, 69, 279–283. DOI: 10.1017/S0022149X0001484X
3. Daniel WD: Biostatistics: A foundation for analysis in the health sciences. 5th Edition, Wiley, New York, 1991, 318 pp.
4. Jergensen RJ: Isolation of infective Dictyocaulus larvae from herbage. Vet. Parasitol., 1975, 1, 61–67. DOI: 10.1016/0304-4017(75)90008-4
5. Jorgensen RJ, Nansen P, Nielsen K, Eriksen L, Andersen S: Experimental Ascaris suum infection in the pig. Population kinetics following low and high levels of primary infection in piglets. Vet. Parasitol., 1975, 1, 151–157. DOI: 10.1016/0304-4017(75)90017-5
6. Mwegoha WH, Jorgensen RJ: Recovery of infective 3rd stage larvae of Haemonchus contortus and Ostertagia ostertagi by migration in agar gel. Acta vet. scand., 1977, 18, 293–299.
7. Oksanen A, Eriksen L, Roepstorff A, Ilsø B, Nansen P, Lind P: Embryonation and infectivity of Ascaris suum eggs. A comparison of eggs collected from worm uteri with eggs isolated from pig faeces. Acta. vet. scand., 1990, 31, 393–398.
8. Roepstorff A, Eriksen L, Slotved H-C, Nansen P: Experimental Ascaris suum infection in the pig: Worm populations kinetics following single inoculation with three doses of infective eggs, (in press in Parasitology).
9. Slotved H-C, Barnes EH, Bjorn H, Christensen CM, Eriksen L, Roepstorff A, Nansen P: Recovery of Oesophagostomum dentatum from pigs by isolation of parasites migrating from large intestinal contents embedded in agar-gel. Vet. Parasitol., 1996, 63, 237–245. DOI: 10.1016/0304-4017(95)00916-7
10. SAS Institute Inc.: SAS/STAT Users Guide, Version 6, 4th Edition, 1990. Cary, NC, USA.
11. Van Wyk JA, Gerber HM: Recovery of nematodes from ruminants by migration from gastrointestinal ingesta and mucosa gelled in agar: preliminary report. Onderstepoort J. Vet. Res., 1978, 45, 29–38.
12. Van Wyk JA, Gerber HM, Groeneveld HT: A technique for the recovery of nematodes from ruminants by migration from gastro-intestinal ingesta gelled in agar: Large-scale application. Onderstepoort J. Vet. Res., 1980, 47, 147–158.
13. Van Wyk J A, Gerber HM, Alves RMR: Methods of infesting sheep with gastro-intestinal nematodes after cryopreservation: Dosing of larvae in gelatin capsules compared to dosing of larvae in water suspension. Onderstepoort J. Vet. Res., 1984, 51, 217–221.