A PCR-RFLP method for the detection and species identification of human microsporidia

Parassitologia

Volumn 39, Issue 4, 1997, Pages 437-439

  Sironi, M ^a   Bandi, C ^a   Novati, S ^b   Scaglia, M ^b  

^a UNIVERSITY OF MILAN   (Italy)

^b UNIVERSITY OF PAVIA   (Italy)

Author keywords

Diagnosis; Microsporidia; Opportunistic infections; PCR RFLP

Indexed keywords

PRIMER DNA; PROTOZOAL DNA;

AIDS RELATED COMPLEX; ANIMAL; ARTICLE; CLASSIFICATION; GENETICS; HUMAN; ISOLATION AND PURIFICATION; METHODOLOGY; MICROSPORIDA; MICROSPORIDIOSIS; PARASITOLOGY; POLYMERASE CHAIN REACTION; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; SPECIES DIFFERENCE;

AIDS-RELATED OPPORTUNISTIC INFECTIONS; ANIMALS; DNA PRIMERS; DNA, PROTOZOAN; HUMANS; MICROSPORIDA; MICROSPORIDIOSIS; POLYMERASE CHAIN REACTION; POLYMORPHISM, RESTRICTION FRAGMENT LENGTH; SPECIES SPECIFICITY;

EID: 0031301333     PISSN: 00482951     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (10)

1. Bandi C, Damiani G, Magrassi L, Grigolo A, Fani R, Sacchi L (1994). Flavobacteria as intracellular symbionts in cockroaches. Proc R Soc Lond B 257: 43-48.
2. Desportes-Livage I (1996). Human microsporidioses and AIDS: recent advances. Parasite 3: 107-113.
3. Fedorko DP, Hijazi YM (1996) Application of molecular techniques to the diagnosis of microsporidial infection Emerg Infect Dis 2: 183-192.
4. Katzwinkel-Wladarsh S, Lieb M, Loscher T, Rinder H. (1996) Direct amplification and species determination of microsporidian DNA from stool specimens Trop Med Intern Health 1: 373-378.
5. Liguory O, David F, Sarfati C, Schuitema ARJ, Hartskeerl RA, Derouin F, Modai J, Molina J-M (1997). Diagnosis of infections caused by Enterocytozoon bieneusi and Encephalitozoon intestinalis using polymerase chain reaction in stool specimens. AIDS 11: 723-726.
6. Maidak BL, Larsen N, McCaughey MJ, Overbeek R, Olsen GJ, Fogel K, Blandy J, Woese CR. (1995). The Ribosomal Database Project Nucleic Acids Res 22, suppl 3485-3487
7. Ombrouck C, Ciceron L, Biligui S, Brown S, Marechal P, Van Gool T, Datry A, Danis M, Desportes-Livage I (1997) Specific PCR assay for direct detection of intestinal microsporidia Enterocytozoon bieneusi and Encephalitozoon intestinalis in fecal specimens from human immunodeficiency virus-infected patients J Clin Microbiol 35: 652-655
8. Scaglia M, Sacchi L, Bernuzzi AM, De Piceis Polver P, Piacentini I, Concia GP, Da Silva AJ, Pieniazek NJ, Slemenda SB, Wallace S, Leitch GJ, Visvesvara GS (1995). Isolation and identification of Encephalitozoon hellem from an Italian AIDS patient with disseminated microsoporidiosis APMIS 102: 817-827.
9. Shadduck JA, Meccoli RA, Davis R, Font RL (1995) Isolation of a microsporidian from a human patient. J Infect Dis 162: 773-776.
10. Weber R, Bryan RT, Schwartz DA, Owen RL (1995) Human microsporidial infections. Clin Microbiol Rev 7: 426-461.