Continuous Cultivation of Bakers' Yeast: Change in Cell Composition at Different Dilution Rates and Effect of Heat Stress on Trehalose Level

Folia Microbiologica

Volumn 42, Issue 5, 1997, Pages 463-467

  Ertugay, N ^a   Hamamci, H ^a  

^a MIDDLE EAST TECHNICAL UNIVERSITY   (Turkey)

Author keywords

[No Author keywords available]

Indexed keywords

FUNGAL PROTEIN; FUNGAL RNA; GLYCOGEN; TREHALOSE;

ARTICLE; BACTERIAL COUNT; BIOMASS; CARBOHYDRATE METABOLISM; FERMENTATION; GROWTH, DEVELOPMENT AND AGING; HEAT SHOCK RESPONSE; METABOLISM; SACCHAROMYCES CEREVISIAE;

BIOMASS; CARBOHYDRATE METABOLISM; COLONY COUNT, MICROBIAL; FERMENTATION; FUNGAL PROTEINS; GLYCOGEN; HEAT-SHOCK RESPONSE; RNA, FUNGAL; SACCHAROMYCES CEREVISIAE; TREHALOSE;

EID: 0031300709     PISSN: 00155632     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF02826554     Document Type: Article

References (16)

1. DUBOIS M., GILLES K.A., HAMILTON J.K., REBERS P.A., SMITH F.: Colorimetric method for determination of sugars and related substances. Anal.Chem. 28, 350-356 (1956).
2. European Brewery Convention - Anatytica Microbiologica. J.Inst.Brew. 83, 109-118 (1977).
3. HARTREE E.F.: Determination of protein: A modification of the Lowry method that gives a linear photometric response. Anal. Biochem. 48, 422-427 (1972).
4. HERBERT D., PHIPPS P.J., STRANGE R.E.: Chemical analysis of microbial cells, pp. 209-344 in Methods in Microbiology (J.R. Norris, D.W. Ribbons, Eds), Vol. 58. Academic Press, London 1971.
5. KÜENZI M.T., FIECHTER A.: Regulation of carbohydrate composition of Saccharomyces cerevisiae under growth limitation. Arch.Microbiol. 84, 254-265 (1972).
6. LILLIE S.H., PRINGLE J.R: Reserve carbohydrate metabolism in Saccharomyces cerevisiae. Response to nutrient limitation. J.Bacteriol. 143, 1384-1394 (1980).
7. LONDESBOROUGH J., VUORIO O.E.: Purification of trehalose synthase from bakers' yeast. Its temperature-dependent activation by fructose-6-phosphate and inhibition by phosphate. Eur.J.Biochem. 216, 841-848 (1993).
8. NEVES M.J., FRANÇOIS J.: On the mechanism by which a heat shock induces trehalose accumulation in Saccharomyces cerevisiae. Biochem.J. 288, 859-864 (1992).
9. PANEK A.C., MANSURE-VANIA J.J., PASCHOALIN M.F., PANEK D.: Regulation of trehalose metabolism in Saccharomyces cerevisiae mutants during temperature shifts. Biochimie 72, 77-79 (1990).
10. PANEK A.D., MATTOON J.R.: Regulation of energy metabolism in Saccharomyces cerevisiae. Arch.Biochem.Biophys. 183, 306-316 (1977).
11. PEJIN D., RAZMOVSKI R.: Continuous cultivation on the yeast Saccharomyces cerevisiae at different dilution rates and glucose concentrations in nutrient media. Folia Microbiol. 38, 141-146 (1993).
12. RAO P., PATTABIRAMAN T.N.: Reevaluation of the phenol-sulfuric acid reaction for the estimation of hexoses and pentoses. Anal.Biochem. 181, 18-22 (1989).
13. RIBEIRO M.J.S., SILVA J.T., PANEK A.D.: Trehalose metabolism in Saccharomyces cerevisiae during heat shock. Biochim.Biophys. Acta 1200, 139-147 (1994).
14. STEWART P.R.: Analytical methods for yeasts, pp. 11-145 in Methods in Cellular Biology (D.W. Prescott, Ed.), Vol. 12. Academic Press, New York 1975.
15. DE VIRGILIO C., SIMMEN U., HOTTIGER T., BÖLLER T., WIEMKEN A.: Heat shock induces enzymes of trehalose metabolism, trehalose accumulation, and thermotolerance in Schizosaccharomyces pombe, even in the presence of cycloheximide. FEBS Lett. 273, 107-110 (1990).
16. WINKLER K., KIENLE I., BÜRGERT M., WAGNER J.C., HOLZER H.: Metabolic regulation of the trehalose content of vegetative yeast. FEBS Lett. 291, 269-272 (1991).