Quantitative videomicrofluorometry: Empirical methods for an automatic determination of the thresholding level for fluorescent objects. Application to living lymphoblastoid cell lines

Proceedings of SPIE - The International Society for Optical Engineering

Volumn 3197, Issue , 1997, Pages 194-204

  Vigo, Jean ^a   Viallet, Pierre ^a   Salmon, Jean Marie ^a  

^a UNIVERSITÉ DE PERPIGNAN   (France)

Author keywords

Automatic protocol of correction; Fluorescent probes; Living cells; Nucleus and plasmic membrane labelling; Numerical Image Analysis; Thresholding level

Indexed keywords

CELLS; FLUORESCENCE; IMAGE RECORDING; MEDICAL IMAGING; SPECTROSCOPIC ANALYSIS;

VIDEOMICROFLUOROMETRY;

IMAGE ANALYSIS;

EID: 0031289139     PISSN: 0277786X     EISSN: None     Source Type: Conference Proceeding    
DOI: 10.1117/12.297966     Document Type: Conference Paper

References (7)

1. Multiparametric investigations for studiing the time dependence of the effect of Adriamycin on some human leukemic lymphoblasts using multiwavelength videmicrofluorometry
2. Nile Red labelling of single living cells for contour delineation to quantify and evaluate the distribution of R123 with fluorescence image cytometry
3. Altered surface membrane glycoprotein in Vinca Alkaloïd-resistant human leukemic lymphoblasts
4. Fluorescence image cytometry: From qualitative to quantitative measurements