Enzyme kinetics on a molecular level with optical microscopy

Proceedings of SPIE - The International Society for Optical Engineering

Volumn 3197, Issue , 1997, Pages 134-139

  Uhl, Volker ^a   Pilarczyk, Götz ^a   Greulich, Karl Otto ^a  

^a FRITZ LIPMANN INSTITUTE   (Germany)

Author keywords

Enzyme catalysis; Enzyme kinetics; Fluorescence; Optical microscopy; Single molecule detection

Indexed keywords

CATALYSIS; ENZYME KINETICS; ENZYMES; FLUORESCENCE; OPTICAL MICROSCOPY; REACTION KINETICS; SUBSTRATES;

ENZYME CATALYSIS; MOLECULE DETECTION;

BIOTECHNOLOGY;

EID: 0031288891     PISSN: 0277786X     EISSN: None     Source Type: Conference Proceeding    
DOI: 10.1117/12.297961     Document Type: Conference Paper

References (6)

1. Differences in the chemical reactivity of individual molecules of an enzyme
2. Laser manipulation and UV induced single molecule reactions of individual DNA molecules
3. Imaging of single fluorescent molecules and individual ATP turnovers by single myosin molecules in aqueous solution
4. Direct measurement of single molecule diffusion and photodecomposition in free solution
5. Single molecule imaging of fluophores and enzymatic reactions achieved by objective-type total internal reflection microscopy