Cloning and characterisation of a cytosolic glutathione reductase cDNA from pea (Pisum sativum L.) and its expression in response to stress

Plant Molecular Biology

Volumn 35, Issue 5, 1997, Pages 641-654

  Stevens, Rebecca G ^a   Creissen, Gary P ^a   Mullineaux, Philip M ^a  

^a JOHN INNES CENTRE   (United Kingdom)

Author keywords

Chilling; Drought; Glutathione reductase; Isoforms; Oxidative stress; Pisum sativum

Indexed keywords

COMPLEMENTARY DNA; GLUTATHIONE REDUCTASE; HYBRID PROTEIN; MESSENGER RNA; PLANT DNA; PLANT RNA;

AMINO ACID SEQUENCE; ARTICLE; COLD; CYTOSOL; DESICCATION; DNA SEQUENCE; ENZYMOLOGY; ESCHERICHIA COLI; GENE EXPRESSION REGULATION; GENETICS; MOLECULAR CLONING; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PEA; PHYSIOLOGY; RESTRICTION MAPPING; SEQUENCE HOMOLOGY;

AMINO ACID SEQUENCE; BASE SEQUENCE; CLONING, MOLECULAR; COLD; CYTOSOL; DESICCATION; DNA, COMPLEMENTARY; DNA, PLANT; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, PLANT; GLUTATHIONE REDUCTASE; MOLECULAR SEQUENCE DATA; OPEN READING FRAMES; PEAS; RECOMBINANT FUSION PROTEINS; RESTRICTION MAPPING; RNA, MESSENGER; RNA, PLANT; SEQUENCE ANALYSIS, DNA; SEQUENCE HOMOLOGY, AMINO ACID; SEQUENCE HOMOLOGY, NUCLEIC ACID;

ESCHERICHIA COLI; PISUM SATIVUM; SATIVUM;

EID: 0031282640     PISSN: 01674412     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1005858120232     Document Type: Article

References (69)

1. Alscher RG: Biosynthesis and antioxidant function of glutathione in plants. Physiol Plant 77: 457-464 (1989).
2. Anderson JV, Hess JL, Chevone BI: Purification, characterization, and immunological properties for two isoforms of glutathione reductase from eastern white pine needles. Plant Physiol 94: 1402-1409 (1990).
3. Anderson MD, Prasad TK, Stewart CR: Changes in isozyme profiles of catalase, peroxidase, and glutathione reductase during acclimation to chilling in mescotyls of maize seedlings. Plant Physiol 109: 1247-1257 (1995).
4. Aono M, Saji H, Fujiyama K, Sugita M, Kondo N, Tanaka K: Decrease in activity of glutathione reductase enhances paraquat sensitivity in transgenic Nicotiana tabacum. Plant Physiol 107: 645-648 (1995).
5. Asada K: Production and action of active oxygen species in photosynthetic tissues. In: Foyer CH, Mullineaux PM (eds) Causes of Photooxidative Stress and Amelioration of Defense Systems in Plants, pp. 77-104. CRC Press, Boca Raton, FL (1994).
6. Bielawski W, Joy KW: Reduced and oxidised glutathione and glutathione-reductase activity in tissues of Pisum sativum. Planta 169: 267-272 (1986).
7. Bielawski W, Joy KW: Properties of glutathione reductase from chloroplasts and roots of pea. Phytochemistry 25: 2261-2265 (1986).
8. Blackwell JR, Morgan R: A novel strategy for production of a highly expressed recombinant protein in an active form. FEBS Lett 295: 10-12 (1991).
9. Bradford MM: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248-254 (1976).
10. Broadbent P, Creissen GP, Kular B, Wellburn AR, Mullineaux PM: Oxidative stress responses in transgenic tobacco containing altered levels of glutathione reductase activity. Plant J 8: 247-255 (1995).
11. Collinson LP, Dawes IW: Isolation, characterization and over-expression of the yeast gene, GLR1, encoding glutathione reductase. Gene 156: 123-127 (1995).
12. Conklin PL, Last RL: Differential accumulation of antioxidant mRNAs in Arabidopsis thaliana exposed to ozone. Plant Physiol 109: 203-212 (1995).
13. Connell JP, Mullet JE: Pea chloroplast glutathione reductase: purification and characterization. Plant Physiol 82: 351-356 (1986).
14. Creissen G, Edwards EA, Enard C, Wellburn A, Mullineaux P: Molecular characterization of glutathione reductase cDNAs from pea (Pisum sativum L.). Plant J 2: 129-131 (1992).
15. Creissen G, Reynolds H, Xue Y, Mullineaux P: Simultaneous targeting of pea glutathione reductase and of a bacterial fusion protein to chloroplasts and mitochondria in transgenic tobacco. Plant J 8: 167-175 (1995).
16. Creissen GP, Edwards EA, Mullineaux PM: Glutathione reductase and ascorbate peroxidase. In: Foyer CH, Mullineaux PM (eds) Causes of Photooxidative Stress and Amelioration of Defense Systems in Plants, pp. 343-364. CRC Press, Boca Raton (1994).
17. Creissen GP, Mullineaux PM: Cloning and characterisation of glutathione reductase cDNAs and identification of two genes encoding the tobacco enzyme. Planta 197: 422-425 (1995).
18. Day W: A direct reading continuous flow porometer. Agric Meteorol 18: 81-89 (1977).
19. Dear S, Staden R: A sequence assembly and editing program for efficient management of large projects. Nucl Acids Res 19: 3907-3911 (1991).
20. Devereux J, Haeberli P, Smithies O: A comprehensive set of sequence analysis programs for the VAX. Nucl Acids Res 12: 387-395 (1984).
21. Dhindsa RS: Drought stress, enzymes of glutathione metabolism, oxidation injury, and protein synthesis in Tortula ruralis. Plant Physiol 95: 648-651 (1991).
22. Drumm-Herrel H, Gerhäusser U, Mohr H: Differential regulation by phytochrome of the appearance of plastidic and cytoplasmatic isoforms of glutathione reductase in mustard (Sinapis alba L.) cotyledons. Planta 178: 103-109 (1989).
23. Edwards EA, Enard C, Creissen GP, Mullineaux PM: Synthesis and properties of glutathione reductase in stressed peas. Planta 192: 137-143 (1994).
24. Edwards EA, Rawsthorne S, Mullineaux PM: Subcellular distribution of multiple forms of glutathione reductase in leaves of pea (Pisum sativum L.). Planta 180: 278-284 (1990).
25. Ellis THN, Hellens RP, Turner L, Lee D, Domoney C, Welham T: On the pea linkage map. Pisum Genet 25: 5-12 (1993).
26. Ellis THN, Turner L, Hellens RP, Lee D, Harker CL, Enard C, Domoney C, Davies DR: Linkage maps in pea. Genetics 130: 649-663 (1992).
27. Elstner EF: Oxygen activation and oxygen toxicity. Annu Rev Plant Physiol 33: 73-96 (1982).
28. Feinberg AP, Vogelstein B: A technique for radiolabeling DNA-restriction endonuclease fragments to high specific activity. Anal Biochem 132: 6-13 (1983).
29. Fontaine O, Huault C, Pavis N, Billard J-P: Dormancy breakage of Hordeum vulgare seeds: effects of hydrogen peroxide and scarification on glutathione level and glutathione reductase activity. Plant Physiol Biochem 32: 677-683 (1994).
30. Foyer CH, Halliwell B: The presence of glutathione and glutathione reductase in chloroplasts: a proposed role in ascorbic acid metabolism. Planta 133: 21-25 (1976).
31. Frohman MA, Dush MK, Martin GR: Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer. Proc Natl Acad Sci USA 85: 8998-9002 (1988).
32. Genty B, Briantais J-M, Baker NR: The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence. Biochim Biophys Acta 990: 87-92 (1989).
33. Gillham DJ, Dodge AD: Hydrogen-peroxide-scavenging systems within pea chloroplasts. Planta 167: 246-251 (1986).
34. Greer S, Perham RN: Glutathione reductase from Escherichia coli: cloning and sequence analysis of the gene and relationship to other flavoprotein disulfide oxidoreductases. Biochemistry 25: 2736-2742 (1986).
35. Guy CL, Carter JV: Characterization of partially purified glutathione reductase from cold-hardened and nonhardened spinach leaf tissue. Cryobiology 21: 454-464 (1984).
36. Hausladen A, Alscher RG: Cold-hardiness-specific glutathione reductase isozymes in red spruce. Plant Physiol 105: 215-223 (1994).
37. Hellens RP, Ellis THN, Lee D, Turner L: Repeated sequences as genetic markers in pooled tissue samples. Plant Mol Biol 22: 153-157 (1993).
38. Jahnke LS, Hull MR, Long SP: Chilling stress and oxygen metabolizing enzymes in Zea mays and Zea diploperennis. Plant Cell Environ 14: 97-104 (1991).
39. Jiang F, Hellman U, Sroga GE, Bergman B, Mannervik B: Cloning, sequencing, and regulation of the glutathione reductase gene from the cyanobacterium Anabaena PCC 7120. J Biol Chem 270: 22882-22889 (1995).
40. Karpinski S, Escobar C, Karpinska B, Creissen G, Mullineaux PM: Photosynthetic electron transport regulates the expression of cytosolic ascorbate peroxidase genes in Arabidopsis during excess light stress. Plant Cell 9: 627-640 (1997).
41. Karpinski S, Wingsle G, Karpinska B, Hällgren J-E: Molecular responses to photooxidative stress in Pinus sylvestris (L.). Plant Physiol 103: 1385-1391 (1993).
42. Klapheck S, Zimmer I, Cosse H: Scavenging of hydrogen peroxide in the endosperm of Ricinus communis by ascorbate peroxidase. Plant Cell Physiol 31: 1005-1013 (1990).
43. Kubo A, Sano T, Saji H, Tanaka K, Kondo N, Tanaka K: Primary structure and properties of glutathione reductase from Arabidopsis thaliana. Plant Cell Physiol 34: 1259-1266 (1993).
44. Kullik I, Storz G: Transcriptional regulators of the oxidative stress response in prokaryotes and eukaryotes. Redox Rep 1: 23-29 (1994).
45. Madamanchi NR, Anderson JV, Alscher RG, Cramer CL, Hess JL: Purification of multiple forms of glutathione reductase from pea (Pisum sativum L.) seedlings and enzyme levels in ozonefumigated pea leaves. Plant Physiol 100: 138-145 (1992).
46. Mapson LW, Isherwood FA: Glutathione reductase from germinated peas. Biochem J 86: 173-191 (1963).
47. Mehlhorn H: Ethylene-promoted ascorbate peroxidase activity protects plants against hydrogen peroxide, ozone and paraquat. Plant Cell Environ 13: 971-976 (1990).
48. Mittl PRE, Schulz GE: Structure of glutathione reductase from Escherichia coli at 1.86 Å resolution: comparison with the enzyme from human erythrocytes. Prot Sci 3: 799-809 (1994).
49. Mittler R, Zilinskas BA: Regulation of pea cytosolic ascorbate peroxidase and other antioxidant enzymes during the progression of drought stress and following recovery from drought. Plant J 5: 397-405 (1994).
50. Mullineaux P, Enard C, Hellens R, Creissen G: Characterisation of a glutathione reductase gene and its genetic locus from pea (Pisum sativum L.). Planta 200: 186-194 (1996).
51. O'Kane D, Gill V, Boyd P, Burdon R: Chilling, oxidative stress and antioxidant responses in Arabidopsis thaliana callus. Planta 198: 371-377 (1996).
52. Olsen LJ, Harada JJ: Peroxisomes and their assembly in higher plants. Ann Rev Plant Physiol Plant Mol Biol 46: 123-146 (1995).
53. Prasad TK, Anderson MD, Martin BA, Stewart CR: Evidence for chilling-induced oxidative stress in maize seedlings and a regulatory role for hydrogen peroxide. Plant Cell 6: 65-74 (1994).
54. Price AH, Hendry GAF: Iron-catalysed radical formation and its possible contribution to drought damage in nine native grasses and three cereals. Plant Cell Environ 14: 477-484 (1991).
55. Reed DJ: Nitrosoureas. In: Sies H (ed) Oxidative Stress, pp. 115-130. Academic Press, London (1985).
56. Salin ML: Toxic oxygen species and protective systems of the chloroplast. Physiol Plant 72: 681-689 (1988).
57. Sambrook J, Fritsch EF, Maniatis T: Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY (1989).
58. Scandalios JG: Oxygen stress and Superoxide dismutases. Plant Physiol 101: 7-12 (1993).
59. Schirmer RH, Krauth-Siegel RL, Schulz GE: Glutathione reductase. In: Dolphin D, Poulson R, Avramovic O (eds) Coenzymes and Cofactors, pp. 553-596. John Wiley, New York (1989).
60. Serrano A, Llobell A: Occurence of two isoforms of glutathione reductase in the green alga Chlamydomonas reinhardtii. Planta 190: 199-205 (1993).
61. - production in thylakoid membranes under water stress. Physiol Plant 87: 211-216 (1993).
62. Smirnoff N: The role of active oxygen in the response of plants to water deficit and desiccation. New Phytol 125: 27-58 (1993).
63. Smith DB, Johnson KS: Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase. Gene 67: 31-40 (1988).
64. Smith IK, Vierheller TL, Thorne CA: Assay of glutathione reductase in crude tissue homogenates using 5,5′-dithiobis(2-nitrobenzoic acid). Anal Biochem 175: 408-413 (1988).
65. Taniguchi M, Hara T, Honda H: Similarities between rat liver mitochondrial and cytosolic glutathione reductases and their apoenzyme accumulation in riboflavin deficiency. Biochem Int 13: 447-454 (1986).
66. Tsang EWT, Bowler C, Hérouart D, Van Camp W, Villarroel R, Genetello C, Van Montagu M, Inzé D: Differential regulation of superoxide dismutases in plants exposed to environmental stress. Plant Cell 3: 783-792 (1991).
67. Tutic M, Lu X, Schirmer RH, Werner D: Cloning and sequencing of mammalian glutathione reductase cDNA. Eur J Biochem 188: 523-528 (1990).
68. Veres G, Gibbs RA, Scherer SE, Caskey CT: The molecular basis of the sparse fur mouse mutation. Science 237: 415-417 (1987).
69. Wingsle G, Karpinski S: Differential redox regulation by glutathione of glutathione reductase and CuZn-Superoxide dismutase gene expression in Pinus sylvestris L. needles. Planta 198: 151-157 (1996).