Differential modification of flavonoid and isoflavonoid biosynthesis with an antisense chalcone synthase construct in transgenic Lotus corniculatus

Plant Molecular Biology

Volumn 35, Issue 4, 1997, Pages 509-522

  Colliver, S P ^a,b   Morris, P ^a   Robbins, M P ^a  

^a ABERYSTWYTH UNIVERSITY   (United Kingdom)

^b UNILEVER RESEARCH   (United Kingdom)

Author keywords

Agrobacterium rhizogenes; Antisense RNA; Condensed tannins; Genetic compensation; Heterologous gene; Lotus corniculatus; Phytoalexin

Indexed keywords

ACYLTRANSFERASE; CHALCONE SYNTHASE; COMPLEMENTARY DNA; FLAVONOID; ISOFLAVONE DERIVATIVE; MESSENGER RNA; PHYTOALEXINS; PLANT EXTRACT; PLANT RNA; PROANTHOCYANIDIN; RECOMBINANT DNA; RHATANNIN; TANNIN DERIVATIVE; TERPENE;

ARTICLE; BIOSYNTHESIS; CHEMICAL MODEL; CULTURE TECHNIQUE; ENZYMOLOGY; GENE DOSAGE; GENETICS; LEGUME; MEDICINAL PLANT; METABOLISM; PHYSIOLOGY; PLANT ROOT; TRANSGENE; TRANSGENIC PLANT;

ACYLTRANSFERASES; CULTURE TECHNIQUES; DNA, ANTISENSE; DNA, RECOMBINANT; FABACEAE; FLAVONOIDS; GENE DOSAGE; ISOFLAVONES; MODELS, CHEMICAL; PLANT EXTRACTS; PLANT ROOTS; PLANTS, GENETICALLY MODIFIED; PLANTS, MEDICINAL; PROANTHOCYANIDINS; RNA, MESSENGER; RNA, PLANT; TANNINS; TERPENES; TRANSGENES;

AGROBACTERIUM RHIZOGENES; AVES; LOTUS; LOTUS CORNICULATUS; PHASEOLUS VULGARIS;

EID: 0031282616     PISSN: 01674412     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1005821801228     Document Type: Article

References (53)

1. An C, Ichinose Y, Yamada T, Tanaka Y, Shiraishi T, Oku H: Organization of the genes encoding chalcone synthase in Pisum sativum. Plant Mol Biol 21: 789-803 (1993).
2. Bailey JA, Mansfield JW: Phytoalexins. Blackie, Glasgow (1982).
3. Beffa RS, Neuhaus J-M, Meins F: Physiological compensation in antisense transformants: Specific induction of an 'ersatz' glucan endo-1,3-glucosidase in plants infected with necrotizing viruses. Proc Natl Acad Sci USA 90: 8792-8796 (1993).
4. van Blokland R, de Lange P, Mol JNM, Kooter JM: Modulation of gene expression in plants by antisense genes. In Crooke ST, Lebleu B (eds) Antisense Research and Applications, pp. 125-148. CRC Press, Boca Raton, FL (1993).
5. Blount JW, Dixon RA, Paiva NL: Stress responses in alfalfa (Medicago sativa L.). XVI. Antifungal activity of medicarpin and its biosynthetic precursors; implications for the genetic manipulation of stress metabolites. Physiol Mol Plant Path 41: 333-349 (1992).
6. Bonde MR, Miller RL, Ingham JL: Induction and identification of sativan and vestitol as two phytoalexins from Lotus corniculatus. Phytochemistry 12: 2957-2959 (1973).
7. Carron TR, Robbins MP, Morris P: Genetic modification of condensed tannin biosynthesis in Lotus corniculatus. I. Heterologous antisense dihydroflavonol reductase down-regulates tannin accumulation in 'hairy root' cultures. Theor Appl Genet 87: 1006-1015 (1994).
8. Cesarone CF, Bolognesi C, Santi L: Improved microfluorometric DNA determination in biological material using 33258 Hoescht. Anal Biochem 100: 188-197 (1979).
9. Colliver SP, Robbins MP, Morris P: An antisense strategy for the genetic manipulation of condensed tannin and isoflavonoid phytoalexin accumulation in transgenic Lotus corniculatus L. Acta Hort 381: 148-151 (1994).
10. Cramer CL, Edwards K, Dron M, Liang X, Dildine SL, Bolwell GP, Dixon RA, Lamb CJ, Schuch W: Phenylalanine ammonialyase gene organisation and structure. Plant Mol Biol 12: 367-383 (1989).
11. Dalkin K, Edwards R, Edington B, Dixon RA: Stress responses in alfalfa (Medicago sativa L.). I. Induction of phenylpropanoid biosynthesis and hydrolytic enzymes in elicitor-treated cell suspension cultures. Plant Physiol 92: 440-446 (1990).
12. Dharmatilake AJ, Bauer WD: Chemotaxis of Rhizobium meliloti towards nodulation gene-inducing compounds from alfalfa roots. Appl Environ Microbiol 58: 1153-1158 (1992).
13. Elomaa P, Honkanen J, Puska R, Seppänen P, Helariutta Y, Mehto M, Kotilainen M, Nevalainen L, Teeri TH: Agrobacterium-mediated transfer of antisense chalcone synthase cDNA to Gerbera hybrida inhibits flower pigmentation. Bio/technology 11: 508-511 (1993).
14. Estabrook EM, Sengupta-Gopalan C: Differential expression of phenylalanine ammonia-lyase and chalcone synthase during soybean nodule deveolpment. Plant Cell 3: 299-308 (1991).
15. Feinbaum RL, Ausubel FM: Transcriptional regulation of the Arabidopsis thaliana chalcone synthase gene. Mol Cell Biol 8: 1985-1992 (1988).
16. Gamborg OL, Miller RA, Ojima K: Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50: 151-158 (1968).
17. Grayer RJ, Kimmins FM, Padgham DE, Harborne JB, Ranga Rao DV: Condensed tannin levels and resistance of groundnuts (Arachis hypogaea) against Aphis craccivora. Phytochemistry 31: 3795-3800 (1992).
18. Hahlbrock K, Scheel D: Physiology and molecular biology of phenylpropanoid metabolism. Annu Rev Plant Physiol Plant Mol Biol 40: 347-369 (1989).
19. Halpin C, Knight ME, Foxon GA, Campbell MM, Boudet AM, Boon JJ, Chabbert B, Tollier M-T, Schuch W: Manipulation of lignin quality by down-regulation of cinnamyl alcohol dehydrogenase. Plant J 6: 339-350 (1994).
20. Harborne JB: Introduction to Ecological Biochemistry, 4th ed. Academic Press, London. (1993).
21. Harker CL, Noel-Ellis TH, Coen ES: Identification and genetic regulation of the chalcone synthase multigene family in pea. Plant Cell 2: 185-194 (1990).
22. Hedin PA, Waage SK: Roles of flavonoids in plant resistance to insects. In: Cody V, Middleton E, Harborne JB (eds) Plant Flavonoids in Biology and Medicine, vol. 1. Biochemical, Pharmacological and Structure-Activity Relationships, pp. 87-100. Alan R. Liss, New York. (1986).
23. Heller W, Forkmann G: Biosynthesis. In: Harborne JB (ed) The Flavonoids, pp. 399-425. Chapman and Hall, London (1988).
24. Hemon P, Robbins MP, Cullimore JV: Targeting of glutamine synthetase to the mitochondria of transgenic tobacco. Plant Mol Biol 15: 895-904 (1990).
25. Herrmann A, Shulz W, Hahlbrock K: Two alleles of the single-copy chalcone synthase gene in parsley differ by a transposon-like element. Mol Gen Genet 212: 95-98 (1988).
26. Hirel B, Marsolier MC, Hoarau A, Hoarau J, Brangeon J, Schafer R, Verma DPS: Forcing expression of soybean root glutamine synthetase gene in tobacco induces a native gene encoding cytosolic enzyme. Plant Mol Biol 20: 207-218 (1992).
27. Ingham JL: A further investigation of phytoalexin formation in the genus Trifolium. Z Naturforsch 45c: 829-834 (1990).
28. Jacobs M, Rubery PH: Naturally occurring auxin transport regulators. Science 241: 346-349 (1988).
29. Jorgensen R: The genetic origins of biosynthesis and light-responsive control of the chemical UV screen of land plants. In: Ellis BE, Kuroki GW, Stafford HA (eds) Genetic Engineering of Plant Secondary Metabolism, pp. 179-192. Plenum Press, New York. (1994).
30. Junghans H, Dalkin K, Dixon RA: Stress responses in alfalfa (Medicago saliva L.). 15. Characterization and expression patterns of members of a subset of the chalcone synthase multigene family. Plant Mol Biol 22: 239-253 (1993).
31. Koes RE, Spelt CE, Mol JNM: The chalcone synthase multigene family of Petunia hybrida (V30): differential, light-regulated expression during flower development and UV light induction. Plant Mol Biol 12: 213-225 (1989).
32. van der Krol AR, Stuitje AR, Mol JNM: Modulation of floral pigmentation by antisense technology. In: Mol JNM, van der Krol AR (eds) Antisense nucleic acids and proteins, pp. 125-140. Marcel Dekker, New York (1991).
33. Lamb CJ, Lawton MA, Dron M, Dixon RA: Signals and transduction mechanisms for activation of plant defense against microbial attack. Cell 56: 215-224 (1989).
34. Lawton MA, Lamb CJ: Transcriptional activation of plant defence genes by fungal elicitor, wounding and infection. Mol Cell Biol 7: 335-341 (1987).
35. Middleton EM, Teramura AH: The role of flavonol glycosides and carotenoids in protecting soybean from ultraviolet damage. Plant Physiol 103: 741-752 (1993).
36. Mol JNM, van der Krol AR, van Tunen AJ, van Blokland R, de Lange P, Stuitje AR: Regulation of plant gene-expression by antisense RNA. FEBS Lett 268: 427-430 (1990).
37. Morris P, Robbins MP: Condensed tannin formation by Agrobacterium rhizogenes transformed root and shoot organ cultures of Lotus corniculatus. J Exp Bot 43: 221-231 (1992).
38. Ougham HJ, Davies TGE: Leaf development in Lolium temulentum: gradients of RNA complement plastid and non-plastid transcripts. Physiol Plant 79: 331-338 (1990).
39. Re EB, Jones D, Learned RM: Co-expression of native and introduced genes reveals cryptic regulation of HMG CoA reductase expression in Arabidopsis. Plant J 7: 771-784 (1995).
40. Robbins MP, Carron TR, Morris P: Transgenic Lotus corniculatus: a model system for the modification and genetic manipulation of condensed tannin biosynthesis. In: Hemingway RW, Laks PE (eds) Plant Polyphenols, pp. 111-131. Plenum Press, New York. (1992).
41. Robbins MP, Carron TR, Webb KJ: Detecting transgenes in Lotus corniculatus using the polymerase chain reaction. Lotus Newsl 24: 45-48 (1993).
42. Robbins MP, Evans TE, Morris P, Carron TR: Some notes on the extraction of genomic DNA from transgenic Lotus. Lotus Newsl 22: 18-21 (1991).
43. Robbins MP, Hartnoll J, Morris P: Phenylpropanoid defence responses in transgenic Lotus corniculatus. I. Glutathione elicitation of isoflavan phytoalexins in transformed root cultures. Plant Cell Rep 10: 59-62 (1991).
44. Robbins MP, Thomas B, Morris P: Phenylpropanoid defence responses in transgenic Lotus corniculatus. II. Modelling plant defence responses in transgenic root cultures using thiol and carbohydrate elicitors. J Exp Bot 46: 513-524 (1995).
45. Ryder TB, Hedrick SA, Bell JN, Liang X, Clouse SD, Lamb CJ: Organisation and differential activation of a gene family encoding the plant defence enzyme chalcone synthase in Phaseolus vulgaris. Mol Gen Genet 210: 219-233 (1987).
46. Salehuzzaman SNIM, Jacobsen E, Visser RGF: Isolation and characterization of a cDNA encoding granule-bound starch synthase in cassava (Manihot esculenta Crantz) and its antisense expression in potato. Plant Mol Biol 23: 947-962 (1993).
47. Sallaud C, El-Turk J, Bigarré L, Sevin H, Welle R, Esnault R: Nucleotide sequences of three chalcone reductase genes from alfalfa. Plant Physiol 108: 869-870 (1995).
48. Scalbert A: Antimicrobial properties of tannins. Phytochemistry 30: 3875-3883 (1991).
49. Stafford HA, Cheng T-Y: The procyanidins of douglas fir seedlings, callus, and cell suspension cultures derived from cotyledons. Phytochemistry 19: 131-135 (1980).
50. Todd JJ, Vodkin LO: Duplications that suppress and deletions that restore expression from a chalcone synthase multigene family. Plant Cell 8: 687-699 (1996).
51. van Tunen AJ, Koes RE, Spelt CE, van der Krol AR, Stuitje AR, Mol JNM: Cloning of the two chalcone flavanone isomerase genes from Petunia hybrida: coordinate, light-regulated and differential expression of flavonoid genes. EMBO J 7: 1257-1263 (1988).
52. Wingender R, Roehrig H, Hoericke C, Wing D, Schell J: Differential regulation of soybean chalcone synthase genes in plant defence, symbiosis and upon environmental stimuli. Mol Gen Genet 218: 315-322 (1989).
53. Zerback R, Dressler K, Hess D: Flavonoid compounds from pollen and stigma of Petunia hybrida: inducers of the vir region of the Agrobacterium tumefaciens Ti plasmid. Plant Sci 62: 83-91 (1989).